Assessment of genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers

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Published Sep 1, 2017
DOI: https://doi.org/10.31018/jans.v9i3.1433

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Amit Kumar
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
R. S. Sengar
Department of Agriculture Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technolo-gyMeerut-250110(U.P) , INDIA
Vivekanand Pratap Rao
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
Gyanika Shukla
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
Rekha Dixit
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
Raj Singh
Department of Botany, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP) , INDIA

Abstract

The present study aimed to evaluate the genetic diversity of 10 wheat cultivars by Random Amplified Pol-ymorphic DNA (RAPD) marker. The genomic DNA of 10 wheat genotypes were amplified with 10 RAPD primers that produced 53 amplified band, out of which 23 band were polymorphic (43.39%). The number of fragment amplified per primer ranged from 4 to 9. Primer A01 generated maximum number of amplified band, out of which 5 band were polymorphic. Cluster analysis of wheat genotypes were based on UPGMA method. Cluster analysis of 10 wheat genotypes were classified in to two main group; single variety AKW 1071 was placed in first group and rest 9 variety were placed in second group. The pair wise similarity values ranged from 0.58% to 100% and showed that cultivars Raj-3765 and K-7903 were the closest with highest similarity value (100%), while genotypes AKW 1071 and K9006 showed minimum similarity value (62%). The present study indicated the presence of high genetic diversity among wheat cultivars, which could be used for the developing core collection of wheat germplasm for breeding purpose.

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Article Details

Keywords

Cluster analysis, RAPD marker, UPGMA method, Wheat genotypes

References
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Issue
Vol 9 No 3 (2017)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Assessment of genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers. (2017). Journal of Applied and Natural Science, 9(3), 1751-1755. https://doi.org/10.31018/jans.v9i3.1433