Anita Puyam Shikha Sharma Prem Lal Kashyap


RNA interference (RNAi) is an incredible revolution in the field of functional genomics, a breakthrough in plant molecular genetics. This technology will generate enormous potential for engineering control of gene expres-sion. The success of managing biotic stress using RNAi technology will prove to be biologically and environmentally safe. It is therapeutic in approach as the resistance induced by RNAi is triggered by ds RNA that results in silencing of specific genes before being translated in a homology dependent manner. Over the time, RNAi is significantly proving it as one of the most promiscent management strategy which eliminates certain risks associated with the development of transgenic plants. This review gives an insight into the probability of management of plant diseases caused by various biotic agents viz. fungi, bacteria and viruses using RNA interference technique and host-pathogen related targeted sites.




Diseases, Functional genomics, Gene Expression, Management, RNAi

Aragao, F.J.and Faria, J.C. (2009). First transgenic geminivirus-resistant plant in the field. Nat. Biotechnol., 27:1086–1088.
Baulcombe D. (2004). RNA silencing in plants. Nature.431: 356-63.
Bertazzon, N., Raiola, A., Castiglioni, C. ,Gardiman, M., Angelini, E., Borgo, M. and Ferrari, S. (2012). Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.Plant Cell Rep., 31:133-43.
Brodersen P., and Voinnet O. (2006). The diversity of RNA silencing pathways in plants. Trends Genet. 22: 268-80.
Broglie, K.I., Chet, M., Holliday, M.N. (1991). Transgenic plants with enhanced resistance to fungal pathogen Rhizoctonia solani. Science, 254: 1194.
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (1996). Plant viruses online: descriptions and lists from the VIDE database. 2011-04-20 .http://biology. anu. edu. au/Groups/MEs/vide.
Cakir C. and Tör, M. (2010) Factors Influencing Barley Stripe Mosaic Virus-Mediated Gene Silencing in Wheat. Physiol. Mol. Plant Pathol. 74: 246-253.
Chuang, C.F. and Meyerowtiz, E.M. (2000). Specific and Heritable Genetic Interference by Double-Stranded RNA in Arabidopsis thaliana. PNAS, 97:4985- 4990.
Constantin, G. D., Krath, B. N., MacFarlane, S. A., Nicolaisen, M., Johansen, I. E. and Lund, O. S. (2004) Virus-Induced Gene Silencing as a Tool for Functional Genomics in a Legume Species. Plant J., 40: 622-631.
Dalmay, T., Hamilton, A.J., Mueller, E. and Baulcombe, D.C. (2000). Potato Virus X Amplicons in Arabidopsis Mediate Genetic and Epigenetic Gene Silencing. PlantCell 12:369-380.
Dimitar, D., Stefanie, L., Annika, J., Daniela, Axel, H., Jeyaraman, R., Nils, S., Rajiv, S., Benjamin, K. and Patrick, S. (2014). Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi. Genome boil., 15:518.
Ding, X. S., Schneider, W. L., Chaluvadi, S. R., Rouf Mian, R. M. and Nelson, R. S. (2006) Characterization of a Brome Mosaic Virus Strain and Its Use as a Vector for Gene Silencing in Monocotyledonous Hosts. Mol. Plant-Microbe Interact., 19: 1229-1239.
Duan, C.G.,Wang, C.H. and Guo, H.S. (2012). Application of RNA silencing to plant disease resistance. Silence, 3: 1-8.
Dunoyer, P., Himber, C., Ruiz-Ferrer, V., Alioua, A. and Voinnet, O. (2007). Intra- and Intercellular RNA Interference in Arabidopsis thaliana Requires Components of the MicroRNA and Heterochromatic Silencing Pathways. Nat.Genet., 39:848-856.
Escobar, M.A., Civerolo, E.L., Summerfelt, K.R. and Dandekar, A.M. (2001). RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis. PNAS, 98:13437-13442.
Fagwalawd, I.D., Kutama, A.S., Yakasai, M.T. (2013). Current issues in plant disease control: biotechnology and plant disease. Bajopas, 6: 121-126.
Faivre-Rampant, O., Gilroy, E. M., Hrubikova, K., Hein, I. Millam, S., Loake, G. J., Birch, P., Taylor, M. and Lacomme, C. (2004) Potato Virus X-Induced Gene Silencing in Leaves and Tubers of Potato. Plant Physiol., 134: 1308-1316.
Fillati, J.J., Kiser, J., Rose, R. and Comai, L. (1987). Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Biotechnol., 5: 726-730.
Fitzgerald, A., Van, Kha, J.A. and Plummer, K.M. (2004). Simultaneous Silencing of Multiple Genes in the Apple Scab Fungus Venturia inaequalis, by Expression of RNA with Chimeric Inverted Repeats. Fungal Genet.Biol., 41: 963-971.
Fofana, I. B., Sangare, A., Collier, R., Taylor, C. and Fauquet, C. M. (2004) A Geminivirus-Induced Gene Silencing System for Gene Function Validation in Cassava. Pl. Mol. Biol., 56: 613-624
Gan, D., Zhang, J., Jiang, H., Jiang, T., Zhu, S. and Cheng, B. (2010). Bacterially expressed dsRNA protects maize against SCMV infection.Plant Cell Rep., 29:
Gossele, V. V., Fache, I. I., Meulewaeter, F., Cornelissen, M. and Metzlaff, M. (2002) SVISS-a Novel Transient Gene Silencing System for Gene Function Discovery and Validation in Tobacco. The. Plant. J., 32: 859- 866.
Gould, B. and Kramer, E. M. (2007) Virus-Induced Gene Silencing as a Tool for Functional Analyses in the Emerging Model Plant Aquilegia (columbine, Ranunculaceae). BMC Plant Methods, 12: 6.
Hily, J.M. and Liu, Z. (2007). An Overview of Small RNAs. In: Bassett, C, L.( eds). Regulation of Gene Expression in Plants. Springer-Verlag, Berlin. pp. 123-147.
Hou, H. and Qiu, W. (2003) A Novel Co-Delivery System Consisting of a Tomato Bushy Stunt Virus and a Defective Interfering RNA for Studying Gene Silencing,” J. Virol. Methods, 111: 37-42.
International service for acquisition of agri-biotech applications (2012). Pocket K No. 34: RNAi for Crop Improvement. Available Source: http://isaaa.org/resources/publications/pocketk/34/default.asp
Jiang, C.J., Shimono, M., Maeda, S., Inoue, H., Mori, M., Hasegawa, M., Sugano, S. and Takatsuji, H. (2009). Suppression of the rice fatty-acid desaturase gene OsSSI2 enhances resistance to blast and leaf blight diseases in rice. Mol. Plant-Microbe Interact., 22: 820-829.
Kadotani, N., Nakayashiki, H.,Tosa, Y. and Mayama, S. (2003). RNA Silencing in the Pathogenic Fungus Magnaportheoryzae. Mol. Plant-Microbe Interact., 16:769-776.
Khraiwesh, B., Zhu, J.K. and Zhu, J. (2012). siRNAs in biotic and abiotic stress responses of plants.Biochim. Biophys. Acta, 1819: 137–148.
Kjemtrup. S., Sampson, K.S., Peele, C.G., Nguyen, L.V. and Conkling, M.A. (1998). Gene Silencing from Plant DNA Carried by a Geminivirus. Plant J., 14:91-100.
Kumagai, M. H., Donson, J., della-Cioppa, G., Harvey, D., Hanley, K. and Grill, L. K. (1995) Cytoplasmic Inhibition of Carotenoid Biosynthesis with Virus-Derived RNA. Proceedings of the National Academy of Sciences of the USA. 92: 1679-1683.
Li, X., Wang, X., Zhang, S., Liu, D., Duan, Y. and Dong, W. (2012). Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing. PLoS ONE doi:10.1371/journal.pone. 0039650.
Liu, Y.L., Schiff, M. and Dinesh-Kumar S.P. (2002). Virus Induced Gene Silencing in Tomato. Plant J.31:777-786.
Maloy, O.C. (2005). Plant disease management. Plant Instructor. D01:10 1094/PHL – 1 – 2005– 0202 – 01.
Mann, S.K., Kashyap, P.L., Sanghera, G.S., Singh, G. and Singh, S. (2008). RNA Interference: An eco-friendly tool for plant disease management. Transgen. Plant J., 2:110-126
Mohanpuria, P., Rana, N. and Yadav, S. (2008). Transient RNAi based gene silencing of glutathione synthetase reduces glutathione content in Camellia sinensis(L.) O. Kuntze somatic embryos. Biol. Plant., 52: 381-384.
Moritoh, S., Miki, D., Akiyama, M., Kawahara, M., Izawa, T., Maki, H. and Shimamoto, K. (2005). RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant Cell Physiol., 46:699-715.
Naylor, M., Reeves, J., Cooper, J. I., Edwards, M. L. and Wang, H. (2005) Construction and Properties of a Gene Silencing Vector Based on Poplar Mosaic Virus (Genus Carlavirus). J. Virol. Methods, 124: 27-36
Nowara D., Gay, A., Lacomme, C., Shaw, J., Ridout, C., Douchkov, D., Hensel, G., Kumlehn, J., Schweizer, P. (2010). HIGS: Host Induced gene silencing in the obligate biotrophic fungal pathogen Blumeria graminis. Plant Cell., 22: 3130-3141.
Palmer, K.E. and Rybicki, E.P. (2001). Investigation of the potential of maize streak virus to act as an infectious gene vector in maize plants. Arch. Virol., 146:1089-1104.
Pandolfini, T., Molesini, B., Avesani, L., Spena, A. and Polverari, A. (2003). Expression of self-complementary hairpin RNA under the control of the rolC promoter confers systemic disease resistance to Plum pox virus without preventing local infection. BMC
Biotechnol., 3:7.
Peele, C., Jordan, C.V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L. and Robertson, D. (2001) Silencing of a Meristematic Gene Using Geminivirus-Derived Vectors. Plant J., 27: 357-366.
Persengiev, S. P., Zhu, X. and Green, M.R. (2004).Non-specific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs). RNA,10: 12–18.
Pooggin, M.., Shivaprasad, P.V., Veluthambi, K. and Hohn, T. (2003). RNAi targeting of DNA virus in plants. Nat. Biotechnol., 21:131–132.
Ratcliff, F., Martin-Hernandez, A. M. and Baulcombe, D. C. (2001) Tobacco Rattle Virus as a Vector for Analysis of gene Functions by Silencing. Plant J., 25: 237-245.
Romano, N. and Macino, G. (1992). Quelling: transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences. Mol. Microbiol., 6:3343-3353.
Ruiz, M. T., Voinnet, O. and Baulcombe, D. C. (1998) Initiation and Maintenance of Virus-Induced Gene Silencing. Plant Cell, 10: 937-946.
Sanghera, G. S., Kashyap, P. L., Singh, G., Teixeira da Silva, J.A. (2011).Transgenics: fast track to plant stress amelioration. Transgen. Plant., J.5 :1-26.
Schwab, R. (2006). Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis. Plant Cell, 18:1121–1133.
Schweizer, P., Pokorny, P., Schulze-Lefert, P. and Dudler ,R .(2000). Double Stranded RNA Interference with Gene Functions at the Single Cell in Cereals. Plant J., 24: 895-903.
Scofield, S.R., Huang, L., Brandt, A.S. and Gill, B.S. (2005). Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiol., 138: 2165-2173.
Seemanpillai, M., Dry, I., Randles, J. and Rezaian, A. (2003). Transcriptional silencing of geminiviral promoter-driven transgenes following homologous virus infection. Mol. Plant-Microbe Interact.,16:429–438.
Segers, G.C., Hamada, W., Oliver, R.P. and Pspanu, P.D. (1999). Isolation and Characteristaion of Five Different Hydrophobin-Encoding cDNA from the Fungal Tomato Pathogen Cladosporium fulvum. Mol.Gen. Genet., 261:644-652.
Senthil-Kumar, M. and Mysore, K.S. (2010). RNAi in Plants: recent developments and applications in agriculture. Gene Silencing: Theory, Techniques and Applications, 83-199.
Singh, R.S. (2005). Introduction to Principles of Plant Pathology. Oxford and IBH Publishing Co. PVT. LTD., New Delhi. pp.178-189.
Smith, N.A., Singh, S.P., Wang, M.B., Stoutjesdijk, P.A., Green, A.G. and Waterhouse, P.M. (2000). Total silencing by intron-spliced hairpin RNAs. Nature, 407:319–320.
Tao, X. and Zhou, X. (2004) A Modified Viral Satellite DNA that Suppresses Gene Expression in Plants. The Plant J. 38: 850-860.
Tenllado, F. and Diaz-Ruiz, J.R. (2001). Double-stranded RNA-mediated interference with plant virus infection. J. Virol.75: 12288–12297.
Turnage, M. A., Muangsan, N., Peele, C. G. and Robertson, D. (2002) “Geminivirus-Based Vectors for Gene Silencing in Arabidopsis. The Plant J. 30: 107-117.
Wani, S. H., Sanghera, G.S. and Singh, N.B. (2010). Biotechnology and Plant Disease Control-Role of RNA Interference. Am. J. Plant Sci.1: 55-68.
Waterhouse, P.M., Graham, M.W. and Wang, M.B. (1998). Virus Resistance and Gene Silencing in Plants can be Induced by Simultaneous Expression of Sense and Antisense RNA. PNAS95: 13959-64.
Wesley,V., Helliwell, C., Smith, N., Wang, M., Rouse, D., Liu, Q., Gooding, P., Singh, S., Abbott, D., Stoutjesdijk, P., Robinson, S., Gleave, A., Green, A. and Waterhouse P (2001). Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27:581-590.
Wroblewski, T., Piskurewicz, U., Tomczak, A. and Ochoa Michelmore , R.W. (2007). Silencing of the major family of NBS-LRR-encoding genes in lettuce results in the loss of multiple resistance specificities. Plant J. 5:803-818.
Yin, C., Jurgenson, J.E. and Hulbert, S.H. (2011). Development of a host-induced RNAi system in the wheat Stripe Rust Fungus Puccinia striiformis f.sp.tritici.Mol. Plant-Microbe Interact. 24: 554-561.
Yin, G.H., Sun, Z.N, Song YZ, An HL, Zhu CX, Wen FJ(2010). Bacterially expressed double-stranded RNAs against hot-spot sequences of tobacco mosaic virus or potato virus Y genome have different ability to protect tobacco from viral infection.Appl. Biochem. Biotechnol. 162:1901-1914.
Yuan, B., Latek, R., Hossbach, M., Tuschl, T. and Lewitter, F. (2004). siRNA Selection Server: an automated siRNA oligonucleotide prediction server. Nucleic Acids Res.32: 130–134.
Zhai, J., Jeong , D.H., DePaoli, E., Park, S., Rosen, B.D. and Li, Y. (2011). Micro RNAs as master regulators of the plant NB-LRR defense gene family via the production of phased, trans- acting siRNAs. Genes Dev. 25:2540–2553.doi: 10.1101/gad.177527.111
Zhang C. and Ghabrial, S. A. (2006) Development of Bean Pod Mottle Virus-Based Vectors for Stable Protein Expression and Sequence-Specific Virus-Induced Gene Silencing in Soybean. Virology 344: 401-411.
Zongli, H., Urvi, P., Natsumi, M., Yuri, T. and Jose, R.B. (2015) Downregulation of Fusariumoxysporum endogenousgenes by Host-Delivered RNAinterference enhances disease resistance. Frontiers in chem.3: 1-10
Zrachya, A, Kumar, P.P., Ramakrishan, U., Levy, Y., Loyter, A., Arazi, T., Lapidot, M. and Gafni, Yedidya (2007). Production of siRNA targettance to the virused against TYlC coat protein transcripts lead to silencing expression and resisistance in virus. Transgenic Res. 16: 385-98
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RNA interference- a novel approach for plant disease management. (2017). Journal of Applied and Natural Science, 9(3), 1612-1618. https://doi.org/10.31018/jans.v9i3.1410
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RNA interference- a novel approach for plant disease management. (2017). Journal of Applied and Natural Science, 9(3), 1612-1618. https://doi.org/10.31018/jans.v9i3.1410

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