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Shrvan Kumar Asha Sinha Meena Shekhar Vimla Singh

Abstract

Aflatoxin B1 (AFB1) is most frequently found in plant substrates, which has shown the highest toxigenic potential. Based on previous studies, the IARC has classified AFB1 as a class 1A human carcinogen. Several impacts on consumers, such as loss of human and animal lives; health care and veterinary care costs; contaminated foods and feeds disposal costs; and investment in research and management of the myco-toxin problem. Fourteen maize seed samples comprising of recommended and local varieties were collected from three maize growing zones (Zone I- Almora, Kullu, Bilaspur, Dhaulakuan, Kangara, Saharanpur, Zone II- Karnal, Ludhiana, Pantnagar, New Delhi and Zone III- Begusarai, Varanasi, Sabour-1 and Sabour 2). In our studies AFB1 toxin range were noticed Zone-I (0.0294- 153.5081 ppb), Zone-II (0.1761- 161.0537 ppb ppb) and Zone-III (3.8366- 53.1256 ppb) collected seed samples.This indicate that ELISA technique could be applied to the monitoring of Aflatoxin contamination in a lot of samples in a cost, accuracy, simplicity and time effective manner.

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Keywords

AFB1 toxin, Aspergillusflavus, Indirect-ELISA, Maize

References
Abbas, A. K., Zblotowicz, R. M., Weaver, M. A., Horn, B. W., Xie, W. and Shier, W. T. (2004). Comparision of cultural and analytical methods for determination of Aflatoxin produced by Mississippi Delta Aspergillus isolates. Can. J. Microbio., 50: 193–199
Abbas, H. K., Cartwright, R. D., Xie, W. and Shier, W. T. (2006). Aflatoxin and fumonisin contamination of corn (maize, Zea mays) hybrids in Arkansas. Crop Prot.,25:1-9
Alptekin Yasar, Duman, Ahmet Dogan, Akkaya and Murat Reis (2009). Identification of fungal genus and detection of Aflatoxin level in second crop corn grain. Journal of Animal and Veterinary Advances, 8(9):1777–1779
Ayejuyo, O. O., Olowu, R. A., Agbaje, T. O., Atamenwan, M. and Osundiya, M. O. (2011). Enzyme - Linked immunosorbent assay (ELISA) of Aflatoxin B1 in groundnut and Cereal Grains in Lagos, Nigeria. Research Journal of Chemical Sciences, 1(8): 1–5
Chun, H. S., Kim, H. J., Ok, H. E., Hwang, J. and Chung, D. (2007). Determination of Aflatoxin levels in nuts and their products consumed in South Korea, Food Chemistry, 102: 385–391
Kumar, S., Sinha, A., Hooda, K. S. and Singh, V. (2015). Advanced detection techniques of toxigenic fungi and their management strategies. In: Sixth IJAA-JSPS, International conference on Contemporary Advances of Science and Technology, 7-9 Aug., p.145
Moss, M. O. (1998). Recent studies of mycotoxins. J. Appl. Microbiol. Symposium Suppl. 84: 62S–76S
Ono, E.Y.S., Ono, M.A., Funo, F.Y., Medina, A.E., Oliveira, T.C.R.M., Kawamura, O., Ueno, Y. and Hirooka, E.Y. (2001). Evaluation of fumonisin Aflatoxin co-occurrence in Brazilian corn hybrids by ELISA. Food Additives and Contaminants, 18(8):719–729
Oplatowska-Stachowiak, M., Sajic, N., Xu Y., Haughey, S. A., Mooney, M. H., Gong Y. Y., Verheijen, R. and Elliott, C.T. (2016). Fast and sensitive Aflatoxin B1 and total Aflatoxins ELISAs for analysis of peanuts, maize and feed ingredients. Food Control, 63:239–245
Rothschild, L. J. (1992). IARC classes AFB1 as class 1 human carcinogen. Food Chem., 34: 62–66
Saleemi, Muhammad Kashif, Khan, Muhammad Zargham Khan, Ahrar (2012). Occurrence of toxigenic fungi in maize and maize-gluten meal from Pakistan. Phytopathologia Mediterranea, 51(1): 219–224
Section
Research Articles

How to Cite

Detection of Aflatoxin B1 through indirect ELISA from fresh grains obtained from three maize growing zones of India. (2017). Journal of Applied and Natural Science, 9(2), 796-798. https://doi.org/10.31018/jans.v9i2.1278