Assessment of genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers

##plugins.themes.bootstrap3.article.sidebar##

PDF
Published Sep 1, 2017
DOI: https://doi.org/10.31018/jans.v9i3.1433

##plugins.themes.bootstrap3.article.main##

Amit Kumar
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
R. S. Sengar
Department of Agriculture Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technolo-gyMeerut-250110(U.P) , INDIA
Vivekanand Pratap Rao
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
Gyanika Shukla
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
Rekha Dixit
Department of Biotechnology, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP), INDIA
Raj Singh
Department of Botany, Faculty of Science, Swami Vivekanand Subharti University, Meerut-250005 (UP) , INDIA

Abstract

The present study aimed to evaluate the genetic diversity of 10 wheat cultivars by Random Amplified Pol-ymorphic DNA (RAPD) marker. The genomic DNA of 10 wheat genotypes were amplified with 10 RAPD primers that produced 53 amplified band, out of which 23 band were polymorphic (43.39%). The number of fragment amplified per primer ranged from 4 to 9. Primer A01 generated maximum number of amplified band, out of which 5 band were polymorphic. Cluster analysis of wheat genotypes were based on UPGMA method. Cluster analysis of 10 wheat genotypes were classified in to two main group; single variety AKW 1071 was placed in first group and rest 9 variety were placed in second group. The pair wise similarity values ranged from 0.58% to 100% and showed that cultivars Raj-3765 and K-7903 were the closest with highest similarity value (100%), while genotypes AKW 1071 and K9006 showed minimum similarity value (62%). The present study indicated the presence of high genetic diversity among wheat cultivars, which could be used for the developing core collection of wheat germplasm for breeding purpose.

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Cluster analysis, RAPD marker, UPGMA method, Wheat genotypes

References
Ali, Ammar., Ali, Nawab, Ali, Imran., Adnan, Muhammad., Ullah, Nimat., Ahmed Zahoor (2013) Morphological and genetic diversity of Pakistani Wheat germplasm under drought stress. International Journal of Advancements in Research & Technology.2(5):186-193.
Demeke, T., Laroche, A., Gaudet, D.A. (1996). A DNA marker for the Bt-10 common bunt resistance gene in wheat. Genome J. 39: 51–55.
Doyle, J. J. and Doyle, J. L. (1987) A rapid DNA isolationbprocedure for small quantities of fresh leaf tissue. Phyto. Bull.b19: 11–15.
Farrakh, S., Ahmad, Mirza, J. I., Hameed, S., Kazi, M., Ashraf, M. (2011) RAPD analysis of stripe rust resistant synthetic hexaploid of wheat. Int. J. Appl. Sci. Technol. 1:3.
Grewal, S., Kharb, P., Malik, R., Jain, S., Jain, R.K. (2007) Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers. Indian J. Biotech. 6:18-33.
Hemrick, J.L., and Godt, M.J.W. (1990) Alloenzyme diversity in plant species, In: Plant Population Genetics, Breeding and Genetic Resources. (Eds.): A.H.D. Brown, M.T. Clegg, A.L. Kahler, B.S. Weir. Sinauer Associates Inc., Sunderland MA, pp. 43-63.
Jaccard, P. (1908) Nouvelles reserches sur la distribution florale. Bulletin Societe Vaudoise Des Sciences Naturelles. 44: 223-270.
Kumar, A., Singh, R., Singh, R., and Sengar, S. R. (2015). Molecular Approach for Detection of Plant pathogen. An International Journal of Biological Sciences Biotech today. 5(2):14 -19.
Kumar, S., Deepali, Mishra D. C., Bansal R., Kumari J., Kumar S. (2016) Molecular characterization of Indian wheat germplasm line for stay green & other heat tolerance genes using linked SSR markes. An International Jouranl of Biological Sciences Biotech today. 6(1):90 -94.
Mahpara, S., Farooq, J., Ali, Z., Petrescu-Mag IV., Hussain, F. (2012) Assessment of genetic distance among wheat genotypes through RAPDmarkers. Advances in Agriculture and Botanics J. 4(1):31–35.
Nei, M., Li, W. H. (1979) Mathematical models for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76:5269-5273.
Parveen, S., Saharan, M.S., Verma., A and Sharma, I. (2013). Comparative analysis of RAPD and ISSR marker assays for detecting genetic polymorphism in Tilletia indica. European Journal of Experimental Biology 3(1):380-387.
Siddiqui, M. F., Iqbal, S., Erum, S., Naz, Khan, S. (2010) DNA landmarks for genetic relatedness and diversity assessment in Pakistani wheat genotypes using RAPD markers. Pak. J. Bot. 42(2):1013-1020.
Sivolap, Y.M., Chebotar, S.V., Topchieva, E.A., Korzun,V. N., and Totskiy, V. N. (1999) RAPD and SSRP analyses of molecular-genetic polymorphism in Triticum aestivum L. cultivars. Russian J. Genet. 35: 1433—1440.
Taran, B., Zhang C., Warkentin T., Tullu, A., and Vandenberg (2005) A genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.) based on molecular markers, and morphological and physiological characters. Genome, 48: 257-272.
Issue
Vol 9 No 3 (2017)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Assessment of genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers. (2017). Journal of Applied and Natural Science, 9(3), 1751-1755. https://doi.org/10.31018/jans.v9i3.1433