Naresh Pratap Singh Vaishali Vaishali


To feed the ever growing world population, the demand of food supply must be increased by 70 % of major cereal crops like wheat, rice etc. It was predicted that the detrimental effect of abiotic stresses like drought, heat, salt etc. on yield would be decreased by genetic improvement in terms of photosynthetic response, long green leaf duration and delayed leaf senescence. ‘Stay green’ is a vital trait of all crops which is directly associated with the capacity of the plant to maintain CO2 assimilation, photosynthesis and chlorophyll content. The present study was conducted to develop the stay green mutants genotype by using 1.5 % Ethyl Methane Sulphonate (EMS). The variety K 7410 showed highest leaf area 37.34 cm2, seeds per spike 65.47, 1000 grain weight 62.03 g after treatment of EMS among morphological characters observed. Among physiological characters of wheat variety Sonalik showed lowest RWC (21.48 %), HD 2135 showed lowest chlorophyll content (33.53 µg/cm2) and C 306 showed lowest photosynthetic rate (15.05 µmol/m2sec) after treatment of EMS. But varieties K 7410, VL 401 and RAJ 3765 varieties showed higher value of physiological characters after the treatment. The results suggested that the stay green trait had been developed by mutation (EMS) in these three wheat varieties and they can exhibit better tolerance under abiotic stress conditions like drought, high temperature. Such results would prove useful for further research and crop management in stress affected areas or under unfavourable climatic conditions.


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Chlorophyll, Morphological, Photosynthesis, RWC, Stay green, Wheat

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Singh, N. P., & Vaishali, V. (2017). Effect of EMS on morpho-physiological characters of wheat in reference to stay green trait. Journal of Applied and Natural Science, 9(2), 1026–1031. https://doi.org/10.31018/jans.v9i2.1316
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