Sonia Sood Sanjay Jambhulkar Yudhvir Singh Nivedita Gupta Saloni Sharma


An investigation entitled “Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum)” was conducted during kharif (summer-rainy season) 2012 and 2013 at Experimental farm of the Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, H.P., India. Healthy seeds of California Wonder of bell pepper were exposed to physical mutagen Gamma rays using 60CO as a source of radiation at Mutation Breeding Centre, Department of Biotechnology, BARC Trombay, Mumbai and chemical mutagen EMS (Ethyl Methane Sulphonate) to obtain the spectrum and frequency of chlorophyll mutations in M2 generation. The M1 generation was produced from these mutagen treated seeds. Several unique and interesting chlorophyll and viable mutants were obtained in M2 generation. In M2 generation, gamma rays induced higher proportion of chlorophyll mutants then EMS. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses/concentrations. Four different types of chlorophyll mutants namely xantha, yellow xantha, chlorina and viridis were induced. Out of these mutants, chlorina and viridis were most frequent and were produced even in lower doses/concentrations while yellow xantha was least frequent and produced only in higher doses. The highest frequency of chlorophyll mutations (18.8 %) was reported in the 22 kR of gamma dose, while the lowest (0.80 %) frequency of chlorophyll mutations was found in the treatment of 1.0 % EMS. There was a dose dependent increase in the spectrum and frequency of chlorophyll mutations. These chlorophyll mutants induced by gamma radiation and EMS could be used in mutation breeding programme for inducing viable mutations for improvement of bell pepper varieties.


Download data is not yet available.


Metrics Loading ...




Bell pepper, Chlorophyll mutants, Spectrum, Frequency, Gamma rays, Ethyl methane sulphonate

Alcantra, T.P., Bosland, P.W. and Smith, D.W. (1996). Ethyl methane sulfonate induced mutagenesis of Capsicum annuum. J. Heredity, 87: 239-241
Ambli, K., Mullainathan, L. and Natarajan, S. (2016). Mutagenic effectiveness and efficiency of gamma rays and ethyl methane sulphonatein pearl millet (Pennisetumtyphoides (Burn.F.) Stapf. and C.E.Hubb.) Var.CO (cu)-9. Academia J. Agri. Res. 4(2): 41-44
Bind, D., Dwivedi, V. K. and Singh, S. K., (2016). Induction of chlorophyll mutations through physical and chemical mutagenesis in cowpea [Vignaunguiculata (L.) Walp.]. Int. J. Adv. Res., 4(2): 49-53
Bosland P. W., Bailey A. L. and Iglesias-Olivas. (1996). Capsicum pepper varieties and classifaction. New Maxico Cooerative Extension Service Circal, Las Cruces New Maxico. P. 530
Firdose, K., Pawar, N. and Dixit, G. (2011). Induced chlorophyll mutations in Delphinium. J. Appl. Horti., 13(1): 18-24
Gaur, L. B., Singh, S. P. and Srivastava, K. (2013). Frequency and spectrum of chlorophyll mutation in chilli (Capsicum annuum L.). Int. J. Plant Sci., 8(1):
Gustafson, A., (1940). The mutation system of chlorophyll apparatus. Lund InivArskr. 36: 1-40.Hiscox, J.D. and Israelstam, G.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian J. Bot., 57:1332-1334
Ignacimuthu, S. and Babu, C. R. (1988). Radio sensitivity of the wild and cultivated urd and mung beans. Indian J. Gen. Plant Breed, 48(3): 331-342
Kumar, A. G., Ponnuswami, V. and Sundar, S. T. B. (2012). Effect of induced chlorophyll mutation, mutagenic efficiency and effectiveness of gamma rays and EMS in paprika (Capsicum annuum L.) cv. BydagiKaddi. Indian J.Horti., 69(1): 60-64
Manju, P., Mercy, S. T. and Nair, V. G. (1983). Induction of variability in horse gram (Vignaunguiculata) with EMS and Gamma rays. Legume Res., 6: 21-28
Nascimento, K. S., Rego, M. M., Nascimento, A. M. and Rego, A. R. (2015). Ethyl methane sulphonate in the generation of genetic variability in Capsicum. Acta Hort., 1087: 249-254
Ojiewo, C. O., Agong, S. G. and Masuda, M. (2006). Biological effect of carbon-ion beam on mutagenesis and a novel season-dependent floral homeotic mutant in Solanum villosum Miller. J. Horti. Sci. Biotech., 81: 559-564
Patil, B. M. and Rane, G. M. (2015). Gamma radiation induced chlorophyll mutations in cluster bean (Cyamopsistetragonoloba (L.) Taub) Var. NCB-12. Int. J. Allied Prac. Res, Review, 2(2): 75-85
Rodrigues, R., Gonçalves, L. S. A., Bento, C. S., Sudre, C. P., Robaina, R. R. and Junior, A. A. (2012). Combining ability and heterosis for agronomic traits in chili pepper. Horticultura Brasileira, 30: 226-233
Sridevi, A. and Mullainathan, L. (2011). Physical and chemical mutagenesis for improvement of chilli (Capsicum annum L.). World Appl. Sci. J., 15 (1): 108-113
Vadluri, R., Thupurani, M. K., Jogam, P. and Abbagani, S. (2016). Evaluation of morphological and cytological variations induced by Ethyl Methane Sulphonate in Capsicum annum.L. Int. J. Adv. Res., http://dx.doi.org/10.21474/IJAR01/2350
Waghmare, V. N. and Mehra, R. B. (2001). Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrussativus L. Indian J. Gen., 58: 465-474
Walles, B., (1973). Plastid structures and mutations. In: Structure and Function of Chloroplasts, Gibba M. (ed), Springer Verlag New York, pp. 51-58.
Wildman, S.G., (1973). An approach towards ascertaining the function of chloroplast DNA in tobacco plants. In: Autonomy of Bio-genesis, Linnae N.K. and Smillie A.W. (eds), New York AE Rube Co
Citation Format
How to Cite
Sood, S., Jambhulkar, S., Singh, Y., Gupta, N., & Sharma, S. (2017). Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum). Journal of Applied and Natural Science, 9(2), 1085–1089. https://doi.org/10.31018/jans.v9i2.1326
More Citation Formats:
Research Articles