Distribution of tomato yellow leaf curl virus (TYLCV) in different parts of tomato plants: A serological perspective
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Abstract
Serological detection of tomato yellow leaf curl virus in different parts of tomato through DAS-ELISA revealed that its branches have highest virus concentration followed by twigs, mature leaves and middle leaves. The samples derived from the branches gave highest mean OD value 0.517 whereas, the recorded mean OD values in case of twigs, mature leaves and middle leaves were 0.470, 0.444 and 0.419, at A405 nm, respectively. The virus was not found to be present in flowers and fruits as indicated by lower mean OD values, i.e. 0.215 and 0.159, respectively. On the basis of findings of the our experiment, the branches, twigs, mature leaves and middle leaves had high concentration of the virus and hence these parts could be used in the detection of TYLCV, whereas, flowers and fruits had very low titer of virus, thus, not recommended to be used for serological detection.
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Article Details
Optical density, Serology, Tomato, Tomato yellow leaf curl virus
Anonymous. (2015). Handbook of indian horticulture data-base, NHB, Gurgaon. department of agriculture and cooperation, Ministry of Agriculture, Government of India, New Delhi. pp 48
Cerkauskas, R. F. (2005). Tomato disease-Tomato Yellow Leaf Curl Virus (TYLCV). Asian Vegetable Research and Development Center Report, AVRDC Publication, No. 04-610.
Clark, M.F. and Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34: 475-483
Czosnek, H. (2008). Tomato yellow leaf curl Virus. Encyclo-pedia of Virology, Vol. 5, Elsevier, Oxford, pp 138-145
Czosnek, H. and Laterrot, H. (1997). A world wide survey of tomato yellow leaf curl virus. Archives of Virology, 142: 1391-1406
Devaraja, Narayanaswamy, K., Savithri, H.S. and Muniyappa, V. (2005). Purification of tomato leaf curl Bangalore virus and production of polyclonal antibod-ies. Current Science, 89(1): 181-183
Green, S.K. and Kalloo, G. (1994). Leaf curl and yellowing viruses of pepper and tomato: An overview. AVRDC Tech. Bull. No 21
Kumar, S. and Srivastava, K. (2013). Screening of tomato genotypes against early blight (Alternaria solani) under field condition. The Bioscan, 8(1): 189-193
Kushwaha, N., Singh, A.K., Chattopadhyay, K. and Chak-raborty, S. (2010). Recent advances in geminivirus de-tection and future perspectives. The Journal of Plant Protection Sciences, 2(1): 1-18
Mansour, A. and Al-Musa, A. (1992). Tomato yellow leaf curl virus: host range and virus relationships. Plant Pathology, 41: 122-125
Melzer, M.J., Ogata, D.Y., Fukuda, S.K., Shimabuku, R., Borth., W.B., Sether, D.M, Hu, J.S. (2009). Tomato yellow leaf curl. CTAHR PD-70 (http://www.ctahr. hawaii.edu/oc/freepubs/pdf/RES-078.pdf).
NHB (2013). Handbook of Indian Horticulture Database. NHB. Gurgaon, Haryana, India. http://nhb.gov.in
Oetting, R., and Yunis, H. (2004). Field guide to common insects, mites and diseases of greenhouse grown sweet peppers and tomatoes. Kfar Qari: Hakohav Press. pp 69
Reddy, B.A. (2006). Molecular characterization, epidemiology and management of tomato leaf curl virus (ToLCV) in Northern Karnatka. Ph.D thesis, Department of Plant pathology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad, India.
Sahu, D.K., Khare, C.P., Singh, H.K., and Thakur, M.P. (2013). Evaluation of newer fungicide for management of early blight of tomato in Chhattisgarh. The Bioscan, 8(4): 1255-1259
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