Adesh Kumar Tanjeet Singh Chahal Mandeep Hunjan Singh Singh Harminder Kaur Roomi Rawal


Alternaria black spot of pomegranate caused by Alternaria alternata pose significant economic losses in India as it reduce the crop yield. Farm survey was undertaken at Punjab Agricultural University, Pomegranate Research Block, Fruit Research Station, Jallowal- Lesriwal Jalandhar during 2015. Among all the five cultivars highest disease incidence (70%) and severity (30%) was registered in cultivar Bhagwa. Twenty two isolates of A. alternata were recovered from infected fruits and clustered using UPGMA (unweighted pair group method with arithmetic averages) on the basis of disease score produced in five cultivars (Ganesh, Ruby, Bhagwa, Jyoti and Mridula). It was revealed that five isolates namely AL14, AL15, AL20, AL21 and AL22 were highly virulent on almost all the pomegranate cultivars. Two cultivars viz. Bhagwa and Mridula were found to be most susceptible as 45.45 % isolates were found to be highly virulent on them.Thein vitro antifungal effects of the six fungicides on mycelial growth were investigated. Based on the inhibition of mycelial growth, all the fungicides showed most toxic reaction with 50% effective concentrations (EC50) of < 1 ppm. However, they varied in EC90 values. 10 ppm cocentration of Tilt (azole group) showed 90% effective concentration (EC90). Folicur also showed approximately same results as Tilt while EC90 value of Natio and SAAF was <25 ppm and > 20 ppm. However, two fungicides namely Dithane Z-78 and Bavistin showed less toxicity against Alternaria alternata as compared to other with EC90 value at concentrations <50 ppm and > 25 ppm. Azole group fungicides (Tilt and Folicur) were found most effective to inhibit the pathogen growth.


Download data is not yet available.


Metrics Loading ...




Alternaria black spot, Alternaria alternata, Cultivars, Fungicides, Pomegranate

Anonymous, (2015). Area and Production of pomegranate fruit in India. National horticulture mission online sources.
Archana, B.C. and Jamadar, M.M. (2014). Management of leaf spot and fruit spot /rot of pomegranate (Punica granatum L.) caused by Alternaria alternata (Fr.) Keissler. Karnataka J. Agric. Sci., 27:247-49.
Berbegal, M., López-Cortés, I., Salazar, D., Gramaje, D., Pérez-Sierra, A., García-Jiménez, J. and Armengol, J. (2014). First Report of Alternaria Black Spot of Pomegranate Caused by Alternaria alternatain Spain. Pl. Dis., 98:689
Ezra, D., Gat, T., Skovorodnikova, Y., Vardi, Y. and Kosto, I. (2010). First report of Alternaria black spot of pomegranate by Alternaria alternata in Israel. Aust. Plant Dis. Notes, 5:1-2
Farr, D. F., Rossman, A.Y., Palm, M. E. and McCray, E. B. (2007). Fungal Databases, Systematic Botany & Mycology Laboratory, USDA: ARS. Retrieved February 5, 2007, from http://nt.ars-grin.gov/fungaldatabases.
Gat, T., Liarz, I. O., Skovorodnikova, Y., and Ezra, D, (2012). Characterization of Alternaria alternata causing black spot disease of pomegranate in Israel using a molecular marker. Plant Dis., 96:1513-18
Holland, D., Hatib, K. and Bar-Ya’akov, I. (2009). Pomegranate: botany, horticulture, breeding. Hortic. Rev., 35:127-92
Islam, S.S., Rahman, M. H., Hasan, M. J., Ashadusjaman, M. and Khatun, M.M. (2007). Efficacy of fungicidal seed treatment in Controlling Alternaria spp. in Radish Seed. Int. J. Sustain Crop Prod., 2:46-50
Ito, K., Tanaka, T., Hatta, R., Tamamoto, M., Akimitsu, K. and Tsuge, T. ( 2004). Dissection of the host range of the fungal plant pathogen Alternaria alternata by modification of secondary metabolism. Mol. Microbiol., 52:399-411
Ivić, D., Sever, Z. and Kuzmanovska, B. 2011. In vitro sensitivity of Fusarium graminearum, F. avenaceum and F. verticillioi-des to carbendazim, tebuconazole, flutriafol, metconazole and prochloraz. Pestic. Phytomed., 26:35-42
Koller, W. and Scheinpflug, H. (1987). Fungal resistance to sterol biosynthesis inhibitors: a new challenge. Plant Dis., 71:1066-74
Madhukar, J. and Reddy, S. M. (1976). Some new leaf spot diseases of pomegranate. Indian J. Mycol. Plant Path.,18:171–72
Matheron, M.E. and Porchas, M. (2000). Impact of azoxystrobin, dimethomorph, fluazinam, fosetyl-al, and metalaxyl on growth, sporulation, and zoospore cyst germination of three Phytophthora spp. Plant Dis., 84:454-58
Mesta, R.K., Benagi V. I., Kulkarni, S. and Basavarajappa, M.P. (2011). Management of Alternaria blight of sunflower through fungicides. Karnataka J. Agric. Sci., 24: 149-152
Nel, B., Steinber, C., Labuschagne, N. and Viljoen, A. (2007). Evaluation of fungicides and sterilants for potential applica-tion in the management of Fusarium wilt of banana. Crop Prot., 26:697-705
Pala, H., Tatli, A., Yilmaz, C. and Özgüven, A.I. (2009). Important disease of pomegranate fruit and control possibilities in Turkey. Acta. Hortic., 818:285-90
Pasche, J. S., Wharam, C. M. and Gudmestad, N. C. (2004). Shift in sensitivity of Alternaria solani in response to QoI fungicides. Plant Dis., 88:181-187
Reuveni, M.S.D. (2002). Effects of azoxystrobin, difenoconazole, polyoxin B (polar) and trifloxystrobin on germination and growth of Alternaria alternata and decay in red delicious apple fruit. Crop Protec., 21:951-55
Shin, J.H., Han, J.H., Lee, J.K. and Kim, K.S. (2014). Characterization of the maize stalk rot pathogens Fusarium subglutinans and F. temperatum and the effect of fungicides on their mycelial growth and colony formation. Plant Pathol. J., 30:397-406
Simmons, E.G. (1967). Typification of Alternaria, Stemphylium and Ulocladium. Mycologia, 59:67–92
Tziros, G.T., Lagopodi, A.L. and Tzavella-Klonari, K. (2007). Alternaria alternata fruit rot of pomegranate (Punica granatum) in Greece. New Dis. Rep., 15:14
Yin, Y., Liu, X., Li, B. and Ma, Z. (2009). Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asi-aticum and F. graminearum collected from wheat in China. Phytopathology, 99:487-497
Citation Format
How to Cite
Kumar, A., Chahal, T. S., Singh, M. . H. S., Kaur, H., & Rawal, R. (2017). Studies of alternaria black spot disease of pomegranate caused by Alternaria alternata in Punjab. Journal of Applied and Natural Science, 9(1), 156-161. https://doi.org/10.31018/jans.v9i1.1164
More Citation Formats:
Research Articles