The root rot disease in Jatropha curcas L. caused by Rhizoctonia. bataticola (Taub.) Butler has been recorded in causing 10-12 per cent mortality of 20-30 days old seedlings of Jatropha curcasin southern Haryana. The incidence of this disease has also been observed from other parts of Haryana too. Induction of systemic resistance in host plants through microbes and their bioactive metabolites are attaining popularity in modern agricultural practices. Studies on the plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola were undertaken at Chaudhary Charan Singh, Haryana Agricultural University, Regional Research Station, Bawal. Three plant growth-promoting rhizobacteria (PGPRs) viz., Pseudomonas maltophila, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their potential to induce systemic resistance in Jatropha against root rot. The maximum increase of 97 per cent in total phenols, 120 per cent in peroxidase, 123 per cent in polyphenol oxidase, 101 per cent in phenylalanine ammonia lyase and 298 per cent in tyrosine ammonia lyase was detected in plants raised with Pseudomonas fluorescens+ Rhizoctoniaba-taticola inoculation in Jatropha curcas at 10 days post inoculation against control except total phenols where it was maximum (99%) at 30 DPI. There was slight or sharp decline in these parameters with age irrespective of inoculations. The pathogen challenged plants showed lower levels of total phenols and enzymes. The observations revealed that seed bacterization with Pseudomonas fluorescens results in accumulation of phenolics and battery of enzymes in response to pathogen infection and thereby induce resistance systemically.
Jatropha curcas root rot, Phenol oxidizing enzymes, Phenol synthesizing enzymes, Rhizoctoniabataticola
Dixon, R.A. and Harrison, M.J. (1990). Activation structure and organization of genes involved in microbial defence in plants. Advance Gen., 8: 165-234
George, W.S. and Willium, G.C. (1957). Statistical methods (6th ed.). Oxford and IBH Publishing Company. pp. 541-573
Ghosh, R. (2015). Enzymatic responses of ginger plants to Pythium infection after SAR induction. J. Plant Pathol. Microb., 6: 283
Joshi U.N., Gupta P.P., Gupta, U. and Kumar, S. (2004). Biochemical factors in clusterbean that impact Alternaria blight resistance. J. Mycol. Pl. Pathol., 34: 581-583
Kenten, R. H. (1956). Latent phenolase in extracts of broadbean (Vicia faba L.) leaves. Biochem. J., 68: 244-251
Kosuge, T. (1969). The role of phenolics in host response to infection. Ann. Rev. Phytopathol., 7: 195-222
Kumar, S., Sharma, S., Pathak, D.V. and Beniwal, J. (2011). Integrated management of Jatropha root rot caused by Rhizoctonia bataticola. J. Trop. Forest. Sci., 23: 35-41
Kumar, Vinod., Kumar, A., Verma, V.C., Gond, S.K., and Kharwar, R.N. (2007). Induction of defense enzymes in Pseudomonas. fluorescens treated chickpea roots against Macrophomina phaseolina. Indian Phytopath., 60: 289-295
Liang, Jian-Gen., Tao, Rong-Xiang., Hao, Zhong-na., Wang,Lian-ping and Zhang, Xin. (2011). Induction of resistance in cucumber against seedling damping-off by plant growth-promoting rhizobacteria (PGPR) Bacillus megaterium strain L8. African J. Biotechnol., 10: 6920 6927
Mahadevan, A. and Sridhar R. (1982). Methods in Physiological Plant Pathology. II. Ed. Sivakami Publications, Madras, India.
Michel, Shoresh., Yedidia, Iris. and Chet Han. (2005). Involvement of Jasmonic acid/Ethylene signaling
pathway in the systemic resistance induced in cucumber by Trichoderma asperellum T 203. Phytopathology, 95: 76-84
Mishra, A.K., Morang, P., Deka, M., Nishant Kumar, S. and Dileep Kumar, B.S. (2014). Plant growth-promoting rhizobacterial strain-mediated induced systemic resistance in tea (Camellia sinensis (L.) O. Kuntze) through defense-related enzymes against brown root rot and charcoal stump rot. Appl. Biochem. Biotechnol., 174: 506-521
Salari, Moammad., Panjekeh, Naser., Nasirpoor, Zahrah and Abkhoo, Javad. (2012). Changes in total phenol, total proptein and peroxidase activities in melon (Cucumis melo L.) cultivars inoculated with Rhizoctonia solani. African J. Microbio. Res., 37: 6629-6634
Sharma, I.M. (2003). Relationship between phenylpropanoid metabolism and resistance to Pythium ultimum Trow. in apple rootstock. J. Mycol. Pl. Pathol. 33: 114-115
Sharma, Sushil and Kumar, Krishan. (2009). Root rot of Jatropha curcas incited by Rhizoctonia bataticola in India. Indian Forester, 135: 433-434
Sreedevi, Basavaraju and Charithadevi, Mekala. (2012). Mechanism of biological control of root rot of groundnut caused by Macrophomina phaseolina. Indian Phytopath., 65: 360-365
Vidhyasekran, P. (1997). Fungal pathogenesis in plants and crop molecular biology and host defence mechanism. Marck Dekker, New York, USA. 553pp.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)