Sheeja K Raj Elizabeth K Syriac K. N. Anith K. S. Meenakumari


he experiments were conducted in vitro in the Agricultural Microbiology laboratory at College of Agricul-ture, Vellayani, Thiruvananthapuram, Kerala, India to evaluate the compatibility of biocontrol agents Pseudomonas fluorescens and Tricoderma viride and N fixing organisms Azospirillum lipoferum and Azotobacter chroococcum to bispyribac sodium + metamifop 14 % SE, a new broad spectrum post emergence pre-mix herbicide mixture used for weed control in rice. The experiments were conducted in completely randomized block design with seven different concentrations of herbicide viz., 100, 120, 140, 160, 180, 200 and 220 ?L L-1 corresponding to field doses of 50, 60, 70, 80 90, 100 and 110 g ha-1 and a control (0 ?L L-1). All the tested concentrations of the herbicide mixture were highly compatible with P. fluorescens, A. lipoferum and A. chroococcum. The radial colony diameter of T. viride was significantly influenced by different concentrations of the herbicide. The field dose of bisspyribac sodium + met-amifop up to 90 g ha-1 (180 ?L L-1) is harmless to T. viride, since it recorded a growth inhibition of only 22.96 per cent but higher doses (100 and 110 g ha-1) which recorded a growth inhibition of 31.48 and 37.04 per cent respectively were slightly harmful to the antagonistic fungus. The compatibility results revealed the possibility of using bispyribac sodium + metamifop for weed control at recommended doses (70, 80 or 90 g ha-1) under bio intensive disease management programme involving P. fluorescens / T. viride and nutrient management programme involving A. liopferum / A chroococcum.




Bio control agents, Bispyribac sodium metamifop, Compatibility, Herbicide mixture, N fixing organisms

Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method.Am. J. Clin. Pathol. 36:493-496
Biswas, S. and Datta, M. (2013). Evaluation of biological control agents against sheath blight of rice in Tripura. Indian J. Phytopathol.66: 77-80
Brock, L. (1975). Effects of the Herbicides Trifluralin and Carbethamide on nodulation and growth of legume seedlings.Weed Res. 12: 150-145
Clarkson, J.P., Scruby, A., Mead, A., Wright, C., Smith, B and Whipps, J.M. (2006). Integrated control of Allium white rot with Trichoderma viride, tebuconazole and composted onion waste. Plant Pathol. 55: 375-386
Gadkari, D. and Klingmuller, W. (1988). Influence of herbicides on growth and nitrogenase activity of Azospirillum. In: AzospirillumIV. Genetics, Physiology, Ecology.Proceedings of the 4thAzospirillumWorkshop. Springer Verlag. pp. 150-158
Gangwar, G.P. (2013). Compatibility of bacterial bioagent for bacterial leaf blight of rice with chemical pesticides, commonly used in rice cultivation. Int. J. Appl. Nat. Sci. 5: 378-381
Gangwar, G.P. and Sinha, A.P. (2012). Comparative antagonistic potential of fungal and bacterial bioagentsagainst Xanthomonasoryzaepv. oryzae. Ann. Pl. Protec. Sci.20: 154-159
Hermosa, R., Viterbo, A., Chet, I. and Monte, E. (2012). Plant beneficial effects of Trichoderma and of its gene. Microbiol. 158: 17-25
Hirose, E., Neves, P.M.O.J., Zequi, J.A.C., Martins, L.H., Peralta, C.H., Junior, M. A. (2001). Effect of biofertilizers and neem oil on the entomopathogenic fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. Braz. Arch. Biol Technol. 44: 419-423
Jeenie, Sharma, P. and Khanna, V. (2011). In vitro sensitivity of Rhizobium and phosphate solubilizing bacteria to herbicides. Indian J. Microbiol. 51: 230-233
Kizilkaya, R. (2009) Nitrogen fixation capacity of Azotobacter spp. Strains isolated from soils indifferent ecosystems and relationship between them and the microbiological properties of soils. J. Environ. Biol. 30: 73-82
Krishnakumari, P. (2016). Antagonistic effect of Pseudomonas fluorescens on Rhizoctonia solani Kuhn. causing sheath blight disease in rice. Asian J. Sci. Technol. 7: 2364-2368
Martinez-Toledo, M.V., Salmeron, V. and Gonzalez-Lopez, J. (1990). Effect of phenoxy and benzoic acid herbicides on nitrogenase activity and growth of Azospirillum brasilense. Soil Biol. Biochem.22: 879-881
Mrkovacki, N.B., Cacic, N.A. and Malic, V.M. (2002).
Effects of pesticides on Azotobacter chroococcum. Proc. Natural Sci. Matica Srpska 102: 23-28
Neves, P.M.O.J., Hirose, E., Tchujo, P.T., MoinoJunio, A. (2001). Compatibility of entomopathogenic fungi with neonicotinoids insecticides. Neotrop. Entomol. 30:263-268
Poureidi, S., Yazdanpanah, M., Rokhzadi, A., Amri, M. and Fayazi, H. (2015). Effect of plant growth promoting bacteria (Azospirillum, Azotobacter, Pseudomonas), humic acid and nitrogen fertilizer on growth and yield of wheat. Bull. Environ. Pharmacol. Life Sci.4: 82-87
Rao, C.V.R. and Divakar, B.J. (2002). Effect of three pesticides on the fungal bioagent, Trichoderma viride.Frontiers Microbial Biotechnol. Pl. Path., 24: 183-186
Santoro, P.H., Cavaguchi, S.A., Alexandra, T.M., Zorzett, J. and Neves, M.O.J. (2014). In vitro sensitivity of antagonistic Trichoderma atroviride to herbicides .Braz. Arch. Biol. Technol. 57: 238- 243
Saravanakumar, D., Lavanya, N., Muthumeena, K., Raguchander, T. and Samiyappan, R. (2009). Fluorescent pseudomonad mixtures mediate disease resistance in rice plants against sheath rot (Sarocladium oryzae) disease. Biocontrol 54: 273–286
Sattar, M.A., Rahman, M.F., Das, D.K. and Choudhury, A.T.M.A. (2008). Prospects of using Azotobacter, Azospirillum and Cyanobacteria as supplements of urea nitrogen for rice production in Bangladesh. In. Proceedings of ACIR No.130, pp.59-66
Saxena, D., Tewari, A.K. and Rai, D. (2014). The in vitro effects of some commonly used fungicides, insecticides and herbicides for their compatibility with Trichoderma harzianum PBT 23. World Appl. Sci. J. 31: 444-448
Shyamala, L. and Sivakumar, P.K. (2012). Pseudomonas fluorescens mediate disease resistance in rice against rice blast caused by Pyriculariaoryzae. Int. J. Recent Sci. Res. 3: 853-857
Silva, R.Z. and Neves, P.M.O.J. (2005). Techniques and parameters used in compatibility tests between Beauveriabassiana(Bals) Vuill. And in vitro phytosanitary products. Pest Manag. Sci. 61: 667-674
Sterk, G., Heuts, F., Merck, N. and Bock, J. (2002). Sensitivity of non-target arthropods and beneficial fungal species to chemical and biological plant protection products: Results of laboratory and semi-field trials V. In: Driesche, R.G. van (ed.), Proceedings of the Ist International Symposium on biological control of Arthropods, 14-18 January, Honolulu, Hawaii, United States Department of Agriculture, Forest Service, Washington, USA, pp.306-313
Sundar, A.R., Das, N.D. and Krishnaveni, D. (1995). In-vitro antagonism of Trichoderma spp. against two fungal pathogens of Castor. Indian J. Plant Protec. 23:152-155
Surendran, M., Kannan, G.S., Nayar, K. and Leenakumary, S. (2012). Compatibility of Pseudomonas fluorescens with agrichemicals. J. Bio. Control. 26: 190-193
Zentmeyer, G.A. (1955). A laboratory method for soil fungicides with Phytophthora cinnamon, a test organism. Phytopathol. 45: 398-404
Research Articles

How to Cite

Compatibility of biocontrol agents and N fixing organisms with post emergence pre-mix herbicide-bispyribac sodium + metamifop 14 % SE. (2017). Journal of Applied and Natural Science, 9(3), 1510-1514. https://doi.org/10.31018/jans.v9i3.1393