Shasta Kalra Gurinder Kaur Sangha


The liver and kidney of female Bandicota bengalensis and Tatera indica rats collected from Bathinda region of south west region of Punjab were used to investigate the toxic effects of these pesticides. Levels of total proteins decreased in all the rats collected from study area. Activity levels of different OS parameters: catalase (CAT), superoxide dismutase (SOD), glutathione transferase (GST), glutathione reductase (GR) and glutathione peroxidise (GPx) were differentially altered and the product of oxidation namely, malondialdehyde increased in liver (11.05±0.18, 9.02±0.26 in control rats increased to 11.60±0.10, 9.43±2.58 in study area B. bengalensis and T. indica rats respectively) and kidney (10.03±0.44, 8.68±0.24 in control rats increased to 10.18±0.72, 8.92±4.34 in study area B. bengalensis and T. indica rats respectively) of rats collected from study area as compared to control rats. Histomorphological studies further revealed number of abnormalities as infiltration, vacuolization, enlarged sinusoids and necrosis in liver of Bathinda rats, while renal histo architecture of kidney showed high degeneration of glomeruli. The results infer that environmental contaminants mainly pesticides leads to number of pathophysiological conditions in the liver and kidney and for altering antioxidant defence system in rats inhabiting south- west region of Punjab.


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Antioxidants, Environmental contaminants, Oxidative stress, Pesticides, Proteins

Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S. and
Rezaiee, A. (2004). Pesticides and oxidative stress: a review. Medical Sci. Monitor, 10(6): 141-147
Aebi, H. (1983). Catalase In: Bergmeyer H U and Weinheim (ed) Methods of Enzymatic Analysis. Pp. 227-82.
Academic Press.
Bhushan, B., Saxena, P. N. and Saxena, N. (2013). Biochemical and histological changes in rat liver caused by
cypermethrin and beta-cyfluthrin. Arh. Hig. Rada Toksikol., 64: 57–67
Carlberg, I. and Mannervik, B. (1985). Glutathione
reductase. Methods Enzymol., 113:484-90
Chattopadhyay, S., Podder, S., Agarwal, S. and Bhattacharya, S. (2011). Fluoride-induced histopathology and synthesis of stress protein in liver and kidney of mice. Ach. Toxicol., 85: 327-35
Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 246:7130-7139
Hafeman, D. G., Sunde, R. A. and Hoekstra, W. G. (1984). Effect of dietary selenium erythrocyte and liver glutathione peroxidise in the rat. J. Nutr., 104:580-587
Hashmi, I. and Khan, A. D. (2011). Adverse health effects of pesticides exposure in agricultural and industrial workers of developing country. Pesticides—The Impacts of Pesticides Exposure. http://dx. doi. org/10.5772/
Hammam, M. A. and Abdel-Mottaleb, E. M. (2007). Studies of the genotoxic and histopathological effects of the organophosphorus insecticide "profenofos" on white rats. Egypt J. Hosp. Med., 29: 685-706
Hassar, G. M. and Mazzan, K. H. M. (2011). Genotoxicity and histopathological studies on the liver, kidney and lymphocytes of male rats fed on diet containing waste fat released from chicken during grilling process. J. Cytol. Histol., 2:111
Heikal, T., Mossa, A. T., Rasoul, M. and Marei, H. (2013). The ameliorating effects of green tea extract against cyromazine and chlorpyrifos induced liver toxicity in male rats. Asian J. Pharma. Clin. Res., 6(1):48-55
Khan, S. and Kumari, D. O. O. J. (2011). Analytical study of histopathological changes induced by of Endosulfan in kidney of albino rats. Asian J. Pharma.Clin. Res., 5(1): 113-114
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, A. J. (1951). Protein measurement with folin phenol
reagent. J. Biol. Chem., 193:265-275
Marklund, S. and Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Euro. J. Biochem., 47:469-474
Mansour, S.A and Mossa, A.H. (2010). Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc. Pesticide. Biochem. Physiol., 96: 14-23. DOI: 10.1016/j.pestbp.2009.08.008.
Maroni, M., Colosio, C., Ferioli, A. and Fait, A. (2000). Toxicology: review. Toxicology, 143; 5-91
Mittal, S., Kaur, G. and Vishwakarma, G. S. (2014). Effects of Environmental Pesticides on the Health of Rural Community in the Malwa Region of Punjab, India: A Review”. Human Ecol. Risk Assessment Int. J., 20 (2): 366-387
Owoeye, O., Edem, F., Akinyoola, B., Rahaman, S., Akang, E. and Arinola, G. (2012). Histological changes in liver and lungs of rats exposed to dichlorvos before and after vitamin supplementation. Eur. J. Anat., 16 (3): 190-198
Sharma, D. and Sangha, G. K. (2014). Triazophos induced oxidative stress and histomorphological changes in liver and kidney of female albino rats. Pesticide Biochem. Physiol., 110: 71-80
Shetty, P. K. (2004). Socioecological implications of pesticide use in India. Economic and Political Weekly, 5261–5267
Stocks, J. and Dormandy, T. L. (1971). The autoxidation of human red cell lipids induced by hydrogen peroxide. British J. Haem., 20(1):95-111
Thakur, J. S., Rao, T. B., Rajwanshi, A., Parwana, H. K. and Kumar, R. (2008). Epidemilogical study of high cancer among rural agricultural community of Punjab in northern India. Int. J. Environ. Res. Pub. Health, 5:399-4
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Kalra, S., & Sangha, G. (2017). Pesticides induced oxidative stress and histomorphological changes in liver and kidney of female Bandicota bengalensis and Tateraindica. Journal of Applied and Natural Science, 9(2), 935-939. https://doi.org/10.31018/jans.v9i2.1300
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