Scanning electron microscopic studies of Beauveria bassiana against Lipaphis erysimi Kalt
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Abstract
This work was aimed to identify the LC50 of the indigenous fungal isolates for controlling L. erysimi infesta-tion in mustard aphid besides to probe the mechanism of action of the local isolates and comparison of the efficacy with the reference culture and commercial formulation ‘Mycojaal’. Three isolates of entomopathogenic fungi Beauveria bassiana were tested for infection on nymph of Lipaphis erysimi Kalt. using scanning electron microscopy (SEM) to record any variation. The SEM revealed adhesion of spores of B.bassiana followed by penetration of L.erysimi nymph surface. It was observed that all Beauveria isolates showed little variation with respect to penetration and adhesion at different time intervals. Further, lethal concentration (LC50) values of B. bassiana isolates against L. erysimi was recorded and was lowest (0.05x107 spores/ml) in B. bassiana MTCC 4495 and highest (0.11.X107 spores/ml) was recorded in native isolate F10 after 120 hours of treatment. The study has established the need for the isolation and evaluation of the indigenous Beauveria isolate. Moreover, it also exhibited the efficacy of the reference and commercially available biocontrol agents.
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Beauveria bassiana, Lipaphis erysimi, Lethal concentration, Scanning electron microscopy
Alves, S.B. (1998). Fungosentomopatogenicos. Alves S B (ed) Controle Microbaino de Insectos. Pp. 289-382. FEALQ, Piracicaba, Brazil.
Cooke, R. C. (1977). Fungi, man and his environment, Longman, London.
Devi, P.S.V., Prasad, Y.G., Chowdary, Y.G.A., Rao, D.M. and Balakrishnan, L.K. (2003). Identification of virulent isolates of the entomopathogenic fungus Nomuraearileyi (F) Samson for the management of Helicoverpaarmigera and Spodopteralitura. Mycopathol., 156: 365-73
Gabarty, A., Salem, H.M., Fouda, M.A., Abas, A.A. and Ibrahim, A.A. (2014). Pathogencity induced by the entomopathogenic fungi Beauveriabassiana and Metarhiziumanisopliae in Agrotisipsilon. J radiation res. and appl sci., 95-100
Gurr, G.M., Wratten, S.D. and Altieri, M. (2004). Ecological Engineering for Pest Management. Advances in Habitat Manipulation for Pest Management. CSIRO Publishing, Melbourne, Australia.
Harrington, R. and Van Emden, H.F. (2007). Aphids as Crop Pests. CABI Publishing, UK.
Jiji, T., Praveena, R., Babu, K., Naseema, A. and Anitha, N. (2006). Three promising fungal strains pathogenic to fruit flies of economic importance from basic to applied knowledge. Proceedings of the 7th International symposium on fruit flies of economic importance. Salvador, Brazil. 175-77
Li, J., Guo, Q., Lin, M., Jiang, L., Ye, J., Chen, D., Li, Z., Dai, J. and Han, S. (2016). Evaluation of a new entomopathogenic strain of Beauveriabassiana and a new field delivery method against Solenopsisinvicta. PLoS ONE 11(6): e0158325. doi:10.1371/journal. pone. 0158325
Luz, C., Tigano, M.S., Silva, I.G., Cordeiro, C.M.T. and Alijanabi, S.M. (1998). Selection of Beauveria bassiana and Metarhiziumanisopliae isolates to control Triatomainfestans. Mem.Inst. Oswaldo Cruz., 93:839-846
Moguel, J.C., Mier, T., Montes, M.D.R., Barranco, H.N., Rodriguez, A.Z., Torres, A.P. and Toriello, C. (2013). Fungal growth development index and ultrastructural study of whiteflies infected by three Isariafumosorosea isolates of different pathogenicity. Universidad Nacional Autonoma de Mexico.
Moino, Jr, A., Alves, S.A., Lopes, R.B., Neves, P.M.O.J., Pereira, R.M. and Vieira, S.A. (2002). External development of the entomopathogenic fungi Beauveriabassiana and Metarhiziumanisopliae in the subterranean termite Heterotermes tenuis. Sci. Agric., 59: 267-273
Ormond, E.L., Thomas, A.P.M., Pugh, P.J.A., Pell, J.K. and Roy, H.E. (2010). A fungal pathogen in time and space. The population dynamics of Beauveria bassiana in a conifer forest. FEMS Microbiol. Ecol., 74: 146-54
Quesada-Moraga, E., Landa, B. B., Muñozledesma, J., Jiménez-Díaz, R. M. and Santiago Álvarez, C. (2006). Endophytic colonization of opium poppy Papaversomniferum by an entomopathogenic Beauveria bassiana strain. Mycopathol., 161:323-29
Sahoo, S.K. (2012). Incidenc Alizadeh, A., Kharrazi Pakdel, A., Talebi-Jahromi, K.H. and Samih, M.A., ( 2007). Effect of some Beauveria bassiana (Bals). Vull. isolates on common Pistachio Psylla Agonoscena pistaciae Burck. Int. J. Agri. Biol., 9:76-79
Sun, X., Yan, W., Zhang, J., Niu, X., Li, F., Qin, W. and Ma, G. (2016). Frozen section and electron microscopy studies of the infection of the red palm weevil, Rhynchophorusferrugineus (coleoptera:curculionidae) by the entomopathogenic fungus Metarhizium anisopliae. SpringerPlus, 5:1748. DOI 10.1186/s40064-016-3416-6
Talaei-hassanloui, R., Kharazi-pakdel, A., Goettel, M.S., Little, S. and MozaVari, J. (2007). Germination polarity of Beauveria bassiana conidia and its possible correlation with virulence. J. Invert. Pathol., 94:102–107
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