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Rajat Chaudhary Anurag Mishra Kapil Kumar Sonam Arya

Abstract

Chickpea (Cicer arietinum L.) is a legume which is mostly cultivated in India than other countries that can give significant amounts of dietary minerals and protein to humans. The effect of paper industrial effluent on chick-pea (C. arietinum L.) were analysed along with different concentration (10%, 20% 40%, 60% 80% and 100%) and pure tap water as a control to compare the effect of paper industrial effluent for one week. The amount of protein were comparable with control, their amount was increased at 40% in effluent treated seeds. The maximum activity of enzymes was found below 40% level of effluent. In this study protein profile of imbibed seeds, shoot, root and residual cotyledons were examined under the different concentration of effluent. Polyacrylamide gel electrophoresis of total protein showed that the maximum number of protein bands seen in the imbibed seeds whereas minimum number of protein bands observed in the root. SDS-PAGE revealed that less degradation and/or more rapid accumulation of proteins occurred in higher molecular weight proteins. From this study, it is clear that the industrial effluent rich in organic matter and plant nutrients are finding their use in agriculture as the cheaper way of disposal.

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Keywords

Amylase, Chickpea, Imbibed seeds, Paper industry effluent, SDS-PAGE

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Chaudhary, R., Mishra, A., Kumar, K., & Arya, S. (2017). Effect of paper industry effluent on enzyme activity and protein profiling of Chickpea (Cicer arietinum L.). Journal of Applied and Natural Science, 9(4), 1910–1915. https://doi.org/10.31018/jans.v9i4.1462
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