Analysis of seed protein diversity in Cicer arietinum L. genotypes with different seed coat colour using SDS-PAGE
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Abstract
SDS-PAGE technique was used for the study of seed protein polymorphism among three genotypes of Cicer arietinum with different seed coat colour. A total of 24 polypeptide bands were recorded. Out of these 20 were common among all three genotypes and 4 (16.66%) were polymorphic. The data analysis using UPGMA clustering revealed that genotypes with C2 (dark brown) and C3 (black) were closer as compared to genotype with C1 (light brown) coat colour. Jaccard similarity coefficient value ranged from 0.87 to 0.92. The similarity matrix was subjected to UPGMA clustering to generate dendrogram. The most closely revealed genotypes were C2 (dark brown) and C3 (black) with the highest similarity index 0.92 whereas, C1 (light brown) showed minimum similarity index with C3 (black) genotype 0.87. Each of three genotypes of C.arietinum had some polypeptide bands which were peculiar to them only. This enabled distinguishing all three genotypes on the basis of specific polypeptide fragments using SDS-PAGE analysis.
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References
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Diversity In Abrus Precatorius Genotypes With
Different Seed Coat Colour. Int. J. Life Sci.Biotech. Pharma Res.,1 (2): 268-277
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Singh, P.K., Shrivastava, N., Chaturvedi, K., Sharma, B. and Bhagyawant, S. (2015). Characterization of seed storage proteins from chickpea using 2D electrophoress coupled with mass spectrometry. Biochem res int., 1: 1-6
Smith, J.S.C. and Smith, O.S. (1986). Environmental effects on Zein Chromatograms of maize inbred lines revealed by reversed-phase high performance liquid chromatography. Theor.Appl. Genet., 71: 607-612
Smýkal, P., Coyne, C.J., Ambrose, M.J., Maxted, N., Schaefer, H., Blair, M. W., Berger,J., Greene, S. L., Nelson, M., Besharat, N., Vymyslicky, T., Toker, C., Saxena, R.K., Roorkiwal, M., Pandey, M. K., Hu, J.,. Li, Y.H., Wang, Guo, Y., Qiu, Li., Redden, R.J., and Varshney, R. K. (2015). Legume crops phylogeny and genetic diversity for science and breeding. Critical Reviews in Plant Sciences, 34 (1-3), 43-104
Toker, C., Uzun, B., Ceylan, F. O. and Ikten, C. (2014). Chickpea. In: Alien Gene Transfer in Crop Plants, A. Pratap and J. Kumar Eds., Volume 2, Springer, Dordrecht, Pp. 121-151
Ahmad, F. and Slinkard, A.E. (1992). Genetic relationships in the genus CicerL. as revealed by polyacrylamide gel electrophoresis of seed storage proteins.Theor. Appl. Genet., 84: 688-692
Akbar, A., Ahmed, H., Ghafoor, S. and Khan, A.I. (2010). Phylogeny and genetic diversity studies in Capsicum using seed storage proteins.Curr. Res. J. Biol. Sci., 2 (4): 250-252
Aliu, S., Kaul, H.P., Rusinovci, I, Mayrhofer, V.S., Fetahu, S. and Zeka, D. (2016). Genetic diversity for some
nutritive traits of chickpea (Cicer arietinum L.) from different regions in Kosova. Turkish J. Field crops, 21 (1) 156-161
Arefian, M., Vessal, S. and Bagheri, A. (2014). Biochemical changes and SDS-PAGE analyses of chickpea (Cicer arietinum L.) genotypes in response to salinity during the early stages of seedling growth. J. Biol. Environ. Sci., 8 (23): 99-109
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantity of protein utilizing the principle ofprotein-dye binding. Anal Biochem., 72: 248-254
Chittora, M. and Purohit, S.D.(2012). Optimization of SDS-PAGE Conditions and Analysis Of Seed Protein
Diversity In Abrus Precatorius Genotypes With
Different Seed Coat Colour. Int. J. Life Sci.Biotech. Pharma Res.,1 (2): 268-277
Cook, R.J. (1984). The characterization and identification of crop cultivars by electrophoresis. Electrophoresis., 5: 65-72
Fergusan, J.M. and Grabe, D.F. (1986). Identification of cultivars of perennial rye grass by SDS-PAGE of seed proteins.CropSci., 26:170-176
Food and Agricultural Organization of the United Nations. (2013). FAO Statistical Databases. Available at http://faostat.fao.orgl FAO, Rome.
Gepts, P. (1990). Genetic diversity of seed storage proteins in plants.Plant PopulGenet, Breeding and Genet
Resour., 64-82
Gupta, D., Chellapila, B. and Ranjan, R. (2016). Genetic diversity analysis of chickpea (Cicer arietinum L.)
using seed protein profiling. Int. J. Innovative Res. multidisciplinary field, 2 (9): 1-10
Hameed, A., Shah, T.M., Atta, B.M., Iqbal, N., Haq, M.A. and Ali, H. (2009). Comparative seed storage protein profiling of kabuli chickpea genotypes. Pak. J. Bot., 41 (2): 703-710
Inamullah., Khan, I.A., Ahmed, H., Ghafoor, S.,Gul, S., Muhammad, I. and Ilyas, M. (2010). Seed storage protein profile of rice varietiescommonly grown in Pakisthan. Asian J of Agricultural sciences, 2 (4): 120-123
Khan, M.W., Khan, I.A., Ahmed, H., Ali. H., Gafoor, S., Afzal, M., Khan, F.A., Shah, M. and Afridi, S.G. (2010) Estimation of genetic diversity in walnut. Pak. J. Bot., 42 (3): 1791-1796
McClean, P.E., Lee, R.K., Otto, C., Gepts, P. and Bessett, M.J. (2002). Molecular and phenotypic mapping of genes controlling seed coat pattern and colour in common bean (Phaseolus vulgaris L.).The J.Heridity., 93: 148-152
Mukherjee, M. and Datta, A.K. (2008), Evaluation of genetic diversity in five species of Ocimumby SDS-PAGE.IndianJ. Genet. Plant Breed, 68: 212-214
Mukhlesur, R.M., Hirata, Y. and Alam, S.E. (2004). Genetic variation within Brassicarapacultivars using SDS-PAGE for seed protein and isozyme analysis.J. Biol. Sci., 4(2): 234-238
Raymond, J., Inquello, V. and Azanza,J.L. (1991). The seed protein of sunflower: comparative studies of cultivars. Phytochemistry, 30: 2849-2856
Siddiqui, M.F. and Naz, N. (2009). Protein landmarks for diversity assessment in wheat genotypes”.African J Biotech., 8 (9): 1855-1859
Singh, P.K., Shrivastava, N., Chaturvedi, K., Sharma, B. and Bhagyawant, S. (2015). Characterization of seed storage proteins from chickpea using 2D electrophoress coupled with mass spectrometry. Biochem res int., 1: 1-6
Smith, J.S.C. and Smith, O.S. (1986). Environmental effects on Zein Chromatograms of maize inbred lines revealed by reversed-phase high performance liquid chromatography. Theor.Appl. Genet., 71: 607-612
Smýkal, P., Coyne, C.J., Ambrose, M.J., Maxted, N., Schaefer, H., Blair, M. W., Berger,J., Greene, S. L., Nelson, M., Besharat, N., Vymyslicky, T., Toker, C., Saxena, R.K., Roorkiwal, M., Pandey, M. K., Hu, J.,. Li, Y.H., Wang, Guo, Y., Qiu, Li., Redden, R.J., and Varshney, R. K. (2015). Legume crops phylogeny and genetic diversity for science and breeding. Critical Reviews in Plant Sciences, 34 (1-3), 43-104
Toker, C., Uzun, B., Ceylan, F. O. and Ikten, C. (2014). Chickpea. In: Alien Gene Transfer in Crop Plants, A. Pratap and J. Kumar Eds., Volume 2, Springer, Dordrecht, Pp. 121-151
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Analysis of seed protein diversity in Cicer arietinum L. genotypes with different seed coat colour using SDS-PAGE. (2017). Journal of Applied and Natural Science, 9(2), 706-709. https://doi.org/10.31018/jans.v9i2.1261
