Hydropriming -a useful technique for seed invigoration in okra (Abelmoschus esculentus) and parsley (Petroselinum crispum)
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Abstract
Seed germinating ability and stand establishment determine the management options in crop production system. Physiological advancement of seeds through priming is a simple and cost-effective method to improve the germinability and stand of any crop. Studies were performed on seed invigoration through hydropriming at the division of PHT, SKUAST-Kashmir during the year 2016 to optimize the hydropriming duration for improved germi-nation and seedling vigour of okra (Pusa sawani) and parsley (curly type). Freshly harvested seeds were soaked in normal water for varying durations (okra- 12, 18 and 24 h; parsley- 24, 48 and 72 h) at (25±2°C) and re-dried to original moisture content at room temperature. Fifty seeds of each treatment were cultured in 14.0 cm Petri dishes lined with 5 layers of moist blotting paper. Observations on various germination parameters were recorded at (25±2°C). Priming of okra seeds for 18 h resulted in highest FGP (85.7%), GE (66.5%), GI (36.7), SDB (29.1mg) and SVI (2.49) coupled with minimum values of T50 (2.75 day) as well as MGT (2.38 day). However, the highest FGP (78.7%), GE (48.7%), SDB (3.13 mg), GI (12.8) and SVI (0.25) together with minimum T50 (7.2 day) and MGT (6.5 day) in parsley was recorded with 24 h priming duration. As such priming duration of 18 and 24 hours at 25±2°C were found optimal for enhanced and rapid seed germination with vigourous seedlings.
Article Details
Article Details
Keywords
Hydropriming, Okra, Parsley, Seed germination, Seedling vigour
References
Abbasdokht, H. (2011). The effect of hydropriming and halopriming on germination and early growth stage of wheat (Triticum aestivum L.). Desert 16: 61-68
Abbasdokht, H., Mazaheri, D., Chaichi, M., Rahimian, H., Baghestani, M. A., Mohammad Alizadeh, H. and Sharifzadeh, F. (2004). The evaluation of redroot pigweed (Amaranthus retroflexus L.) competition at different density and different time of germination on some of agronomical traits of soybean (Glycine max L.). Desert 8, 104-120
Abdul-Baki, A.A. and Anderson, J. D. (1973). Vigour determination in soybean by multiple criteria. Crop Science, 13: 630-633
Ando, H. and Kobata, T. (2002). Effect of seed hardening on the seedling emergence and ?-amylase activity in the grains of wheat and rice sown in dry soil. Japanese Journal of Crop Science, 71(2): 220-225
AOSA (Association of Official Seed Analysts). (1983). Seed vigour testing handbook. Contribution No. 32 to the Handbook of Seed Testing Lincoln, NE
AOSA (Association of Official Seed Analysts). (1990). Rules for testing seeds. Journal of Seed Technology, 12: 1–112
Ashraf, M. and Foolad, M.R. (2005). Pre-sowing seed treatment: A shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Advances in Agron. 88:223-271
Bam, R.K., Kumaga, F.K., Ori, K. and Asiedu, E.A. (2006). Germination, vigour and dehydrogenase activity of naturally aged rice (Oryza sativa L.) seeds soaked in potassium and phosphorus. Asian Journal of Plant Sciences, 5: 948-955
Bradford, K. J. (1986). Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience, 21:1105-1112
Bradford, K. J., Chen, F., Cooley, M.B. Dahal, P., Downie, B., Fukunaga, K.K., Gee, O.H. Gurusinghe, S., Mella, R.A, Nonogaki, H., Wu, C.T., Yang, H. and Yim, K.O. (2000). Gene expression prior to radicle emergence in imbibed tomato seeds, pp. 231-251. In: Black, M., Bradford K. J. and Va´zquez-Ramos J. (Eds.). Seed Biology: Advances and Applications. CABI International. Wallingford, UK
Cantliffe, D.J. (2003). Seed enhancement. ISHS Acta Horticulturae 607: IX International Symposium on Timing of Field Production in Vegetable Crops, Sao Paulo, 13(1):34
Caseiro, R., Bennett, M.A. and Marcos-Filho, J. (2004). Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed Sci. Technol. 32: 365–375
Coolbear P., Francis, A. and Grierson, D. (1984). The effect of low temperature pre-sowing treatment under the germination performance and membrane integrity of artificially aged tomato seeds. J. Experimen. Botan. 35: 1609-1617
De Castro, R.D., van Lammeren, A.A.M. Groot, S.P.C., Bino, R.J. and Hilhorst, H.W.M. (2000). Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not. Plant Physiol. 122: 327 - 335
Duman, I. and Esiyok, D. (1998) Ekim oncesi PEG ve KH2PO4 uygulamalar?n?n havuc tohumlar?n?n çimlenme ve c?k?s oran? ile verim üzerine etkileri. Turkish Journal of Agriculture and Forestry, 22: 445-449
Ellis, R.A. and Roberts, E.H. (1981). The quantification of aging and survival in orthodox seeds. Seed Sci. Technol. 9:373-409
Enu-Kwesi L., Nwalozie M. and D. I. and Anyanwu, D.I. (1986). Effect of Pre-sowing ‘hydration-dehydration’ on germination, vegetative growth and fruit yield of Abelmoschus esculentus under two soil moisture regimes. Tropical Agriculture (Trinidad), 63 (3): 181-184
Farooq, M., Basra, S.M.A., Hafeez, K. and Ahmad, N. (2005). Thermal hardening: a new seed vigour enhancement tool in rice. Acta Botanica Sinica, 47: 187-193
Ghassemi-Golezani, K., Chadordooz-Zeddi, A. and Nasrollahzadeh Moghaddam, M. (2010). Effects of hydro-priming duration on seedling vigour and grain yield of pinto Bean (Phaseolus vulgaris L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj., 38 (1): 109-113
Ghassemi-Golezani, K. and Mardfar, R. A. (2008). Effects of limited irrigation on growth and grain yield of common bean. J. Plant Sci. 3:230-235
Groot, S. P.C., Kieliszewska-Rokicha, B., Vermeer, E and Karssen, C.M. (1988). Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion. Planta. 174:500-504
Huang, R., Sukprakarn, S., Thongket, T. and Juntakool, S. (2002). Effect of hydropriming and redrying on the germination of triploid watermelon seeds. Kasetsart J. (Nat. Sci.), 36: 219 – 224.
Job, D., Capron, I., Job, C. Dacher, F., Corbineau, F. and Come, D (2000). Identification of germination-specific protein markers and their use in seed priming technology, pp. 449- 459. In: Black M., Bradford K. J. and Va´zquez-Ramos J. (Eds.). Seed Biology: Advances and Applications. CAB International. Wallingford, UK.
Khafagy, M.A., Darowish, M.M., Salama, S.M. and Abo-El-Kheer, E.A.M. (2014). Effect of water priming duration on rice (Oryza sativa L.) germination and seedling growth under ISO-osmotic solution of NaCl and PEG. J. Plant Production, Mansoura Univ., 5(12):2141 -2157
Matsushima K and Sakagami J. (2013). Effects of seed hydropriming on germination and seedling vigor during emergence of rice under different soil moisture conditions. American Journal of Plant Sciences, 4: 1584-1593
Mc-Donald, M.B. (1999). Seed deterioration: Physiology, repair and assessment. Journal of Seed Science Technology 27: 177-273.
Ogbuehi, H.C., Madukwe, D.K. and Ashilonu, P. (2013). Assessment of hydropriming of seeds on performance of morphological indices of bambara groundnut (Vigna subterrenealinn) landrace. G.J B.A.H.S., 2(2):17-22
Pandita, V.K., Anand, A., Nagarajan, S., Seth, R., Sinha, S.N. (2010). Solid matrix priming improves seed emergence and crop performance in okra, Seed Science and Technology, 38: 665-674
Vural, H., E?iyok, D. and Duman, I. (2000). Cultural vegetables, [Kültür sebzeleri (sebze yeti?tirme)] Ege University Publications. 166. ISBN: 975-97190-0-2
Abbasdokht, H., Mazaheri, D., Chaichi, M., Rahimian, H., Baghestani, M. A., Mohammad Alizadeh, H. and Sharifzadeh, F. (2004). The evaluation of redroot pigweed (Amaranthus retroflexus L.) competition at different density and different time of germination on some of agronomical traits of soybean (Glycine max L.). Desert 8, 104-120
Abdul-Baki, A.A. and Anderson, J. D. (1973). Vigour determination in soybean by multiple criteria. Crop Science, 13: 630-633
Ando, H. and Kobata, T. (2002). Effect of seed hardening on the seedling emergence and ?-amylase activity in the grains of wheat and rice sown in dry soil. Japanese Journal of Crop Science, 71(2): 220-225
AOSA (Association of Official Seed Analysts). (1983). Seed vigour testing handbook. Contribution No. 32 to the Handbook of Seed Testing Lincoln, NE
AOSA (Association of Official Seed Analysts). (1990). Rules for testing seeds. Journal of Seed Technology, 12: 1–112
Ashraf, M. and Foolad, M.R. (2005). Pre-sowing seed treatment: A shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Advances in Agron. 88:223-271
Bam, R.K., Kumaga, F.K., Ori, K. and Asiedu, E.A. (2006). Germination, vigour and dehydrogenase activity of naturally aged rice (Oryza sativa L.) seeds soaked in potassium and phosphorus. Asian Journal of Plant Sciences, 5: 948-955
Bradford, K. J. (1986). Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience, 21:1105-1112
Bradford, K. J., Chen, F., Cooley, M.B. Dahal, P., Downie, B., Fukunaga, K.K., Gee, O.H. Gurusinghe, S., Mella, R.A, Nonogaki, H., Wu, C.T., Yang, H. and Yim, K.O. (2000). Gene expression prior to radicle emergence in imbibed tomato seeds, pp. 231-251. In: Black, M., Bradford K. J. and Va´zquez-Ramos J. (Eds.). Seed Biology: Advances and Applications. CABI International. Wallingford, UK
Cantliffe, D.J. (2003). Seed enhancement. ISHS Acta Horticulturae 607: IX International Symposium on Timing of Field Production in Vegetable Crops, Sao Paulo, 13(1):34
Caseiro, R., Bennett, M.A. and Marcos-Filho, J. (2004). Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed Sci. Technol. 32: 365–375
Coolbear P., Francis, A. and Grierson, D. (1984). The effect of low temperature pre-sowing treatment under the germination performance and membrane integrity of artificially aged tomato seeds. J. Experimen. Botan. 35: 1609-1617
De Castro, R.D., van Lammeren, A.A.M. Groot, S.P.C., Bino, R.J. and Hilhorst, H.W.M. (2000). Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not. Plant Physiol. 122: 327 - 335
Duman, I. and Esiyok, D. (1998) Ekim oncesi PEG ve KH2PO4 uygulamalar?n?n havuc tohumlar?n?n çimlenme ve c?k?s oran? ile verim üzerine etkileri. Turkish Journal of Agriculture and Forestry, 22: 445-449
Ellis, R.A. and Roberts, E.H. (1981). The quantification of aging and survival in orthodox seeds. Seed Sci. Technol. 9:373-409
Enu-Kwesi L., Nwalozie M. and D. I. and Anyanwu, D.I. (1986). Effect of Pre-sowing ‘hydration-dehydration’ on germination, vegetative growth and fruit yield of Abelmoschus esculentus under two soil moisture regimes. Tropical Agriculture (Trinidad), 63 (3): 181-184
Farooq, M., Basra, S.M.A., Hafeez, K. and Ahmad, N. (2005). Thermal hardening: a new seed vigour enhancement tool in rice. Acta Botanica Sinica, 47: 187-193
Ghassemi-Golezani, K., Chadordooz-Zeddi, A. and Nasrollahzadeh Moghaddam, M. (2010). Effects of hydro-priming duration on seedling vigour and grain yield of pinto Bean (Phaseolus vulgaris L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj., 38 (1): 109-113
Ghassemi-Golezani, K. and Mardfar, R. A. (2008). Effects of limited irrigation on growth and grain yield of common bean. J. Plant Sci. 3:230-235
Groot, S. P.C., Kieliszewska-Rokicha, B., Vermeer, E and Karssen, C.M. (1988). Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion. Planta. 174:500-504
Huang, R., Sukprakarn, S., Thongket, T. and Juntakool, S. (2002). Effect of hydropriming and redrying on the germination of triploid watermelon seeds. Kasetsart J. (Nat. Sci.), 36: 219 – 224.
Job, D., Capron, I., Job, C. Dacher, F., Corbineau, F. and Come, D (2000). Identification of germination-specific protein markers and their use in seed priming technology, pp. 449- 459. In: Black M., Bradford K. J. and Va´zquez-Ramos J. (Eds.). Seed Biology: Advances and Applications. CAB International. Wallingford, UK.
Khafagy, M.A., Darowish, M.M., Salama, S.M. and Abo-El-Kheer, E.A.M. (2014). Effect of water priming duration on rice (Oryza sativa L.) germination and seedling growth under ISO-osmotic solution of NaCl and PEG. J. Plant Production, Mansoura Univ., 5(12):2141 -2157
Matsushima K and Sakagami J. (2013). Effects of seed hydropriming on germination and seedling vigor during emergence of rice under different soil moisture conditions. American Journal of Plant Sciences, 4: 1584-1593
Mc-Donald, M.B. (1999). Seed deterioration: Physiology, repair and assessment. Journal of Seed Science Technology 27: 177-273.
Ogbuehi, H.C., Madukwe, D.K. and Ashilonu, P. (2013). Assessment of hydropriming of seeds on performance of morphological indices of bambara groundnut (Vigna subterrenealinn) landrace. G.J B.A.H.S., 2(2):17-22
Pandita, V.K., Anand, A., Nagarajan, S., Seth, R., Sinha, S.N. (2010). Solid matrix priming improves seed emergence and crop performance in okra, Seed Science and Technology, 38: 665-674
Vural, H., E?iyok, D. and Duman, I. (2000). Cultural vegetables, [Kültür sebzeleri (sebze yeti?tirme)] Ege University Publications. 166. ISBN: 975-97190-0-2
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Hydropriming -a useful technique for seed invigoration in okra (Abelmoschus esculentus) and parsley (Petroselinum crispum). (2017). Journal of Applied and Natural Science, 9(3), 1792-1795. https://doi.org/10.31018/jans.v9i3.1440
