Piperine content variation in different Piper longum germplasms of North East India determined through RP-HPLC method
##plugins.themes.bootstrap3.article.main##
Abstract
The present experiment was conducted at Assam Agricultural University, Jorhat with ten accessions including check variety of Piper Longum germplasm collected from different states of North Eastern region during 2013-14 and 2014-15.The piperine, a major alkaloid used in different therapeutic treatment, per cent content was determined from dried plant materials. Deionised water was used throughout the experiment and the chromatographic separation was carried out in an isocratic elution mode on RP-18 column with 5?m particle size, 4.6mm internal diameter and 250mm length. The mobile phase was a mixture of methanol and water (80: 20). The solvent flow rate was 1.0 ml/minwith injection volume 20 µL. The photo diode array detector wavelength was set at 342 nm for the identification of piperine in all extracts. The per cent piperine content in extract was found to be 42.36 per cent in germplasm PLJ-30.The highest per cent piperine content in fruit was recorded in PLJ-11(7.85%) followed by germplasm PLJ-9 (7.64) and significantly superior over check variety“Viswam”(5.15%). The descending order of piperine content in fruits among germplasm was PLJ-11?PLJ-09?PLJ-30?PLJ-17? PLJ-03? PLJ-19? check variety?PLJ-01? PLJ-20 and PLJ-16. It can be revealed from the present experiment that piper germplasm with high piperine content has a great scope for commercial cultivation as alkaloid piperine has high demand in pharmaceutical use.
##plugins.themes.bootstrap3.article.details##
##plugins.themes.bootstrap3.article.details##
Northeast India, Phytochemical, Piper longum, Piperine, RP-HPLC method
Atal, N. and Bedi, K.L. (2010). Bioenhancers: Revolutionary concept to market. J AyurInteg Med., 1(2): 96-99
Choudhury, P. R., Choudhury, M. D.,Ningthoujam, S. S., Das, D., Nath, D. and Talukdar, A. D (2012). Ethnomedicinal plants used by traditional healers of North Tripura district, Tripura, North East India. Journal of Ethnopharmacology, 166: 135-148
Chauhan, K., Solanki, R., Patel, A., Macwan, C. and Patel, M. (2011).Phytochemical and Therapeutic potential of Piper longumLinn.A review.IJRAP, 2(1): 157-161
Deepthi, S.P.R.,Junise, V.,Shibin, P., Senthila, S. and Rajesh, R.S. (2012).Isolation, identification and antimycobacterial evaluation of piperine from Piper longum. Der Pharmacia Lettre, 4: 863-868
Hamrapurkar, P.D., Jadhav, K. and Zine, S. (2011). Quantitative estimation of piperine in Piper nigrumand Piper longumusing high performance thin layer chromatography, J Applied Pharm Sci., 01 (03): 117-120
James, V. (1999).Piperine: the treasured alkaloid in Piper nigrum. Spice India, 12: 9-12
Kanaki, N., Dave, M., Path, H. and Rajani, M. (2008).A rapid method for isolation of piperine from the fruits of Piper nigrumLinn.JNAT, 62: 281-283
Kato, M.J. and Furlan, M. (2007). Chemistry and evolution of the Piperaceae, Pure Appl. Chem., 79(4): 529–538
Kumar, V., Shriram, V. and Mulla, J. (2013).Antibiotic resistance reversal of multiple drug resistant bacteria using Piper longumfruit extract.J App Pharma Sci., 3(03):112-116
Manoj, P., Soniya, E. V., Banerjee, N. S. and Ravichandran, P. (2004). Recent studies on well known spice Piper longum Linn. Natural Product Radiance, 3(4): 22-29
Madhavi, B.B., Nath, A.R.,Banji, D., Madhu, M.N., Ramalingam, R. and Swetha D. (2009).Extraction, identification, formulation and evaluation of piperine in alginate beads.International Journal of Pharmacy and Pharmaceutical Sciences, 1: 156-161
Menghani, E., Jain, S.C. and Jain, R. (2010).Scientific validation of Piper nigrumby HPLC and anti-oxidative assay markers.Asian J Biotech, 2: 133-138
Mishra, P. (2010). Isolation, spectroscopic characterization and computational modeling of chemical constituents of Piper longumnatural product.Int J Pharm Sci Review Res., 2(2): 78-86
Nair, K.K.N. (2000). Manual of nonwood forest produce plants of Kerala. Kerala Forest Research Institute, Kerala, 268–270
Okwute, S.K. and Egharevba, H.O. (2013). Piperine-type amides: Review of the chemical and biological characteristics. Int J Chem., 5(3) ISSN 1916-9698 E-ISSN 1916-9701
Rami, E., Sipai, S. and Patel, I. (2013). Studies on qualitative and quantitative phytochemical, analysis of Piper longumLinn, Int J Pharma Bio Sci., 4(3): (B) 1381 – 1388
Saraf, A. and Saraf, A. (2013).Chemical fingerprint profile and antibacterial studies on Piper longumLinn.Int. Conf. Exhib. Trad. Alter. Med.
Sharma, V.,Renuka, K.,Palak, V.,Harisha, C.R. and Prajapati, P.K. (2012).Pharmacognostical and phytochemical study of Piper longumL.andPiper retrofractumVAHL.
Siddique, B. S., Begum, S., Gulzar, T.and Farhat, N. F.(1997). An amide from fruits of Piper nigrum.Phytochemistry, 45: 1617-1619
Storh, R. J., Xiao, P. G. and Bauer, R.(2001). Constituents of Chinese Piper species and their inhibitory activity on prostaglandin and leukotriene biosynthesis in vitro.J of Ethnapharmacology, 75(2): 133-139
Sunila, E.S. and Kuttan, G. (2004) Immunomodulatory and antitumor activity of Piper longumLinn and piperine, 90(2-3): 339-346
Swapna, P.R.D.,Junise, V.,Shibin, P., Senthila, S. and Rajesh, R.S. (2012).Isolation, identification and antimycobacterial evaluation of piperine from Piper longum. Der Pharmacia Lettre, 4(3): 863-868
Tapsell, L.C., Hemphill, I.,Cobiac, L., Patch, C.S., Sullivan, D.R.,Fenech, M.,Roodenrys, S., Keogh, J.B., Clifton, P.M., Williams, P.G., Fazio, V.A. and Inge, K.E. (2006). Health benefits of herbs and spices: the past, the present, the future. Med. J. Aust.,185(4): S4–24
Zaveri, M.,Khandhar, A., Patel, S. and Patel, A. (2010). Chemistry and pharmacology of Piper longumLin., 5(1): 67-76
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
