Insecticidal effects of aqueous extracts of wild pomegranate peel and seed (Punica granatum L.) against rose aphids, Macrosiphum rosaeformis
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Abstract
Efficacy of wild pomegranate (Punica granatum Linn.) fruit peel and seeds aqueous extract was evaluated for the control of common insect pests aphids (Macrosiphum rosaeformis) and for its influence on useful non-target predators viz., Coccinella septempunctata. Lethal doses of daru peel and seed suggested these became more toxic to aphids after 24 hr of exposure. A significant difference was obtained in percentage aphids reached to untreated leaves over peel extract treated leaves (23:57 and 23:77) and seed extract treated leaves (7:80 and 17:80) at 90 and 180 min time intervals in food choice assays. No significant effect was observed against Coccinella. LC50 values of daru peel and seed extracts along with mixture of three flavonoids (Kaempferol, Quercetin and Myricetin) were assessed at 48 hr values were 34.9, 4.7 and 0.6 mg/ml and at 72 hr these were 16.1, 0.000001 and 0.00001 mg/ml, respectively. In both the cases field bioassays showed affectivity till 7 DAT. Field data indicated that only X1(1 mg/ml) and X2 (0.1 mg/ml) concentrations of mixture were effective till 11 days after treatment. These are the first reports of the toxic effect of wild pomegranate (daru) fruit peel aqueous extract against M. rosaeformis, hence the study suggests possible usage of Punica granatum peel for the control of rose aphids.
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Keywords
Botanical insecticides, Eco-friendly agriculture, Plant extracts, Punica granatum
References
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Chebaibi, A. and Filali, F.R. (2013) Bactericidal activity and phytochemical screening of Moroccan pomegranate (Punica granatum L.) peel aqueous extract. Journal of Medicinal Plants Research. 7 (14): 887–891.
Dahham, S.S., Ali, M.N., Tabassum, H. and Khan, M. (2010) Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). American-Eurasian journal Agriculture and Environmental Science. 9 (3) : 273–281.
Devi, A., Singh, V. and Bhatt, A.B. (2011) In vitro antibacterial activity of Pomegranate and daru (wild pomegranate) against dental plague bacteria. International journal of Pharmacy and Pharmaceutical sciences. 3 (4): 182–184.
Dhembare, A.J., Deokar, M. and Kolte, P. (2011) Efficacy of some plants extracts against rose aphids, Macrosiphum rosaeformis (Davis). Journal of Experimental Zoology India. 14 (1): 325–327.
Dipak, G., Axay, P., Manodeep, C. and Jagdish, K. (2012) Phytochemical and pharmacological profile of Punica granatum. International Research Journal of Pharmacy. 3 (2): 65–68.
Ghaly, N.S., Mina, S.A., Abdel-Aziz, N.F. and Sammour, E.A. (2014) Insecticidal activity of the main flavonoids from the leaves of Kalanchoe beharensis and K. longiflora. Journal of Natural Products. 7: 196–202.
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Guddewar, M.B., Shukla, A., Chandra, R., Pandey, S. and Saini, M.L. (1994) Tabenaemontana coranaria B. Apocynacae, a potential source of botanical insecticides.Plant Protection Bulletin, 46:1-5.
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Hamouda, A.B., Mechi, A., Zarrad, K., Laarif, A. and Chaieb, I. (2015b) Disruptive effects of pomegranate, Punica granatum Linn. (Lythraceae) extracts on the feeding, digestion and morphology of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Entomology and Applied Science Letters. 2 (2): 1–6.
Iqbal, M.F., Kahloon, M.H., Nawaz, M.R. and Javid, M.J. (2011) Effectiveness of some botanical extracts on wheat aphids. The Journal of Animal and Plant Sciences. 21 (1): 114–115.
Jung, J.S. (2015) Insecticidal effects from ethanol extracts of root peel, stem peel, and fruit peel of pomegranate (Punica granatum L.) on house dust mite. International journal of bio-science and bio-technology. 7 (2): 25–36.
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Kumar, K. and Chapman, R. B. (1984) Toxicity of insecticides to cabbage aphid, Brevicoryne brassicae L. New Zealand Journal of Experimental Agriculture. 12: 55–58.
Matter, M.M., Marei, S.S., Moawad, S.M. and Elgengaihi, S. (1993) The relation of Aphis gossypii and its predator Coccinella septumpunctata to some plant extracts. Bulletin of Forestry of Agriculture. 44: 417-432.
Mesbah, H.A., Saad, A.S., Mourad, A.K., Taman, F.A. and Mohamed, I.B. (2007) Joint action of quercetin with four insecticides on the cotton leaf-worm larvae, Spodoptera littoralis Boisd. (Lep. :Noctuidae) in Egypt. Communications in agricultural and applied biological sciences. 72 (3): 445–57.
Mir, M.M., Umar, I., Mir, S.A., Rehman, M.U., Rather, G.H. and Banday, S.A., 2012. Quality evaluation of pomegranate crop – a review. International Journal of Agriculture and Biology. 14: 658–667.
Mitchell, M.J., Daniel, P.K., John, R.C. and Stan, L.S. (1993) Effects of plant flavonoids and other allelochemicals on insect cytochrome P-450 dependent steroid hydroxylase activity. Insect Biochemistry and Molecular Biology. 23: 65–71.
Parmar, C. and Kaushal, M.K. (1982) Wild fruits of the sub-Himalayan region. Kalyani Publishers, Ludhiana. pp 136.
Parmar, C. (2013) Encyclopedia of the edible fruits of the world. Fruitipedia.
Rahimi, H.R., Arastoo, M. and Ostad, S.N. (2012) A comprehensive review of Punica granatum (pomegranate) properties in toxicological, pharmacological, cellular and molecular biology researches. Iranian Journal of pharmaceutical Research. 11 (2): 385–400.
Sarmah, M., Rahmam, A., Phukan, A.K. and Gurusubramanian, G. (2009) Effect of aqueous plant extracts on tea red spider mite, Oligonychus coffeae, Nietner (Tertranychidae acarina) and Stethorus gilivifrons Mulsant. African Journal of Biotechnology. 8 (3) : 417–423.
Sohail, A., Hamid, F.S., Waheeb, A., Ahmad, N., Aslam, N., Zaman, O., Ahmed, F. and Isman, S. (2012) Efficacy of different botanical materials against aphid, Toxoptera aurantiion on Tea (Camellia sinensis L.) cutting under high shade nursery. Journal of Material and Environmental Science. 3 (6): 1065–1070.
Upasani, S.M., Kotkar, H.M., Mendki, P.S. and Maheshwari, U.L. (2003) Partial characterization and insecticidal properties of Ricinus communis L. foliage flavonoids. Pest management science. 59 (12): 1349–1354.
Xiaofeng, M., Liping, Z. and Bingyong, G. (1999) Studies on the metabolites of actinomyces SIPI-9764. Chinese Journal of Antibiotics. 24: 173–174.
Zhao, X., Yuan, Z., Fang, Y., Yin, Y and Feng, L. (2014) Flavonols and flavones changes in pomegranate (Punica granatum L.) fruit peel during fruit development. Journal of Agricultural Science and Technology.16:1649–1659.
Ateyyat, M., Abu-Romman, S., Abu- Darwish, M. and Ghabeish, I. (2012) Impact of flavonoids against woolly apple aphid, Eriosoma lanigerum and its sole parasitoid, Aphelinus mali. Journal of Agricultural Science. 4: 2.
Chebaibi, A. and Filali, F.R. (2013) Bactericidal activity and phytochemical screening of Moroccan pomegranate (Punica granatum L.) peel aqueous extract. Journal of Medicinal Plants Research. 7 (14): 887–891.
Dahham, S.S., Ali, M.N., Tabassum, H. and Khan, M. (2010) Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). American-Eurasian journal Agriculture and Environmental Science. 9 (3) : 273–281.
Devi, A., Singh, V. and Bhatt, A.B. (2011) In vitro antibacterial activity of Pomegranate and daru (wild pomegranate) against dental plague bacteria. International journal of Pharmacy and Pharmaceutical sciences. 3 (4): 182–184.
Dhembare, A.J., Deokar, M. and Kolte, P. (2011) Efficacy of some plants extracts against rose aphids, Macrosiphum rosaeformis (Davis). Journal of Experimental Zoology India. 14 (1): 325–327.
Dipak, G., Axay, P., Manodeep, C. and Jagdish, K. (2012) Phytochemical and pharmacological profile of Punica granatum. International Research Journal of Pharmacy. 3 (2): 65–68.
Ghaly, N.S., Mina, S.A., Abdel-Aziz, N.F. and Sammour, E.A. (2014) Insecticidal activity of the main flavonoids from the leaves of Kalanchoe beharensis and K. longiflora. Journal of Natural Products. 7: 196–202.
Golawaska, S. and Lukasik, I. (2012) Antifeedent activity of luteolin and genestein against the pea aphid, Acyrthosiphon pisum. Journal of Pest Science. 85 (4): 443–450.
Guddewar, M.B., Shukla, A., Chandra, R., Pandey, S. and Saini, M.L. (1994) Tabenaemontana coranaria B. Apocynacae, a potential source of botanical insecticides.Plant Protection Bulletin, 46:1-5.
Hamouda, A.B, Chaieb, I., Daami-Remadi, M. and Laarif, A. (2015a) In vitro evaluation of insecticidal and antifungal potencies of fruit peel extracts of pomegranate (Punica granatum). Central European journal of experimental biology. 4 (1): 11–15.
Hamouda, A.B., Mechi, A., Zarrad, K., Laarif, A. and Chaieb, I. (2015b) Disruptive effects of pomegranate, Punica granatum Linn. (Lythraceae) extracts on the feeding, digestion and morphology of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Entomology and Applied Science Letters. 2 (2): 1–6.
Iqbal, M.F., Kahloon, M.H., Nawaz, M.R. and Javid, M.J. (2011) Effectiveness of some botanical extracts on wheat aphids. The Journal of Animal and Plant Sciences. 21 (1): 114–115.
Jung, J.S. (2015) Insecticidal effects from ethanol extracts of root peel, stem peel, and fruit peel of pomegranate (Punica granatum L.) on house dust mite. International journal of bio-science and bio-technology. 7 (2): 25–36.
Kotkar, E., Mendki, P.S., Sadan, S.V., Jha, S.R. and Maheshwari, V.L. (2002) Antimicrobial and pesticidal activity of partially purified flavonoids of Annona squamosa. Pest Management Science. 58: 33–37.
Kumar, K. and Chapman, R. B. (1984) Toxicity of insecticides to cabbage aphid, Brevicoryne brassicae L. New Zealand Journal of Experimental Agriculture. 12: 55–58.
Matter, M.M., Marei, S.S., Moawad, S.M. and Elgengaihi, S. (1993) The relation of Aphis gossypii and its predator Coccinella septumpunctata to some plant extracts. Bulletin of Forestry of Agriculture. 44: 417-432.
Mesbah, H.A., Saad, A.S., Mourad, A.K., Taman, F.A. and Mohamed, I.B. (2007) Joint action of quercetin with four insecticides on the cotton leaf-worm larvae, Spodoptera littoralis Boisd. (Lep. :Noctuidae) in Egypt. Communications in agricultural and applied biological sciences. 72 (3): 445–57.
Mir, M.M., Umar, I., Mir, S.A., Rehman, M.U., Rather, G.H. and Banday, S.A., 2012. Quality evaluation of pomegranate crop – a review. International Journal of Agriculture and Biology. 14: 658–667.
Mitchell, M.J., Daniel, P.K., John, R.C. and Stan, L.S. (1993) Effects of plant flavonoids and other allelochemicals on insect cytochrome P-450 dependent steroid hydroxylase activity. Insect Biochemistry and Molecular Biology. 23: 65–71.
Parmar, C. and Kaushal, M.K. (1982) Wild fruits of the sub-Himalayan region. Kalyani Publishers, Ludhiana. pp 136.
Parmar, C. (2013) Encyclopedia of the edible fruits of the world. Fruitipedia.
Rahimi, H.R., Arastoo, M. and Ostad, S.N. (2012) A comprehensive review of Punica granatum (pomegranate) properties in toxicological, pharmacological, cellular and molecular biology researches. Iranian Journal of pharmaceutical Research. 11 (2): 385–400.
Sarmah, M., Rahmam, A., Phukan, A.K. and Gurusubramanian, G. (2009) Effect of aqueous plant extracts on tea red spider mite, Oligonychus coffeae, Nietner (Tertranychidae acarina) and Stethorus gilivifrons Mulsant. African Journal of Biotechnology. 8 (3) : 417–423.
Sohail, A., Hamid, F.S., Waheeb, A., Ahmad, N., Aslam, N., Zaman, O., Ahmed, F. and Isman, S. (2012) Efficacy of different botanical materials against aphid, Toxoptera aurantiion on Tea (Camellia sinensis L.) cutting under high shade nursery. Journal of Material and Environmental Science. 3 (6): 1065–1070.
Upasani, S.M., Kotkar, H.M., Mendki, P.S. and Maheshwari, U.L. (2003) Partial characterization and insecticidal properties of Ricinus communis L. foliage flavonoids. Pest management science. 59 (12): 1349–1354.
Xiaofeng, M., Liping, Z. and Bingyong, G. (1999) Studies on the metabolites of actinomyces SIPI-9764. Chinese Journal of Antibiotics. 24: 173–174.
Zhao, X., Yuan, Z., Fang, Y., Yin, Y and Feng, L. (2014) Flavonols and flavones changes in pomegranate (Punica granatum L.) fruit peel during fruit development. Journal of Agricultural Science and Technology.16:1649–1659.
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Insecticidal effects of aqueous extracts of wild pomegranate peel and seed (Punica granatum L.) against rose aphids, Macrosiphum rosaeformis. (2017). Journal of Applied and Natural Science, 9(3), 1397-1405. https://doi.org/10.31018/jans.v9i3.1374
