Buckeye rot disease of tomato which is one of the most devastating diseases of tomato crop is caused by soil born fungus Phytophthora nicotianae var. parasitica. In present study, the pathogen was isolated, morphologically identified and its pathogenicity was proved on susceptible commercial variety Solan Lalima and resistant line EC-251649 of tomato. Isolation of pathogen from the infected tomato fruit was achieved on Corn Meal Agar (CMA) out of two different media viz., Potato Dextrose Agar (PDA) and CMA. Fungal inoculum was prepared on Corn Meal broth. Inoculation with 10 ml of inoculum was found optimum in plant parts namely, stem, leaves and fruits. Parental lines were inoculated to test their disease reaction to buckeye rot. Symptoms of infection appeared on leaves and fruits only. Solan Lalima was found to be highly susceptible to with disease severity of 92 % and 100 % disease incidence, while EC-251649 was found moderately resistant on the basis of 16 % disease severity and 10 % disease incidence to the disease. After confirmation of resistance and susceptibility, the parental lines were surveyed for polymorphism using 42 primers and 32 were recorded to be polymorphic revealing that the differences are present at DNA level also. This is the very first study which evaluated parental lines for buckeye rot disease reaction on morphological as well as molecular basis. These lines will be further used for quantitative trait loci (QTL) analysis/gene tagging for buckeye rot and marker assisted selection to provide improved varieties to the farmers.
Medium, Molecular markers, Polymorphism, Tomato
Lu, M., Han, Z. and Yao, L. (2013). In vitro and in vivo antimicrobial efficacy of essential oils and individual compounds against Phytophthora parasitica var. nicotianae. J. Appl. Microbiol., 115 (1) : 187-198
Bijaya, A.K., Tewari, R.N. and Verma, T.S. (2002). Screening of tomato varieties/breeding lines against buckeye rot Phytophthora nicotianae var. parasitica Dastur. Indian. J. Hill., Fmg. 15(1):39-43
Bowers, J. H. and Locke, J. C. (2004). Effect of formulated plant extracts and oils on population density of Phytophthora nicotianae in soil and control of Phytophthora blight in the greenhouse. Plant. Dis., 88 (1):11-16
Dodan, D. S., Shyam, K. P. and Bhardwaj, S.S. (1995). Relative response of tomato cultivar lines against Buckeye rot under field conditions. Pl. Dis. Res., 10(2):135-136
Doyle, J. J. and Doyle, J. J. (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem. Bul., 19 : 11-15
FAOSTAT. (2015). Global tomato production in 2013; Crops/World/2013. UN Food and Agriculture Organization, Statistics Division. 2015. Retrieved 3 June 2016.
Flores, V. D. I., Gonzalez, G. M. and Ramirez, O. R. (2013). Proposal of a culture medium for the study of Phytophthora nicotianae Breda de Haan. Revista de Cienciay Tecnologia, (19) : 24-27
Gupta, S. K., Upmanyu, S. and Sharma, R. C. (2005). Biology, epidemiology and management of buckeye rot of tomato. In: challenging problems in horticultural and forest pathology. Sharma, R. C. and Sharma, J. N. (eds.). Indus Publishing Co., New Delhi, India Pp. 183-199
Jain, S. S., Sharma, S. L. and Juneja, S. L. (1961). Studies on buckeye rot of tomato-new toHimachal Pradesh. Proceedings of Indian Science Congress Association, 50 : 351-352
Kaur, R., Shilpa, Vaidya, E. and Kumar, K. (2015) Development, characterization and transferability of peach genic SSRs to some Rosaceaespecies. Advances in Research, 3 :165-180
Mehta, S. (2004). Studies on resistance to some fungal diseases and heterosis in tomato (Lycopersicon esculentum Mill.). Ph.D. Thesis. Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, HP, India.
Olanya, O. M. and Larkin, R. P. (2006). Efficacy of essential oils and biopesticides on Phytophthora infestans suppression in laboratory and growth chamber studies. Biocontrol. Sci. Technol., 16 (9/10) : 901-917
Oliva-Risco, E. (1983). Methods of assessing resistance to Phytophthora infestans in tomato. Centro Agricola., 10(2) : 35-46
Ribeiro, O. K. (1978). A source book of the genus Phytophthora. Cramer, Vaduz, Liechtenstein. 417p
Sherbakoff, C. D. (1917). Buckeye rot of tomato fruit. Phytopathology, 7 : 119-129
Tiwari, R. K., Mistry, N. C, Singh, B. and Gandhi. C. P. (2014). (eds.). Indian Horticulture Database. Gurgaon: National Horticulture Board Pp. 177-185
Wani, A. H. (2011). An overview of the fungal rot of tomato. Mycopathology, 9(1):33-38
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)