Shabana Bano Nashrah Ahmad A. K. Sharma


The present study deals with the phytochemical screening and evaluation of antibacterial and antioxidant activities from the crude methanol extract of the seeds of cardamom, Elettaria cardamom. Crude methanol extract was investigated for their antibacterial activity against Enteropathogenic E. coli (EPEC), Listeria monocytogenes,
Bacillus pumilus and Escherichia coli. The extract showed maximum zone of inhibition (20.3 mm) against EPEC, however, the antibacterial potential of the extract was slightly lesser against normal E. coli (19 mm). It showed moderate anti-bacterial activity against L. monocytogenes and B. pumilus. Dose-dependent increase in antioxidant activity was also noticed in crude extract as measured by DPPH free radical scavenging assay. Thus, our study reports various phytochemicals in the seeds of cardamom with antioxidant and antibacterial potential.


Download data is not yet available.


Metrics Loading ...




Anti-microbial activity, Anti-oxidant activity, Cardamom, DPPH

Agarry, O.O., Olaleye, M.T. and Bello-Michael, C.O. (2005). Comparative antimicrobial activities of aloe vera gel and leaf. Afr. J. Biotechnol, 4(12): 1413-1414
Aiyelaagbe O.O. and Osamudiamen P.M. (2009). Phyto-chemical screening for active compounds in mangifera indica leaves from ibadan, Oyo State. Plant Sciences Research, 2: 11-13
Baliere C, Rince A, Delannoy S, Fach P. and Gourmelon M. (2016) .Molecular profiling of STEC and EPEC strains isolated from French coastal environments. Appl Environ. Microbiol., 22 : 271-16
Bhattacharjee, B. and Chatterjee J. (2013). Identification of proapoptopic, anti-inflammatory, anti- proliferative, anti-invasive and anti-angiogenic targets of essential oils in cardamom by dual reverse virtual screening and binding pose analysis. Asian Pac J Cancer Prev., 14(6):3735-42
Biswas, M., Haldar, P.K. and Ghosh, A.K. (2010) Antioxi-dant and free-radical-scavenging effects of fruits of Dregea volubilis, J Nat Sci Biol Med. Jul-Dec; 1(1): 29–34
Bruneton, J. (2001). Pharmacognosy, phytochemistry, me-dicinal plants. 2nd edition. Lavoisier Publishers. Paris, pp 83-90
Chawla, T., Abbasi, N. I. and Tandon, A. (2014). Antimicro-bial activity of spices like cloves Cardamom and Cinna-mon on Bacillus and Pseudomonas.International Journal of Drug Development and Research.
Chen C.W. and Ho C.T. (1995). Antioxidant properties of polyphenols extracted from green and black teas, Jour-nal of Food Lipids, 2 (1) : 35-46
Edeoga, H.O., Okwu, D.E. and Mbaebie, B.O. (2005). Phy-tochemical constituents of some Nigerian medicinal plants, Afr. J. Biotechnol, 4(7): 685-688
Grass G., Bierbaum, G., Molitor E., Gotte, N. and Antwer-pen, M. (2016) Genome sequence of bacillus pumilus strain bonn, Isolated from an Anthrax-Like Necrotic Skin Infection Site of a Child. Genome Announc, 11;4(1) : 1741-15
Hero, F., Akrayi, S. and Tikrit, P. (2012). Antibacterial effect of seed extracts of cardamom (Elettaria cardamomum) against staphylococcus aureus and proteus mirabilis. Journal of Pure Science, 17 (2) : 1813 – 1662
Islam S., Rahman A., Sheikh M., Rahman M., Mostofa, A.H. and Alam, F. (2010). In vitro antibacterial activity of methanol seed extract of elettaria cardamomum (L.) maton. Agriculturae Conspectus Scientifi cus., 75(3): 113-117
Jayawardena, N., Watawana, M.I., Jayathilaka, R.T. and Waisundara, V.Y. (2015). The antioxidant and starch hydrolase inhibitory activity of ten spices in an in vitro model of digestion: bioaccessibility of anthocyanins and carotenoids. Evid Based Complement Alternat Med., 10 : 764-773
Jebur M.H., Bnuyan, I., Yasri, A.A. and Kadhim, N.K. (2014). Antimicrobial effect of seed extracts, leaves and crude oil of cardamom (elettaria cardamomum) against different types of bacteria in hilla city, iraq, World Jour-nal of Pharmaceutical Research, (3): 4934-4944
Kapoor I.P.S., Singh, B., Singh, G., Valery A Isidorov, Lech Szczepaniak. (2008). Chemistry, antifungal and antioxi-dant activities of cardamom (Amomum subulatum) essential oil and oleoresins. Int. J. Essent. Oil Thera-peut, 2(1): 29-40
Khandelwal, K.R. (2001). Preliminary phytochemicals screening, Practical Pharmacognosy - Techniques and Experiments, 8th edn, pp-149-156
Kogure, K., Yamauchi, I., Tokumura A., Kondou K., Ta-naka, N., Takaishi, Y. and Fukuzawa, K. (2004). Novel antioxidants isolated from plants of the genera Ferula, Inula, Prangos and Rheum collected in Uzbekistan. Phytomedicine, 11(7-8):645-51
Mishra, N. and Behal, K.K. (2010). Antimicrobial activity of some spices against selected microbes, International Journal of Pharmacy and Pharmaceutical Science, 2:(3)187-196
Nair, S., Nagar, R. and Gupta, R. (1998). Antioxidant pheno-lics and flavonoids in common Indian foods. J Assoc Physicians India, 46: 708-10
Naveed, R. Hussain, I., Tawab, A., Tariq, M., Rahman, M., Hameed, S., Mahmood, M.S., Siddique, A.B., Iqbal, M. (2013). Antimicrobial activity of the bioactive compo-nents of essential oils from Pakistani spices against Salmonella and other multi-drug resistant bacteria. BMC Complement Altern Med. 14: 265-270
Obadoni, B.O. and Ochuko, P.O. (2001). Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Glob.J. Pure Appl. Sci., 86: 2003-2008
Shegarfi, H., Rolstad, B., Kane, K.P. and Nestvold, J. (2016). Listeria monocytogenes infection differentially affects expression of ligands for NK cells and NK cell re-sponses, depending on the cell type infected. J Leukoc Biol., 2: 1115-506
Srinivasan, D., Perumalsamy, L.P. and Suresh, T. (2001). Antimicrobial activity of certain Indian medicinal plants used in folk medicine. J. Ethnopharm, 1 (7): 94-99
Sultana, S., Ripa, F.A. and Hamid, K. (2010). Comparative antioxidant activity study of some commonly used spices in Bangladesh. Pakistani Journal of Biological Sciences, 1:13(7): 340-343
Umamaheswari, M. and Chatterjee, T.K. (2008). In vitro antioxidant activities of the fractions of Coccinia Grandis L. leaf extract. Afr. J Trad. Com.p Alter. Med., 5:61–73
Citation Format
How to Cite
Bano, S., Ahmad, N., & Sharma, A. K. (2016). Phytochemical screening and evaluation of anti-microbial and anti-oxidant activity of Elettaria cardamom (Cardamom). Journal of Applied and Natural Science, 8(4), 1966–1970. https://doi.org/10.31018/jans.v8i4.1071
More Citation Formats:
Research Articles