Genetic diversity in tomato (Solanum lycopersicum L.) genotypes revealed by simple sequence repeats (SSR) markers
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Abstract
Twenty five tomato (Solanum lycopersicum L.) genotypes were subjected to genetic diversity analysis using twenty SSR markers. Out of 20 markers used, 14 SSRs were polymorphic and a total numbers of 22 SSR alleles were generated by 14 SSR markers, out of which 19 were polymorphic and 3 were monomorphic, with an average of 1.57 alleles per locus. The range of amplified products was 100-400bp approximately. Jaccard’s similarity coefficient varied from 0.65 between germplasm EC519821 and CO-3 to a maximum of 1.0 between genotypes EC519769 and DARL-66, with an average value of 0.83. Cluster analysis based on Jaccard’s similarity coefficient using the unweighted pair-group method with arithmetic mean (UPGMA) revealed 2 distinct clusters, A and B, comprising 1 and 24 genotypes respectively and at 75 and 78 per cent similarity, respectively. The genotypes which showed similar morphological and genetic trends were grouped more or less together in both these cases were a few. Cluster A comprised most diverse germplasm (EC519821)belongs to pimpinellifolium wild species with similarity coefficient 0.65% and differentiated with other cultivated species.Cherry Tomato and Cherry-2 were trends in similar cluster similar with approximately 96% similarity.SSR markers were able in in differentiating the genotypes based on morphologically and genotypically.However, the grouping of 25 genotypes were independently of geographic distribution.The genetic distance information found in this study might be helpful to breeder for planning among these genotypes.
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Genetic diversity, accard’s similarity coefficient, SSR, Tomato genotypes
Ansari, A., M.(2015). Molecular markers in vegetable improvement Horticultural Biotechnology Research.1(1):5-10
Barone, A., Chiusano, M. L., Ercolano, M. R., Giuliano, G., Grandillo, S., &Frusciante, L. (2008). Structural and Functional Genomics of Tomato. International Journal of Plant Genomics, 2008, 820274. http://doi.org/10.1155/2008/820274
Benor,S.,Zhang, M. Y., Wang ,Z. F. and Zhang,H. S. ( 2008) Assessment of genetic variation in tomato (SolanumlycopersicumL.) inbred lines using SSR molecular markers Journal of Genetic Genomics, 35: 373-379
Bredemeijer, G.M.M., Arens, P. and Wouters,D.( 1998) The use of semiautomated fluorescent microsatellite analysis for tomato cultivar identification. Theoretical and Applied Genetics, 97:584?590
Caguiat X.G.I. and Hautea D.M. (2014).Genetic diversity analysis of eggplant (Solanum melongenaL.) and related wild species in the philippines using morphological and ssr markers SABRAO Journalof Breeding and Genetics, 46 (2) 183-201
Corrado, G. P., Piffanelli, M., Caramante, M., Coppola and Rao R. (2013): SNP genotyping reveals genetic diversity between cultivated landraces and contemporary varieties of tomato. BMC Genomics, 14: 835. doi:10.1186/1471-2164-14-835.
Dhaliwal, M. S., Singh, M., Singh, K. and Cheema, D.S.(2011).Genetic diversity analysis and DNA fingerprinting of elite genetic stock of tomato using SSR marker. Indian Journal Genetics, 71: 341- 348
Doyle ,J. J. and Doyle , J. L. (1990) Isolation of plant DNA from fresh tissues. Focus, 12: 13-15
Edris, S, S. Abo-Aba, M.M. Algandaby, A.M. Ramadan, N.O. Gadalla, M.A. AL-Kordy, J.S.M. Sabir, F.M. EL- DomyatI, A.M. ALZhairy and A. Bahieldin (2014): Molecular characterization of tomato cultivars grown in Saudi Arabia and differing in earliness of fruit development as revealed by AFLP and ISSR. Life Science Journal, 11 (8): 602–612
El-Awady, M., El-Tarras, A.., A .E. and Hassan, M. M.(2012) Genetic diversity and DNA fingerprint study in tomato (Solanum lycopersicumL.) cultivars grown in Egypt using simple sequence repeats (SSR) markers. African Journal of Biotechnology, 1(1):16233-16240
Idrees M. and Irshad M.(2014).Molecular markers in plants for analysis of genetic diversity: a review. European academic research, (2) 1513-1540
Ollitrault, F., Terol, J., Pina J. A., Navarro L., Talon M. and Ollitrault, P.(2010).Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus.American Journal of Botany, 97: 124–129
Rohlf, F.J.(2002).NT-SYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.1d. Exter Software: Setauket, NY.
Russel,J R., Fuller, J.D., Macaulay, M., Hatz, B. G., jahoo, A., Powell, W. and Waugh, R.(1997). Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics, 95:714-22
Sanghani, A.O. and Mandavia, M. K. (2013). Characterization of tomato (Lycopersiconlycopersicum Mill.) genotypes through RAPDs, ISSRs, and SSRs marker. Indian Journal ofAgricuture Biochemistry, 26(2): 141-147
Shirasawa, K. and Hirakawa, H. (2013) DNA marker applications to molecular genetics and genomics in tomato. Breeding Science, 63(1):21-30. doi:10.1270/jsbbs.63.21.
Smith ,J.S.C., Chin, E. C. L. , Shu, H., Smith, O.S. and Wall, S. J.(1997).An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.) comparision with data from RFLPs and pedigree. Theoretical and Applied Genetics, 95: 163-173
Zhang, S., Tang,C., Zhao, Q.,Li,J., Yang,L.,Qie,L.,Fan, X.,Li,L.,Zhang,N.,Zhao,M.,Liu,X.,Chai, Y.,Zhang, X.,Wang, H.,Li,Y.,Li, W.,Zhi, H.,Jia, G. and Diao,X. (2014) Development of highly polymorphic simple sequence repeat markers using genome-wide microsatellite variant analysis in Foxtail millet [Setariaitalica (L.) P. Beauv.]. BMC Genomics, 15:78. doi:10.1186/1471-2164-15-78.
Zhou, R., Wu, Z., Cao, X. and Jiang, F.L. (2015).Genetic diversity of cultivated and wild tomatoes revealed by morphological traits and SSR markers.Genet. Mol. Res., 14 (4): 13868-13879
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