S. R. Parida S. Beura S. Rout R. Beura P. N. Jagadev


An investigation was conducted on Fast Protocol for High Frequency in vitro cloning of Banana (Musa acuminata) cv. Grande Naine at the Biotechnology-cum-Tissue Culture Center, OUAT, Bhubaneswar, during the year 2012. This has helped to determine the best media compositions for shoot multiplication and rooting of cv. Grande Naine, so as to get optimum results with a minimized cost of production. MS medium supplemented with 4.0 mg/1 Benzylaminopurine (BAP) and 2.0 mg/1 Kinetin gave the highest number of shoot/explants (11.33) in 30 days. However, MS medium when supplemented with 6.0 mg/1 BAP produced a maximum number of leaves (19.07) with a maximum height 2.73 cm. Among various concentrations of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Half MS medium supplemented with 1.0 mg/1 IBA was found to be ideal for early rooting and producing more number of roots in 21 days. However, MS basal medium was found to be the best treatment to support the formation of long roots. This protocol can be very useful to the future research worker and as well as entrepreneurs for mass production of banana (Musa acuminata) cv. Grande Naine.


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In vitro, inoculation, Musa acuminate, Shoot proliferation

Al-amin, M., Karim, M., Amim, M., Rahaman, S. and Mamun, A.N.M.(2009). In vitro micropropagation of banana (Musa spp.). Bangladesh Jour. Agri. Res. 34(4):645-659
Ammirato PV (1982). Growth and morphogenesis in cultures of the monocot yam, Dioscorea. In: Plant Tissue
Culture, Fugiwara A (Ed.), Maruzen,Tokyo, Japan, pp. 169- 170
Arias, O. (1992). Commercial micropropagation of banana. In: Biotechnology Applications for Banana and Plantain Improvement. lnibap, San Jose, Costa Rica. pp. 139-142
Arvanitoyannis, I.S., Mavromatis, A.G., Grammatikaki-Avgeli, O. and Sakellariou, M. (2007). Banana: Cultivars, biotechnological approaches and genetic transformation. Int. Jour. of Food Science and Technology. 43:1871-1879
FAO. (2005). ProdSTAT: Crops. Food and Agriculture Organization. United Nations. :9-12.
Gomez, K.A. and Gomez, A.A .(1984). Completely Randomized Design. In: Statistical procedures for
Agricultural Research, 2ndEdn. John Wiley and Sons, New York, 8-20
Haq, I. and Dahot, M.U. (2007). Micropropagation efficiency in banana (Musa sp.) under different
immersion systems. Pakistan Journal of Biological Sciences. 10(5):726-733
Hussain, C.T.S. (1995). Response of gladiolus to rapid cloning through in vitro techniques. M.Sc thesis Kerala Agric, Univ., vellanikkara, Thrissur.
Hussey G. (1975). Totipotency in tissue explant and callus of some members of Liliaceae, Iridaceae and amaryllidaceae. J. Exp. Bot., 26 (91): 253-262
Lentfer, C. and Boyd, W. (2007). "Tracing antiquity of banana cultivation in Papua New Guinea". The Australia & Pacific Science Foundation. A project undertaken at the Centre for Geoarchaeology and Palaeoenvironmental Research, School of Environmental Science and Management, Southern Cross University, Lismore, NSW.
Meenakshi, N., Sulikeri, G.S., Krishnamoorthy, V. and Hegde, R.V. (2001). Standardization of chemical environment for multiple shoot induction of turmeric (Curcuma longa L.) for in vitro clonal propagation. Crop Research Hisar. 22(3), 449-453
Moore, T.C. (1989). Auxins. In: Biochemistry and Physiology of Plant Hormones. 2nd edn, 28- 93. Nurosa Publ. House. N. Delhi.
Murashige, T. (1974). Plant propagation through tissue culture. Annual Review of Plant Physiology. 25:135-166.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Physiologia Plantarum. 15(3):473-497
Palai, S.K. (2001). Studies on physiological, biochemical and molecular status in vitro culture of ginger (Zingiber Officinale Rosc ) and turmeric (Curcuma longa L.) Ph.D thesis, Utkal University.
Palai, S.K., Rout, G.R., Das, P. and Edison, S. (1997). Micropropagation of ginger. Interaction of growth regulator and culture condition. Proceedings of the National Seminar on Biotechnology of Spices and Aromatic Plants. (Eds. Raman KV, Sasikumar B, Babu KN, Eapen SJ) Calicut, pp. 20.
Rahman, M.Z., Nasiruddin, K.M., Amin, M.A. and Islam, M.N. (2004). In vitro response and shoot multiplication of Banana with BAP and NAA. Asian Journal of Plant Sciences. 3(4): 406-409
Randy, C.P. (2007). Banana and plantain – an overview with emphasis on pacific island cultivars species profiles for pacific island agro forestry ver, 1
Rout, G.R., Palai, S.K., Samantaray, S. and Das, P. (1995). Metabolic changes during in vitro multiplication of Curcuma longa cv. Surama and PTS-28. Acta Botanica Hungarica. 39(3-4): 383-392
Rubery, P.H. and Sheldrake, A.R. (1973). Effect of pH and surface charge on cell uptake of auxin.Natures (Lond.). New Biol. 244:285-288
Sahoo, C., Beura, S., Rout, S. and Beura. R.(2015). High frequency In vitro cloning of Banana (Musa acuminate) cv. Grande Naine. Int. Jour. of Agri., Environment and Biotechnology. 8(4):943-950
Tiwari. R.S. (1998). Micropropagation of turmeric (Curcuma longa L.). Recent horticulture. 4:145-148
Viehmannova, I., Fernandez, C.E., Hnilicka, F. and Robles, C.D. (2007). The influence of growth regulators on root induction In vitro of Musa genus. Agri. Tropica et Subtropica. 40 (3):115-119
Wang and Hu. (1980). In vitro clonal propagation of ginger sprouts. Acta Botanica Yannanica. 11, 231-233
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Parida, S. R., Beura, S., Rout, S., Beura, R., & Jagadev, P. N. (2017). Fast protocol for high frequency in vitro cloning of Banana (Musa acuminata) cv. Grande Naine. Journal of Applied and Natural Science, 9(1), 72–79. https://doi.org/10.31018/jans.v9i1.1153
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