Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni
Article Main
Abstract
In the present investigation, the dynamics of steviol glycosides (stevioside and rebaudioside-A) of Stevia rebaudiana with their growth stages were studied. The study aimed to examine the best stage of harvesting (month of the year) the crop with respect to maximum accumulation of stevioside and rebaudioside-A content in different plant parts (leaves, green stem and woody stem). The results showed that the maximum stevioside content in leaves (8.55%) was found in June month (vegetative stage). Rebaudioside-A content in leaves (7.00%) was at its peak in August (vegetative stage). Whereas, higher stevioside and rebaudioside-A content was found for green stem (0.93%) and woody stem (0.18%) during September month (flowering stage). Leaves showed maximum yields of stevioside (17.60g) and rebaudioside-A (13.75g) per plant in July month. The study indicated that it is economical to harvest the leaves of S. rebaudiana rather than harvesting whole aerial biomass in vegetative phase (July month).
Article Details
Article Details
Keywords
Fresh and dry weights, Plant growth, Stevia rebaudiana, Stevioside and rebaudioside-A
References
Arya, A., Kumar, S. and Kasana, M.S. (2012). Anti-inflammatory activity of in vitro regenerated calli and in vivo plant of Stevia rebaudiana (Bert.) Bertoni. Interna-tional Journal of Scientific and Research Publications, 2: 1-5
Bondarev, N.I., Sukhanova, M.A., Reshetnyak, O.V. and Nosov, A.M. (2003). Steviol glycoside content in different organs of Stevia rebaudiana and its dynamics during ontogeny. Biologia Plantarum, 47: 261-264
Ceunen, S. and Geuns J.M.C. (2013). Spatio-temporal varia-tion of the diterpene steviol in Stevia rebaudiana grown under different photoperiods. Phytochemistry, 89: 32-38
Ceunen, S., Werbrouck, S. and Geuns, J.M.C. (2012). Stimulation of steviol glycoside accumulation in Stevia rebaudiana by red LED light. Journal of Plant Physiology, 169: 749–752
Chalapathi, M.V. and Thimmegowda, S. (1997). Natural non-calorie sweetener stevia (Stevia rebaudiana Bertoni): a future crop of India. Crop Research Hisar, 14: 347-350.
Fujita, H. and Edahiro, T. (1979). Safety and utilization of stevia sweetener. Food Industry, 22: 65-72
Gardana, C., Scaglianti, M. and Simonetti, P. (2010). Evalua-tion of steviol and its glycosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high performance liquid chromatography–mass spectros-copy. Journal of Chromatography A, 1217: 1463–1470
Ghanta, S.A., Banerjee, A., Podder and Chattopadhyay, S. (2007). Oxidative DNA damage preventive activity and anti oxidant potential of Stevia rebaudiana (Bertoni) a natural sweetener. Journal of Agricultural and Food Chemistry, 55: 962-967
Gupta, E., Purwar, S., Sundaram, S. and Rai, G. K. (2013). Nutritional and therapeutic values of Stevia rebaudiana: a review. Journal of Medicinal Plants Research, 46: 3343-3343
Hseih, M.H., Chan, P., Sue, Y.M., Liu, J.C., Liang, T.H., Huang, T.Y., Tomlinson, B., Chow, M.S., Kao, K.F. and Chen, Y.I. (2003). Efficacy and tolerability of oral stevioside in patients with mild essential hypertension: a two year randomized, placebo-controlled study. Clinical Therapeutics, 25: 2797-2808
Jeppesen, P.B., Gregersen, S., Alstrup, K.K. and Hermansen, K. (2002). Stevioside induces antihyperglycaemic, insulinotropic and glucagonostatic effects in vivo: studies in the diabetic Goto-Kakizaki (GK) rats. Phytomedicine, 9: 9-14
Kaushik, R., Narayanan, P., Vasudevan, V., Muthukumaran, G. and Antony, U. (2010). Nutrient composition of cultivated stevia leaves and the influence of polyphe-nols and plant pigments on sensory and antioxidant properties of leaf extracts. Journal of Food Science and Technology, 47: 27-33
Kennelly, E.J. (2002). Sweet and non-sweet constituents of Stevia rebaudiana (Bertoni). Bertoni. In: Stevia, the genus Stevia: Medicinal and Aromatic plants – Indus-trial profiles vol. 19. (ed. Kinghorn, A. D.). London: Taylor and Francis, pp. 68-85.
Kinghorn, A.D. and Soejarto, D.D. (1985). Current status of stevioside as a sweetening agent for human use. In: Economic and medicinal plant research. vol.1. (eds. Wagner, H., Hikino, H. and Farnsworth, N. R.). London, New York Academic Press, pp. 1-52
Misra, H., Mehta, D., Mehta, B.K. and Jain, D.C. (2015). Antihypertensive (blood pressure lowering) effects of stevioside, from Stevia rebaudiana Bertoni, on rats, dogs and humans- A short review. Indian Journal of Drugs, 3: 102-108
Nakamura, S. and Tamura, Y. (1985). Variation in the main glycosides of Stevia (Stevia rebaudiana Bertoni). Japanese Journal of Tropical Agriculture, 29: 109-116
Serfaty, M., Ibdah, M., Fischera, R., Chaimovitsha, D., Sarangab, Y. and Dudaia N. (2013). Dynamics of yield components and stevioside production in Steviarebaudi-ana grown under different planting times, plant standsand harvest regime. Industrial Crops and Products, 50 731– 736
Shivanna, N., Naika, M., Khanum, F. and Kaul, V.K. (2013). Antioxidant, antidiabetic and renal protective properties of Stevia rebaudiana. Journal of Diabetes and its Complications, 27: 103-113
Staratt, A.N., Kirby, C.W., Pocs, R. and Brandle, J.E. (2002). Rebaudioside E, a diterpene glycoside from Stevia rebaudiana. Phytochemistry, 59: 367-370
Takahashi, K., Sun, Y., Yanagiuchi, I., Hosokawa, T., Saito, T., Komori, M., Okino, T. and Kurasaki, M. (2012). Stevioside enhances apoptosis induced by serum deprivation in PC1 2 cells. Toxicology Mechanisms and Methods, 22: 243- 249
Tavarini, S. and Angelini, L.G. (2013). Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop aglycon steviol glycoside content and antioxidant properties. Journal of the Science of food and Agriculture, 93: 2121–2129
Terai, T., Ren, H., Mori, G., Yamaguchi,Y. and Hayashi, T. (2002). Mutagenicity of steviol and its oxidative deriva-tive in Salmonella typhimurium TM677. Chemical and Pharmaceutical Bulletin, 50: 1007-1010
Zaidan, L.B.P., Dietrich, S.M.C. and Felippe, G.M. (1980). Effect of photoperiod on flowering and stevioside content in plants of Stevia rebaudiana Bertoni. Japanese Journal of Crop Science, 49: 569-574
Bondarev, N.I., Sukhanova, M.A., Reshetnyak, O.V. and Nosov, A.M. (2003). Steviol glycoside content in different organs of Stevia rebaudiana and its dynamics during ontogeny. Biologia Plantarum, 47: 261-264
Ceunen, S. and Geuns J.M.C. (2013). Spatio-temporal varia-tion of the diterpene steviol in Stevia rebaudiana grown under different photoperiods. Phytochemistry, 89: 32-38
Ceunen, S., Werbrouck, S. and Geuns, J.M.C. (2012). Stimulation of steviol glycoside accumulation in Stevia rebaudiana by red LED light. Journal of Plant Physiology, 169: 749–752
Chalapathi, M.V. and Thimmegowda, S. (1997). Natural non-calorie sweetener stevia (Stevia rebaudiana Bertoni): a future crop of India. Crop Research Hisar, 14: 347-350.
Fujita, H. and Edahiro, T. (1979). Safety and utilization of stevia sweetener. Food Industry, 22: 65-72
Gardana, C., Scaglianti, M. and Simonetti, P. (2010). Evalua-tion of steviol and its glycosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high performance liquid chromatography–mass spectros-copy. Journal of Chromatography A, 1217: 1463–1470
Ghanta, S.A., Banerjee, A., Podder and Chattopadhyay, S. (2007). Oxidative DNA damage preventive activity and anti oxidant potential of Stevia rebaudiana (Bertoni) a natural sweetener. Journal of Agricultural and Food Chemistry, 55: 962-967
Gupta, E., Purwar, S., Sundaram, S. and Rai, G. K. (2013). Nutritional and therapeutic values of Stevia rebaudiana: a review. Journal of Medicinal Plants Research, 46: 3343-3343
Hseih, M.H., Chan, P., Sue, Y.M., Liu, J.C., Liang, T.H., Huang, T.Y., Tomlinson, B., Chow, M.S., Kao, K.F. and Chen, Y.I. (2003). Efficacy and tolerability of oral stevioside in patients with mild essential hypertension: a two year randomized, placebo-controlled study. Clinical Therapeutics, 25: 2797-2808
Jeppesen, P.B., Gregersen, S., Alstrup, K.K. and Hermansen, K. (2002). Stevioside induces antihyperglycaemic, insulinotropic and glucagonostatic effects in vivo: studies in the diabetic Goto-Kakizaki (GK) rats. Phytomedicine, 9: 9-14
Kaushik, R., Narayanan, P., Vasudevan, V., Muthukumaran, G. and Antony, U. (2010). Nutrient composition of cultivated stevia leaves and the influence of polyphe-nols and plant pigments on sensory and antioxidant properties of leaf extracts. Journal of Food Science and Technology, 47: 27-33
Kennelly, E.J. (2002). Sweet and non-sweet constituents of Stevia rebaudiana (Bertoni). Bertoni. In: Stevia, the genus Stevia: Medicinal and Aromatic plants – Indus-trial profiles vol. 19. (ed. Kinghorn, A. D.). London: Taylor and Francis, pp. 68-85.
Kinghorn, A.D. and Soejarto, D.D. (1985). Current status of stevioside as a sweetening agent for human use. In: Economic and medicinal plant research. vol.1. (eds. Wagner, H., Hikino, H. and Farnsworth, N. R.). London, New York Academic Press, pp. 1-52
Misra, H., Mehta, D., Mehta, B.K. and Jain, D.C. (2015). Antihypertensive (blood pressure lowering) effects of stevioside, from Stevia rebaudiana Bertoni, on rats, dogs and humans- A short review. Indian Journal of Drugs, 3: 102-108
Nakamura, S. and Tamura, Y. (1985). Variation in the main glycosides of Stevia (Stevia rebaudiana Bertoni). Japanese Journal of Tropical Agriculture, 29: 109-116
Serfaty, M., Ibdah, M., Fischera, R., Chaimovitsha, D., Sarangab, Y. and Dudaia N. (2013). Dynamics of yield components and stevioside production in Steviarebaudi-ana grown under different planting times, plant standsand harvest regime. Industrial Crops and Products, 50 731– 736
Shivanna, N., Naika, M., Khanum, F. and Kaul, V.K. (2013). Antioxidant, antidiabetic and renal protective properties of Stevia rebaudiana. Journal of Diabetes and its Complications, 27: 103-113
Staratt, A.N., Kirby, C.W., Pocs, R. and Brandle, J.E. (2002). Rebaudioside E, a diterpene glycoside from Stevia rebaudiana. Phytochemistry, 59: 367-370
Takahashi, K., Sun, Y., Yanagiuchi, I., Hosokawa, T., Saito, T., Komori, M., Okino, T. and Kurasaki, M. (2012). Stevioside enhances apoptosis induced by serum deprivation in PC1 2 cells. Toxicology Mechanisms and Methods, 22: 243- 249
Tavarini, S. and Angelini, L.G. (2013). Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop aglycon steviol glycoside content and antioxidant properties. Journal of the Science of food and Agriculture, 93: 2121–2129
Terai, T., Ren, H., Mori, G., Yamaguchi,Y. and Hayashi, T. (2002). Mutagenicity of steviol and its oxidative deriva-tive in Salmonella typhimurium TM677. Chemical and Pharmaceutical Bulletin, 50: 1007-1010
Zaidan, L.B.P., Dietrich, S.M.C. and Felippe, G.M. (1980). Effect of photoperiod on flowering and stevioside content in plants of Stevia rebaudiana Bertoni. Japanese Journal of Crop Science, 49: 569-574
Issue
Section
Research Articles
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
How to Cite
Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni. (2016). Journal of Applied and Natural Science, 8(4), 1953-1958. https://doi.org/10.31018/jans.v8i4.1069
