Gurmeet Kaur Veena Khanna


PGPR strains exhibiting optimum functional traits at high temperature and are compatible with Rhizobium can be used in pigeonpea as biofertilizer. A total of 45 rhizobacterial isolates were isolated from 13 different locations of pigeonpearhizospheric soil of Punjab. Out of the 45 isolates, 5 isolates selected on the basis of maximum growth at 30°C and 40°C were morphologically and biochemically characterized, belonging to genera Pseudomonas (P-6, P-9) and Bacillus (P-30, P-31, P-32). Selected isolates were further evaluated for the production of IAA, GA, SA and flavonoids. IAA production was estimated in the range from 0.45-25.13 ?g/ml and 4.62-34.34 ?g/ml in the presence of tryptophan at 30 and 40°C respectively. Maximum gibberellic acid production was recorded with P-30 (108.99 ?g/ml and 112.12 ?g/ml) at 30 and 40°C respectively. Similarly maximum salicylic acid was also estimated with P-30 (157.2 ?g/ml) followed by P-31 (141.0 ?g/ml) at 40°C. All the isolates were also found to produce flavonoids ranged from 2.98 - 4.40 ?g/ml at 40 °C. Isolates P-30, P-31 showed superior production of growth hormones and flavonoid-like compounds can further be tested under the field conditions to enhance growth and yield of pigeonpea.




Pigeonpea, PGPR, Rhizobium, Rhizobacteria

Anonymous (2016). Package and practice for kharif crops. Pp. 62. Punjab Agricultural University, Ludhiana.
Ali, S.Z., Sadhya, V., Grover, M., Kishore, N., Rao, L. V. and Venkateswarlu, B. (2009). Pseudomonas sp. strain AKM-P6 enhances tolerance of sorghum seedlings to elevated temperatures. Biol. Fertil. Soils., 46: 45-55
Borrow, A., Brain, P. W., Chester, V. E., Curtis, P. J., Hemming, H. G., Henehan, C., Jeffereys, E. G., Lloyd, P. B., Nixon, I. S., Norris, G. L. F. and Radley, M. (1955). Gibberellic acids a metabolic product of the fungus Gibberellafujikuroi some observations on its production and isolation. J Sci Food Agric., 6: 340-48
Bhattacharyya, P. N. and Jha, D. K. (2012). Plant growth promoting rhizobacteria: emergence in agriculture. World J. Microbiol. Biotechnol., 28: 1327-1350
Cappuccino, J. C. and Sherman, N. (1992). Microbiology: A Laboratory Manual Pp.125-79. Academic distributors, New York.
Chakraborty, U. and Tongden, C. (2005). Evaluation of heat accumulation and salicylic acid treatments as potent inducers of thermotolerance in Cicerarietinum L. Curr. Sci., 89: 384-89
Choudhary, A. K., Kumar, S., Patil, B. S., Bhat, J. S., Sharma, M., Kemal, S., Ontagodi, T. P., Datta, S., Patil, P., Chaturvedi, S. K., Sultana, R., Hedge, V. S., Choudhary, S., Kamannavar, P. Y. and Vijayakumar, A. G.(2013). Narrowing yield gaps through genetic improvement for Fusarium wild resistance in three pulse crops of the semi-arid tropics. Subbarao J Breed Genet., 45(3): 341-70
Gordon, A. S. and Weber, R. P. (1951). Calorimetric estimation of Indole acetic acid. Pl Physiol., 25: 192-95
Holt, P. S., Burh, R. J., Cunnigham, D.L. and Porter, R. E. (1994). Effect of two different molting procedures on Salmonellaenteritis infection. Poult Sci., 73: 1267-75
Kaur, S. and Khanna, V. (2013). Effect of temperature-tolerant rhizobial isolates as PGPR on nodulation, growth and yield of Pigeonpea. J Food Legumes, 26: 80-83
King, E. O., Ward, M. K. and Raney, D. E. (1954). Two simple media for the demonstration of pyocyanin and fluorecein. J Lab Clin Med., 44:301-07
Mallikarjuna, N., Saxena, K. B. and Jadhav, D. R. (2011). Cajanus. In: Kole, C. (ED.), Wild crop relatives: Genomic and breeding resources, legume crops and forages. Springer-Verlag, Berlin, Heidelberg Pp. 21-33
Meyer, J. M. and Abdallah, M. A. (1978). The fluorescent pigment of Pseudomonas fluorescence: biosynthesis, purification and physicochemical properties. J Gen Microbiol., 107:319-28
Mia, M. A. B., Shamsuddin, Z. H. and Mahmood, M. (2012). Effects of rhizobia and plant growth promoting rhizobacteria inoculation on germination and seedling vigor of lowland rice. Afr J Biotechnol., 11: 3758-65
Nadeem, S. M., Ahmad, M., Zahir, A. Z., Javaid, A. and Ashraf, M. (2014). The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnol adv,. 32: 429-48
Nehra, K., Yadav, A.S., Sehrawat, A.R. and Vashisha, R.K. (2007). Characterization of heat resistant mutant strains of Rhizobium sp. (CajanuscajanL.) for growth, survival and symbiotic properties. Ind J Microbiol4: 329-35.
Odney., (2007). The potential of pigeonpea (Cajanuscajan(L.) Millsp.) in Africa. Natural Resources Forum. 31: 297-05.
Parmar, N. and Dadarwal, K.R. (1999). Stimulation of nitrogen fixation and induction of flavonoid like compounds by rhizobacteria . J ApplMicrobiol.86: 36-44.
Ruchi, K.R., Kumar, A., Patil, S., Thapa, S. and Kaur, M. (2012). Evaluation of plant growth promoting attributes and lytic enzyme production by fluorescent Pseudomonas diversity associated with apple and pear. Int J Sci Res Pub 2: 2250-3153.
Srivastava, S., Yadav, A., Mishra, S., Chaudary, V. and Srivastava, C.S. (2008) Effect of high temperature on Pseudononasputida NBRI0987 biofilm formation and expression of stress sigma factors RpoS. Curr Microbial 56: 453-457.
Trivedi, P., Anita, P., Lok, M. and Palni, S. (2008). In vitro evaluation of antagonistic properties of Pseudomonas corrugata. Microbiol Res 163: 329-36.
Zhang, S., Moyne, A., Reddy, M.S. and Kloepper J.W. (2002). The role of salicylic acid in induced systemic resistance elicited by plant growth-promoting rhizobacteria against blue mold of tobacco. Biol Control 25 (3):288-96.
Zhishen, J., Mengcheng, T. and Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555–59.
Research Articles

How to Cite

Evaluation of thermotolerant rhizobacteria for multiple plant growth promoting traits from pigeonpea rhizosphere. (2017). Journal of Applied and Natural Science, 9(2), 920-923. https://doi.org/10.31018/jans.v9i2.1298