Identification and characterization of Pseudomonas fluorescensstrains effective against Xanthomonasoryzaepv. oryzae causing bacterial blight of rice in Punjab, India
Article Main
Abstract
For the control of bacterial blight of rice caused by Xanthomonasoryzaepv. oryzae, sixty four Pseudomonas fluorescens strains were recovered from rice and wheat rhizosphere. These strains were identified on the basis of internal transcribed spacer (ITS) region. It was observed that the strains showing fluorescence in the selective media showed the amplification of the targeted P. fluorescens specific ITS region. The strains were also characterized for the production of the antibiotic 2, 4-diacetylphloroglucinol (DAPG) using phlDlocus. The characteristic 750bp region was amplified in all the DAPG producing strains. These strains were evaluated against X. oryzae in vitro by dual culture method. The P. fluorescens strains found effective in vitro were further tested in field for their antagonistic potentiality and disease suppression ability. P. fluorescens strain number Pf-4-R showed maximum inhibition i.e. of 5.5 mm against the test pathogen X. oryzaepv. oryzae. Talc based powder formulation of the effective strain Pf-4-R used for field evaluation, showed that pre-inoculation foliar sprays were effective in controlling bacterial blight of rice with disease suppression efficiency ranging from 29.6 to 65.6 percent in different treatments.
Article Details
Article Details
Keywords
Bacterial blight, phl, Pseudomonas fluorescens;, Xanthomonasoryzaepv. oryzae
References
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Dowling, D.N. and O’Gara, F. (1994). Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends in Biotechnology 12: 133–141
Ghasemiet, E., Kazempour, M.K. and Padah, F. (2008). Isolation and identification of Xanthomonas oryzae pv. oryzae the causal agent of bacterial blight of rice in Japan. Journal of Plant Protection 48: 53-62
Gnanamanickam, S.S. and Mew, T.W. (1992). Biological control of blast disease of rice (Oryza sativa L.) with antagonistic bacteria and its mediation with a Pseudomonas antibiotic. Annals of Phytopathological Society of Japan 58: 380–385
Jambhulkar, P.P. and Sharma, P. (2014). Development of bioformulation and delivery system of Pseudomonas fluorescens against bacterial leaf blight of rice (Xanthomonas oryzae pv. oryzae). J. Environ Biol. 35:843-849
Jayalakshmi, C., Madhiazhagan K. and Rettinassababady C. (2010). Effect of different methods of application of Pseudomonas fluorescens against bacterial leaf blight under direct sown rice. J. Biopest. 3:487– 488
Jeung, J.U., Heu, S.G., Shin, M.S., Cruz, C.M. and Jena, K.K. (2006). Dynamics of Xanthomonas oryzae pv oryzae population in Korea and their relationship to known bacterial blight resistance genes. Phytopathology 96: 865-75
Kar, A., Mandal, K. and Singh, B. (2013). Environmental fate of chlorantraniliprole residues on cauliflower using QuEChERS technique. Environmental Monitoring and Assessment 185: 1255-1263
Kaufmann, H.E., Reddy, A.P.K., Hseih, S.P.Y. and Merca, S.S. (1973). An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Disease Reporter 57: 537-541
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Landa, B.B., Mavrodi, D.M., Thomashow, L.S. and Weller, D.M. (2003). Interaction between strains of 2,4-Diacetylphloroglucinol producing Pseudomonas fluorescens in the rhizosphere of wheat. Phytopathology 93: 982-994
Lee, K.S., Rasabandith, S., Angeles, E.R. and Khush, G.S. (2003). Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology 93: 147-52
Lore, J.S., Vikal, Y., Hunjan, M.S., Goel, R.K., Bharaj, T.S. and Raina, G.L. (2011). Genotypic and pathotypic diversity of Xanthomonas oryzae pv. oryzae, the cause of bacterial blight of rice in Punjab state of India. Journal of Phytopathology 159: 479-87
Manav, M. and Thind, B.S. (2002). Management of bacterial blight of rice with bioagents. Plant Disease Research 17: 21-28
Mew, T.W. and Rosales, A.M. (1986). Bacterization of rice plants for control of sheath blight caused by Rhizoctonia solani. Phytopathology 76: 1260–1264
Niranjana, S.R., Lalitha, S. and Hariprasad P. (2009). Mass multiplication and formulations of biocontrol agents for use against fusarium wilt of pigeonpea through seed treatment. Int. J. Pest Mgt. 55: 317-324
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Papavizas, G.C. and Lewis, J.A. (1983). Physiological biocontrol characteristics of stable mutants of
Trichoderma viride resistance to MBC fungicides. Phytopathology, 73: 407–411
Raaijmakers, J.M., Bonsall, R.F. and Weller, D.M. (1999). Effect of population density of Pseudomonas
fluorescens on production of 2,4-diacetylphloroglucinol in the rhizosphere of wheat. Phytopathology 89: 470–475
Raaijmakers, J.M., Weller, D.M. and Thomashow, L.S. (1997). Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Applied and Environmental Microbiology 63: 881–887
Ramanamma, C., Bharathi, G. and Sreeramula, A. (1994). Paddy rhizosphere bacterium inhibiting rice plant pathogens. Journal of Ecotoxicology and Environmental Monitoring 4: 65-69
Rangarajan, S., Saleenal, L.M., Vasudevan, P. and Nair, S. (2003). Biological suppression of rice diseases by
Pseudomonas spp. under saline soil conditions. Plant and Soil 251: 73-82
Rishnamurthy, S. and Gnanamanickam, S.S. (1998) Biocontrol of rice sheath blight with formulated Pseudomonas putida. Indian Phytopathology 51: 233-36
Rodriguez, F. and Pfender, W.F. (1997). Antibiosis and antagonism of Sclerotinia homo eocarpa and Drechslera poae by Pseudomonas fluorescens PF-5 in vitro and in planta. Phytopathology 87: 614–621
Sakthivel, N., Swamani, E., Anuratha, C.S., Savithiry, S. and Gnanamanickam, S.S. (1988). Advances in research on plant pathogenic bacteria. In S.S. Gnanamanickam and A.J. Mahadevar (Eds.) Proceedings of National Symposium on Phytobacteriology held at the University of Madras, Madras, India, March 14-15, 1986 (pp. 213-220) New Delhi: Today and Tomorrow’s Printers and Publishers.
Santhi, D.P., Unnamalai, N. and Gnanamanickam, S.S. (1987). Epiphytic association of Erwinia herbicola with rice leaves infected by Xanthomonas oryzae pv. oryzae and its interaction with pathogen. Indian Phytopatholog 40: 327-32
Scarpellini, M., Franzetti, L. and Galli, A. (2004). Development of PCR assay to identify Pseudomonas fluorescens and its biotype. FEMS Microbiology Letters 236:257–260
Sharifi-Tehrani, A., Zala, M., Natsch, A., Moenne-Loccoz, Y. and Defago, G. (1998). Biocontrol of soil-borne fungal plant diseases by 2,4-diacetylphloroglucinol producing fluorescent pseudomonads with different restriction profiles of amplified 16S rDNA. European Journal of Plant Pathology 104: 631–643
Thakur, A., Singh, P.P. and Hunjan, M.S. (2011). Rhizosphere competence of Pf-4-R, a strain of Pseudomonas fluorescens colonizing rice roots. Plant Disease Research 26: 127-131
Thomashow, L.S., and Weller, D.M. (1995). Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites. In G. Stacey and N. Keen (Eds.) Plant-microbe interactions, Vol. 1 (pp. 187-235) New York: Chapman & Hall.
Velusamy, P., Defago, G., Thomashow, L. and Gnanamanickam, S.S. (2004). Role of 2,4-diacetylphloroglucinol for plant disease control: its importance to rice bacterial blight suppression in India. In L.V. Gangawane and C.D. Mayee (Eds.) Biotechnological approach for the integrated management of crop diseases (pp. 182-191) New Delhi: Daya Publishing House.
Velusamy, P., Immanuel, J.E., Gnanamanickam, S.S. and Thomashow, L. (2006). Biological control of rice
bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol. Can J Microbiol. 52:56-65.
Verma, K.K. (2009). Management of Meloidogyne javanica by bacterial antagonist Pseudomonas fluorescens as seedling root dip in tomato. Indian J. Nematol. 39: 207-210
Weller, D.M. (1983). Colonization of wheat roots by a fluorescent pseudomonad suppressive to take-all. Phytopathology 73:1548-1553
Anonymous, (2011). Package of Practices for Kharif crops, Punjab Agricultural University, Ludhiana, Pp 1-17.
Battu, P.R. and Reddy, M.S. (2009). Isolation of secondary metabolites from Pseudomonas fluorescens and its characterization. Asian Journal of Research in Chemistry 2: 26-29
Bharaj, T.S., Singh, N., Kaur, R., Bhullar, M.S. (2006). Genetic enhancement for yielding ability and bacterial blight resistance in rice (Abstract). Proc 2nd International Rice Congress, New Delhi, October 9–13, 2006, pp 296.
Dowling, D.N. and O’Gara, F. (1994). Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends in Biotechnology 12: 133–141
Ghasemiet, E., Kazempour, M.K. and Padah, F. (2008). Isolation and identification of Xanthomonas oryzae pv. oryzae the causal agent of bacterial blight of rice in Japan. Journal of Plant Protection 48: 53-62
Gnanamanickam, S.S. and Mew, T.W. (1992). Biological control of blast disease of rice (Oryza sativa L.) with antagonistic bacteria and its mediation with a Pseudomonas antibiotic. Annals of Phytopathological Society of Japan 58: 380–385
Jambhulkar, P.P. and Sharma, P. (2014). Development of bioformulation and delivery system of Pseudomonas fluorescens against bacterial leaf blight of rice (Xanthomonas oryzae pv. oryzae). J. Environ Biol. 35:843-849
Jayalakshmi, C., Madhiazhagan K. and Rettinassababady C. (2010). Effect of different methods of application of Pseudomonas fluorescens against bacterial leaf blight under direct sown rice. J. Biopest. 3:487– 488
Jeung, J.U., Heu, S.G., Shin, M.S., Cruz, C.M. and Jena, K.K. (2006). Dynamics of Xanthomonas oryzae pv oryzae population in Korea and their relationship to known bacterial blight resistance genes. Phytopathology 96: 865-75
Kar, A., Mandal, K. and Singh, B. (2013). Environmental fate of chlorantraniliprole residues on cauliflower using QuEChERS technique. Environmental Monitoring and Assessment 185: 1255-1263
Kaufmann, H.E., Reddy, A.P.K., Hseih, S.P.Y. and Merca, S.S. (1973). An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Disease Reporter 57: 537-541
Keel, C., Weller, D.M., Natsch, A., Defago, G., Cook, R.J. and Thomashow, L.S. (1996). Conservation of the 2,4- diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations. Applied and Environmental Microbiology 62: 552–563
Landa, B.B., Mavrodi, D.M., Thomashow, L.S. and Weller, D.M. (2003). Interaction between strains of 2,4-Diacetylphloroglucinol producing Pseudomonas fluorescens in the rhizosphere of wheat. Phytopathology 93: 982-994
Lee, K.S., Rasabandith, S., Angeles, E.R. and Khush, G.S. (2003). Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology 93: 147-52
Lore, J.S., Vikal, Y., Hunjan, M.S., Goel, R.K., Bharaj, T.S. and Raina, G.L. (2011). Genotypic and pathotypic diversity of Xanthomonas oryzae pv. oryzae, the cause of bacterial blight of rice in Punjab state of India. Journal of Phytopathology 159: 479-87
Manav, M. and Thind, B.S. (2002). Management of bacterial blight of rice with bioagents. Plant Disease Research 17: 21-28
Mew, T.W. and Rosales, A.M. (1986). Bacterization of rice plants for control of sheath blight caused by Rhizoctonia solani. Phytopathology 76: 1260–1264
Niranjana, S.R., Lalitha, S. and Hariprasad P. (2009). Mass multiplication and formulations of biocontrol agents for use against fusarium wilt of pigeonpea through seed treatment. Int. J. Pest Mgt. 55: 317-324
Notz, R., Maurhofer, M., Schnider-Keel, U., Duffy, B., Haas, D. and Defago, G. (2001). Biotic factors affecting
expression of the 2,4-diacetylphloroglucinol biosynthesis gene phlA in Pseudomonas fluorescens strain CHAO in the rhizosphere. Phytopathology 91: 873–881
Ou, S.H. (1973). A Handbook of Rice Diseases in the Tropics. Pp 58. Los Baños: International Rice Research Institute.
Papavizas, G.C. and Lewis, J.A. (1983). Physiological biocontrol characteristics of stable mutants of
Trichoderma viride resistance to MBC fungicides. Phytopathology, 73: 407–411
Raaijmakers, J.M., Bonsall, R.F. and Weller, D.M. (1999). Effect of population density of Pseudomonas
fluorescens on production of 2,4-diacetylphloroglucinol in the rhizosphere of wheat. Phytopathology 89: 470–475
Raaijmakers, J.M., Weller, D.M. and Thomashow, L.S. (1997). Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Applied and Environmental Microbiology 63: 881–887
Ramanamma, C., Bharathi, G. and Sreeramula, A. (1994). Paddy rhizosphere bacterium inhibiting rice plant pathogens. Journal of Ecotoxicology and Environmental Monitoring 4: 65-69
Rangarajan, S., Saleenal, L.M., Vasudevan, P. and Nair, S. (2003). Biological suppression of rice diseases by
Pseudomonas spp. under saline soil conditions. Plant and Soil 251: 73-82
Rishnamurthy, S. and Gnanamanickam, S.S. (1998) Biocontrol of rice sheath blight with formulated Pseudomonas putida. Indian Phytopathology 51: 233-36
Rodriguez, F. and Pfender, W.F. (1997). Antibiosis and antagonism of Sclerotinia homo eocarpa and Drechslera poae by Pseudomonas fluorescens PF-5 in vitro and in planta. Phytopathology 87: 614–621
Sakthivel, N., Swamani, E., Anuratha, C.S., Savithiry, S. and Gnanamanickam, S.S. (1988). Advances in research on plant pathogenic bacteria. In S.S. Gnanamanickam and A.J. Mahadevar (Eds.) Proceedings of National Symposium on Phytobacteriology held at the University of Madras, Madras, India, March 14-15, 1986 (pp. 213-220) New Delhi: Today and Tomorrow’s Printers and Publishers.
Santhi, D.P., Unnamalai, N. and Gnanamanickam, S.S. (1987). Epiphytic association of Erwinia herbicola with rice leaves infected by Xanthomonas oryzae pv. oryzae and its interaction with pathogen. Indian Phytopatholog 40: 327-32
Scarpellini, M., Franzetti, L. and Galli, A. (2004). Development of PCR assay to identify Pseudomonas fluorescens and its biotype. FEMS Microbiology Letters 236:257–260
Sharifi-Tehrani, A., Zala, M., Natsch, A., Moenne-Loccoz, Y. and Defago, G. (1998). Biocontrol of soil-borne fungal plant diseases by 2,4-diacetylphloroglucinol producing fluorescent pseudomonads with different restriction profiles of amplified 16S rDNA. European Journal of Plant Pathology 104: 631–643
Thakur, A., Singh, P.P. and Hunjan, M.S. (2011). Rhizosphere competence of Pf-4-R, a strain of Pseudomonas fluorescens colonizing rice roots. Plant Disease Research 26: 127-131
Thomashow, L.S., and Weller, D.M. (1995). Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites. In G. Stacey and N. Keen (Eds.) Plant-microbe interactions, Vol. 1 (pp. 187-235) New York: Chapman & Hall.
Velusamy, P., Defago, G., Thomashow, L. and Gnanamanickam, S.S. (2004). Role of 2,4-diacetylphloroglucinol for plant disease control: its importance to rice bacterial blight suppression in India. In L.V. Gangawane and C.D. Mayee (Eds.) Biotechnological approach for the integrated management of crop diseases (pp. 182-191) New Delhi: Daya Publishing House.
Velusamy, P., Immanuel, J.E., Gnanamanickam, S.S. and Thomashow, L. (2006). Biological control of rice
bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol. Can J Microbiol. 52:56-65.
Verma, K.K. (2009). Management of Meloidogyne javanica by bacterial antagonist Pseudomonas fluorescens as seedling root dip in tomato. Indian J. Nematol. 39: 207-210
Weller, D.M. (1983). Colonization of wheat roots by a fluorescent pseudomonad suppressive to take-all. Phytopathology 73:1548-1553
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Identification and characterization of Pseudomonas fluorescensstrains effective against Xanthomonasoryzaepv. oryzae causing bacterial blight of rice in Punjab, India. (2017). Journal of Applied and Natural Science, 9(1), 253-261. https://doi.org/10.31018/jans.v9i1.1181
