Serological detection of tomato yellow leaf curl virus in different parts of tomato through DAS-ELISA revealed that its branches have highest virus concentration followed by twigs, mature leaves and middle leaves. The samples derived from the branches gave highest mean OD value 0.517 whereas, the recorded mean OD values in case of twigs, mature leaves and middle leaves were 0.470, 0.444 and 0.419, at A405 nm, respectively. The virus was not found to be present in flowers and fruits as indicated by lower mean OD values, i.e. 0.215 and 0.159, respectively. On the basis of findings of the our experiment, the branches, twigs, mature leaves and middle leaves had high concentration of the virus and hence these parts could be used in the detection of TYLCV, whereas, flowers and fruits had very low titer of virus, thus, not recommended to be used for serological detection.
Optical density, Serology, Tomato, Tomato yellow leaf curl virus
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