##plugins.themes.bootstrap3.article.main##

Nisha Kumari Ram Avtar Bunty Sharma Babita Rani Veena Jain R.K. Sheoran

Abstract

The present investigation was carried out to evaluate the nutritional potential of five different Indian mustard genotypes. Fatty acid composition was determined in the oil, whereas seed meal was analyzed for limiting amino acids (tryptophan and methionine), protein content, glucosinolate content and antioxidant potential (DPPH free radical scavenging activity, total antioxidant activity and iron chelating activity). The monounsaturated fatty acids (MUFA) were found to be maximum in RH 0749 (58.70 %) followed by RH (OE) 0801 (48.91 %), JM 6011 (47.03 %), EC 597328 and EC 597340 (45.77 %). Polyunsaturated fatty acids (PUFA) were observed maximum in EC 597340 (47.45 %).Glucosinolate content ranged from 42.80 (EC 597328) to 79.79 ?mole/g defatted seed meal (EC 597340). The methanolic seed meal extract exhibited a concentration dependent elimination of DPPH free radicals. All the five genotypes showed about 50 % inhibition in 3.0 mg of dry seed meal. The highest total antioxidant activity (20.41mg/g) and metal ion chelating activity (32.58 %) was observed in RH 0749. Protein content varied from 33.57 [RH (OE) 0801] to 38.01 % (RH 0749). Maximum methionine and tryptophan content were recorded in RH 0749 (0.99 and 1.01 g/100g protein, respectively). Thus, RH 0749 was observed as a potent variety in terms of total antioxidant activity, metal ion chelating activity, protein content, methionine and tryptophan content.

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Antioxidant, Fatty acids, Glucosinolates, Methoinine, Protein

References
Andlauer, W. and Furst, P. (1998). Antioxidative power of phytochemicals with special reference to cereales. Cereal Foods World, 43: 356-359
Bala, M. and Singh, M. (2012). Non-destructive estimation of total phenol and crude fiber content in intact seeds of rapeseed-mustard using FTNIR. Industrial Crops and Products, 42: 357-362
Bala, M., Kumar, S. and Singh, L. (2011). Antioxidant potential of rapeseed mustard seed meal extracts. Indian journal of Agricultural Biochemistry, 24: 154-174
Bell, J. M. (1984). Nutrients and toxicants in rapeseed meal: a review. Journal of Animal Science, 58: 996-1010
Bille, N., Eggum, B. O., Jacobsen, I., Olsen, O. And Sørensen, H. (1983). The effects of processing on antinutritional rape constituents and the nutritive value of double low rapeseed meal, Zeitschr. Tierphysiol Tierernährungu.Futtermittelkd, 49: 148-163
Chauhan, J. S., Kumar, S., Singh, K. H., Meena, S. S. and Meena, M. L. (2010). Oil and Seed Meal Quality Indices of Indian Rapeseed-Mustard Varieties. Journal of Plant Biochemistry & Biotechnology, 19(1): 83-86
Dua, A., Chander, S., Aggrawal, S. and Mahajan, R. (2014). Antioxidants from defatted Indian mustard (Brassica juncea) protect biomolecules against in vitro oxidation. Physiology and Molecular Biology of Plants, 20:539-543
Halliwell, B. (2010). Free radicals and antioxidants-quo vadis? Trends in Pharmacological Sciences, 32:125-130
Horn, M. J., Jones, D. B. and Blum, A. E. (1946). Colorimetric determination of methionine in proteins and foods. Journal of Biological Chemistry, 116:313–320
Hsu, C. L., Chen, W., Weng, Y. M. and Tseng, C. Y. (2003). Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods. Food Chemistry, 83:
85-92
Ishtiaque, S. Khan, N., Siddiqui, M. A, Siddiqi, R. and Naz, S. (2013). Antioxidant potential of the extracts, fractions and oils derived from oilseeds. Antioxidants, 2: 246-256
Kaushik, N. And Agnihotri A. (2000). GLC analysis of Indian rapeseed-mustard to study the variability of fatty acid composition. Biochemical Society Transactions, 28(6): 581-583
Kumari, N., Avtar, R., Sharma, B. and Thakral, N. (2016) Antioxidant potential of seed meal of different Indian mustard genotypes. Journal of Oilseeds Brassica, 7(1): 63-67
Prieto, P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1. Analytical Biochemistry, 269: 337-341
Priyamedha, S., Singh, B. K., Ram, B., Kumar, A., Singh, V. V., Meena, M. L. and Singh, D. (2014). Development and evaluation of double low quality lines in indian mustard (Brassica juncea L. Czern & Coss). SABRAO Journal of Breeding and Genetics, 46 (2): 274-283
Saxena, S. N., Karwa, S., Saxena, R., Sharma, T., Sharma, Y. K., Kakani, R. K. and Anwer, M. M (2011). Analysis of antioxidant phenolics and flavonoid content of fenugreek seed extract. International Journal of seed Spices, 1: 38-43
Singh, B. K., Bala, M. and Rai, P. K. (2014). Fatty acid composition and seed meal characteristics of Brassica and allied genera. National Academy Science Letter, 37: 219-226
Spies, J. R. and Chambers, D. C. (1949). Chemical determination of tryptophan in protein. Analytical Chemistry, 21: 1249-1265
Sutariya, D. A., Patel, K. M., Bhadauria, H. S, Vaghela, P. O., Prajapati, D. B., Parmar, S. K. (2011). Genetic diversity for quality traits in Indian mustard (Brassica juncea L.). Journal of Oilseed Brassica, 2(1): 44-47
Vasudev, S., Yadava, D. K., Malik, D., Tanwar, R. S. and. Prabhu, K. V. (2008). A simplified method for preparation of fatty acid methyl esters of Brassica oil. ?Indian Journal of Genetics and Plant Breeding, 68(4): 456-458
Verma, K. C. and Baigh, M. A. (2012). Response of phosphorous and molybdenum on yield and quality attributing characters of Indian mustard (Brassica juncea L. Czern & Coss). The Bioscan, 7(3): 437-440
Yen, G. C. and Duh, P. D. (1993). Antioxidative properties of methanolic extract from peanut hulls. Journal of American Oil Chemists Society, 4: 383-386
Section
Research Articles

How to Cite

Biochemical assessment of nutritional status in Indian mustard. (2017). Journal of Applied and Natural Science, 9(2), 1068-1071. https://doi.org/10.31018/jans.v9i2.1322