The present study was conducted to determine inhibitory effect of plant extracts, their botanical formula-tions (BF1 and BF2) and safer fungicides against Botryosphaeria dothidea cause white rot of apple (Malus × domes-tica) during storage. Poisoned food technique was performed to evaluate the efficacy of different plant extracts, their botanical formulations and safer fungicides. Out of twelve botanicals evaluated under in vitro conditions against the white rot pathogen, leaf extract of Ocimum sanctum was found most effective among all the treatments with 54.07 per cent average inhibition in mycelial growth. Out of twelve plants evaluated for their efficacy, six effective plants Karu (Roylea elegans), Artemisia (Artemisia roxburghiana), Neem (Azadirachta indica), Bana (Vitex negundo), Tulsi (Ocimum sanctum) and Darek (Melia azedarach) were selected for making two botanical formulations (BF1 and BF2). While, BF1 was water based formulation and BF2 was cow urine based formulation. Among these botanical formulations, BF2 inhibited mycelial growth of white rot pathogen by 72.70 per cent and BF1 66.37 per cent at 100 % concentration. Whereas, among the tested fungicides, Score at 100 ppm concentration was found to be most effective with 75.01 % average inhibition in mycelial growth of the white rot pathogen.
Apple, Botanical formulation, Cow urine, Plant extracts, White rot
Arya, A. (2010). Recent advances in the management of fungal pathogens of fruit crops. In: Management of Fungal Plant Pathogens, Arya A. and Perello A. E.(eds.), Commonwealth Agricultural Bureaux International,Wallingford,UK. pp.1-25.
Auger, J., Esterio, M., Ricke, G. and Perez, I. (2004). Black dead arm and basal canker of Vitisvinifera cv. Red Globe caused by Botryosphaeria obtusa in Chile. Plant Dis., 88: 1286.
Basak, A.B., Lee, M.W. and Lee, T. S. (2002). Inhibitive activity of cow urine and cow dung against Sclerotinia sclerotiorum of cucumber. Mycobiology., 30(3): 175-179.
Carpinella, M. C., Herrero, G. G., Alonso, R. A. and Palacios, S. M. (1999). Antifungal activity of Melia azedarach fruit extract. Fitoterapia., 70: 296-298.
Chauhan, R.S. (2004). Panchagavya Therapy (Cow pathy): current status and future directions. The Indian Cow., 1: 3-7.
Falck, R. (1907). Wachtumgesetze, wachstumlotaktorchund temperature wertderholzersterenden. Myceture, 32:38-39.
Faria, T. J., Ferreira, R. S., Yassumoto, L., Desouza,J R P., Ishikawa, N. K. and Barbosa, A. D. (2006). Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi. Braz Arch Bio and Technol., 49(6): 867-871.
Fulkerson, J. F.(1960). Botryosphaeria ribis and its relation to rot of apple. Phytopathology, 50: 394-398.
Gautam, H. R.( 2011). Eco-friendly bio-formulation for fruit production. Science Reporter., 48 (6): 17.
Jones A L and Aldwinckle H S (eds.). 1990. Compendium of Apple and Pear Diseases. American Phytopathological Society, Press, St. Paul, Minnesota, USA. 100 pp.
Kaul, J. L. (1979). Studies on important postharvest diseases of apple caused by fungi and their control, Ph.D. Thesis, Himachal Pradesh Krishi Vishvavidyalaya, Solan (HP), India 214 p.
Kim, D. H. and Uhm, J. Y. (2002). Effect of application timing of ergosterol biosynthesis-inhibiting fungicides on the suppression of disease and latent infection of apple white rot caused by Botryosphaeria dothidea. J. Gen. Plant Pathol., 68(3): 237-245.
Konstantinou, S., Karaoglanidis, G. S., Bardas, G. A., Minas, I. S., Doukas, E. and Markoglou, A. N. (2011). Postharvest fruit rots of apple in Greece: pathogen incidence and relationships between fruit quality parameters, cultivar susceptibility and patulin production. Plant Dis., 95: 666-672.
Lopez-Reyes, J. G., Spadaro, D., Prelle, A., Garibaldi, A. and Gullino M. L. (2013). Efficacy of plant essential oils on postharvest control of rots caused by fungi on different stone fruits in vivo. J.Food Prot.,76 (4): 631-639.
Minocheherhomji, F. P. and Vyas, B.M. (2014). Study of the antimicrobial activity of cow urine and medicinal plant extracts on pathogenic Human microbial strains. IJAPBC., 3(4): 836-840.
Morales, A., Latorre, B. A., Piontelli, E. and Besoain, X. (2012). Botryosphaeriaceae species affecting table grape vineyards in Chile and cultivar susceptibility. Cienc. Investig. Agrar., 39 (3): 445-458.
Murugan, A. M., Shanthi, S., Arunachalam, C., Sivakumar, N., Elamathy, S. and Rajapandian, K. (2012). Study on cow urine and Pongamiapinnata Linn seed in farmyard: a natural, cost effective, ecofriendly remedy to bacterial leaf blight (BLB) of paddy. Afr. J. Biotechnol., 11(40): 9591-9598.
NHB, Gurgaon (2014). Indian Horticulture Database. National Horticulture Board, Gurgaon, Ministry of Agriculture, Government of India, 286pp.
Onyeani, C. A., Osunlaja, S. O., Oworu, O. O. and Joda, A. O. (2012). Evaluation of effect of aqueous plant extract in the control of storage fungi. I J S T R., 1(6): 76-79.
Patil, R. H. S. (2007). Antifungal potency of cow urine. Bioscience.,1: 4-5.
Pitt, W. M., Sosnowski, M. R., Taylor, A., Juan, H. R., Quirk, L. , Hackett, S., Somers, A., Steel, C. C. and Savocchia, S. (2010). Management of Botryosphaeria canker of grapevines. Australian Viticulture Practical Vineyard Management., 4(2): 52- 56.
Putterill, V. A. (1919). A new apple tree canker. S Afr J Sci., 16: 258-271.
Raj, H. and Sharma, R. L. (2013). Bioefficacy of aqueous- and cow urine based bioformulations against grey mould of strawberry. J Mycol Plant Pathol., 43(3): 319-322.
Raj, H. and Tomar, M. (2013). Use of aqueous and cow urine based plant extracts against post-harvest diseases of apple. J Mycol Plant Pathol., 43 (2): 246-249.
Sathasivam, A., Muthuselvam, M. and Rajendran, R. (2010). Antimicrobial activities of cow urine distillate against some clinical pathogens. GJ P., 4(1): 41-44.
Snowden, A. L. (1990). A color atlas of post-harvest diseases and disorders of fruits and vegetables. Vol. 1. General introduction and fruits. CRC Press, Boca Raton, FL, 302 pp.
Tomar, M. and Raj, H. (2015) Use of native local bio resources and cow urine for the effective management of post-harvest diseases of apples in northwest himalayan states of India. Journal of Plant Pathology and Microbiology.,l 6: 316.
Vincent, J. M. (1947). Distortion of fungal hypae in the presence of certain inhibitors. Nature 150: 850.
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