Influence of salicylic acid on biochemical parameters and antioxidant system in mashbean plants grown under salt stress conditions
Article Main
Abstract
Abiotic stress factors affect almost every aspect of physiology and biochemisrtry of a plant. The present study investigates the role of salicylic acid (SA) in inducing plant tolerance to salinity. The application of 0.5 mM and 1.0 mM SA to mashbean (Vigna mungo L.) plants provided protection against 30mM or 45mM NaCl stress through
elevated antioxidant system. The genotypes KUG 363, KUG 310, (salt sensitive), KUG 502 and KUG 529 (salt tolerant) along with UL 338 (as check) were subjected to salt stress. Relative leaf water content (61%) decreased under 45mM salt stress in salt tolerant genotype KUG 529 as compared to control (85%). Leaf water potential was also recorded at 50 DAS in salt tolerant genotype KUG 529 (-2.66 mpa) and in salt sensitive genotype KUG 363(-3.76 mpa) .All the genotypes showed higher accumulation of Reactive Oxygen Species under salt stress. A remarkable decrease was shown in antioxidant enzymes like catalase (179 micro mole/min/g FW) and ascorbate peroxidase (1617 n moles/min/g FW) in KUG 529 . The level of antioxidant system was enhanced catalase (184 micro mole/min/g FW) and ascorbate peroxidase (1853 n moles/min/g FW) in mashbean plants under NaCl stress following SA applications . Thus SA helped in conferring stress tolerance to mashbean plants through enhanced antioxidant system. However, tolerant genotypes responded better than sensitive ones and lower concentration of SA (0.5mM) was more effective.
Article Details
Article Details
Keywords
Antioxidant enzymes, Mashbean, Salicylic acid, Salt stress
References
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Anonymous. (2012). Project coordinaters report. All India Coordinated Research Project on MULLa RP. Pp:17
Bajji, M., Kinet, J. M and Lutts, S. (2001). The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant growth regulation, 1: 1-10
Barz, H.D., and Weatherley, P.E., (1962). A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15:413-428
Breusegem ,F., V., Vranova, E., Datt, J. F. and Inze, D. (2001) The role of active oxygen species in plant signal transduction. Plant Science, 161: 405-414
Chance ,M. and Maehly, A. C. (1995). Assay of catalases and peroxidases. Methods in Enzymology, 2: 764-817
Cheng,W.,J., Yan, L.,S. and Dong, H.,C. (2012). Changes in photosynthetic rate, water potential, and proline content in kenaf seedlings under salt stress. Canadian Journal of Plant Science, 92(2): 311-319.
Cho ,U. and Park, J. (2000). Mercury induced oxidative stress in tomato seedlings. Plant science, 156(1):1-9
Delavari, P. M., Baghizadeh, A., Enteshari, S. H., Kalantari, K. M., Yazdanpanah, A. and Mousavi, E. A. (2010). The effects of salicylic acid on some of biochemical and morphological characteristic of Ocimum Basilicucm under salinity stress. Australian Journal of Basic and Applied Sciences, 4 (10): 4832-4845
Dhindsa, R. S. and Motowe, W. (1981) Drought tolerance in two mosses: correlation with enzymatic defense against lipid peroxidation. Journal of Experimental Botany, 32: 79-91
El-Bassiouny, Hala M.S., and Bekheta, M. A. (2005). Effect of salt stress on relative water content, lipid peroxidation, polyamines, amino acids and ethylene of two wheat cultivars. International journal of agriculture & biology, 7 (3): 363-368
Erdal, S., Ayd?n, M., Genisel, M., Tasp?nar, M.S., Dumlupi-nar, R., Kaya, O. and Gorcek, Z. (2011). Effects of salicylic acid on wheat salt sensitivity. African Journal of Biotechnology, 10 (30): 5713-5718
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Ghai, N., Kaur, R., Pahwa, K., Kaur, J. and Singh, I. (2014). Ameliorative effects of salicylic acid on some physiological and biochemical attributes in mashbean (Vigna mungo L. hepper) under NaCl stress. Interna-tional journal of advanced research, 2(3): 942-955
Gunes, A., Inal, A., Alpaslan, M., Eraslan, F., Bagei, E. G. and Cicek, N. (2007). Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. Journal of Plant Physiology, 164: 728-736
Heath, R. L. and Packer, L. (1969). Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198
Hussein, M. M., Balbaa, L. K. and Gaballah, M. S. (2007) Salicylic acid and salinity effects on growth of maize plants. Research Journal of Agriculture and Biological sciences, 3: 321-328
Joseph, B. and Jini, D. (2011). Development of salt stress tolerant plants by gene manipulation of antioxidant enzymes. Asian Journal of Agricultural Research, 5(1): 17-27
Karlidag, H., Yildirim, E. and Turan, N. (2009). Salicylic acid ameliorate the adverse effect of salt stress on strawberry. Science Agriculture (Piracicaba,Braz), 66(2): 180-187
Kaur, H. (2009) Physiological changes associated with salinity stress in mashbean (Vigna mungo L. Hepper). M.sc. Thesis, Punjab agricultural University, Ludhiana, India.
Kaur, R. (2011) Ameliorative effects of salicylic acid on physiological changes associated with salinity stress in mashbean (Vigna mungo L. Hepper). M.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.
Khan, N. A., Syeed, S., Masood, A., Nazar, R. and Iqbal, N. (2010). Application of salicylic acid increases contents of nutrients and antioxidative metabolism in mungbean and alleviates adverse effects of salinity stress. Internationl journal of plant biology, 1: 1-8
Mohammadreza, S. , Amin, B. , Forogh, A., Hossin, M. and Sorayya, S. (2012). Response of Brassica napus L grains to the interactive effect of salinity and salicylic acid. Journal of Stress Physiology and Biochemistry, 8 (2): 159-166.
Molassiotis, A. N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G. and Therios, I. (2006). Antioxidant and anatomical responses in shoot culture of the apple rootstock. MM 106 treated with NaCl, KCl, mannitiol or sorbitol. Biologia Plantarum, 50(1): 61-68
Nakano, Y. and Asado, K. (1987). Purification of ascorbate peroxidase from spinach chloroplasts: its activation in ascorbate - depleted medium and reactivation by monodehydro-ascorbate radical. Plant Cell Physiology, 28: 131-40
Parida, A. K. and Das, A. B. (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60: 324-349
Pooja and Sharma, K. D. (2010) Salicylic acid induced amelioration in growth, biochemical metabolites and yield of mungbean (Vigna radiata L.) under salinity stress. Indian Journal of Plant Physiology, 15 (3): 219-225
Purcarea, C. and Cachita-Cosma, D. (2010) Studies regard-ing the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress. 20(1): 63-68 Seria Stiintele Vietii Vasile Goldis University, Romania.
Sevengor, S., Fikret, Y., Kusvuran, S. and Ellialtioglu, S. (2011). The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. African Journal of Agricultural Research, 6 (21): 4920-4924.
Shakya, S. K. and Singh, J. P. (2010). New drainage technologies for salt-affected waterlogged areas of south-west Punjab, India. Current Science, 99(2): 204-212
Silva, C., Martinez, V. and Carvajal, M. (2008). Osmotic versus toxic effects of NaCl on pepper plants. Biologia Plantarum, 52(1): 72-79.
Simaei,1. M., Nejad, R. A. K., Saadatmand, S., Bernard, F. and Fahimi, H. (2011). Effects of salicylic acid and nitric oxide on antioxidant capacity and proline accumulation in Glycine max L. treated with NaCl salinity. African Journal of Agricultural Research, 6(16): 3775-3782
Vellikova, V., Yordanov, I. and Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain treated bean plants. Plant Science, 151: 59-66.
Andre, N., Jose, T.P., Joaquim, E.F. and Carlo- Eduardo, B. D. A. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environmental and Experimental Botany, 56: 87-94.
Anonymous (2009). Anti-waterlogging project, department of irrigation and drainage, Punjab Government.
Anonymous. (2012). Project coordinaters report. All India Coordinated Research Project on MULLa RP. Pp:17
Bajji, M., Kinet, J. M and Lutts, S. (2001). The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant growth regulation, 1: 1-10
Barz, H.D., and Weatherley, P.E., (1962). A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15:413-428
Breusegem ,F., V., Vranova, E., Datt, J. F. and Inze, D. (2001) The role of active oxygen species in plant signal transduction. Plant Science, 161: 405-414
Chance ,M. and Maehly, A. C. (1995). Assay of catalases and peroxidases. Methods in Enzymology, 2: 764-817
Cheng,W.,J., Yan, L.,S. and Dong, H.,C. (2012). Changes in photosynthetic rate, water potential, and proline content in kenaf seedlings under salt stress. Canadian Journal of Plant Science, 92(2): 311-319.
Cho ,U. and Park, J. (2000). Mercury induced oxidative stress in tomato seedlings. Plant science, 156(1):1-9
Delavari, P. M., Baghizadeh, A., Enteshari, S. H., Kalantari, K. M., Yazdanpanah, A. and Mousavi, E. A. (2010). The effects of salicylic acid on some of biochemical and morphological characteristic of Ocimum Basilicucm under salinity stress. Australian Journal of Basic and Applied Sciences, 4 (10): 4832-4845
Dhindsa, R. S. and Motowe, W. (1981) Drought tolerance in two mosses: correlation with enzymatic defense against lipid peroxidation. Journal of Experimental Botany, 32: 79-91
El-Bassiouny, Hala M.S., and Bekheta, M. A. (2005). Effect of salt stress on relative water content, lipid peroxidation, polyamines, amino acids and ethylene of two wheat cultivars. International journal of agriculture & biology, 7 (3): 363-368
Erdal, S., Ayd?n, M., Genisel, M., Tasp?nar, M.S., Dumlupi-nar, R., Kaya, O. and Gorcek, Z. (2011). Effects of salicylic acid on wheat salt sensitivity. African Journal of Biotechnology, 10 (30): 5713-5718
Fahramand, M., Mahmoody, M., Keykha, A., Noori, M. and Rigi, K. (2014) Influence of abiotic stress on proline, photosynthetic enzymes and growth. International Research Journal Applied and Basic Science, 8(3): 257-265
Ghai, N., Kaur, H., Kaur, J., Sandhu, J. S. and Singh, I. (2010). Influence of salinity on growth and yield attributes in mashbean (Vigna mungo L.) genotypes. Journal Research of Punjab agricultural University, 47: 58-66
Ghai, N., Kaur, R., Pahwa, K., Kaur, J. and Singh, I. (2014). Ameliorative effects of salicylic acid on some physiological and biochemical attributes in mashbean (Vigna mungo L. hepper) under NaCl stress. Interna-tional journal of advanced research, 2(3): 942-955
Gunes, A., Inal, A., Alpaslan, M., Eraslan, F., Bagei, E. G. and Cicek, N. (2007). Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. Journal of Plant Physiology, 164: 728-736
Heath, R. L. and Packer, L. (1969). Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198
Hussein, M. M., Balbaa, L. K. and Gaballah, M. S. (2007) Salicylic acid and salinity effects on growth of maize plants. Research Journal of Agriculture and Biological sciences, 3: 321-328
Joseph, B. and Jini, D. (2011). Development of salt stress tolerant plants by gene manipulation of antioxidant enzymes. Asian Journal of Agricultural Research, 5(1): 17-27
Karlidag, H., Yildirim, E. and Turan, N. (2009). Salicylic acid ameliorate the adverse effect of salt stress on strawberry. Science Agriculture (Piracicaba,Braz), 66(2): 180-187
Kaur, H. (2009) Physiological changes associated with salinity stress in mashbean (Vigna mungo L. Hepper). M.sc. Thesis, Punjab agricultural University, Ludhiana, India.
Kaur, R. (2011) Ameliorative effects of salicylic acid on physiological changes associated with salinity stress in mashbean (Vigna mungo L. Hepper). M.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.
Khan, N. A., Syeed, S., Masood, A., Nazar, R. and Iqbal, N. (2010). Application of salicylic acid increases contents of nutrients and antioxidative metabolism in mungbean and alleviates adverse effects of salinity stress. Internationl journal of plant biology, 1: 1-8
Mohammadreza, S. , Amin, B. , Forogh, A., Hossin, M. and Sorayya, S. (2012). Response of Brassica napus L grains to the interactive effect of salinity and salicylic acid. Journal of Stress Physiology and Biochemistry, 8 (2): 159-166.
Molassiotis, A. N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G. and Therios, I. (2006). Antioxidant and anatomical responses in shoot culture of the apple rootstock. MM 106 treated with NaCl, KCl, mannitiol or sorbitol. Biologia Plantarum, 50(1): 61-68
Nakano, Y. and Asado, K. (1987). Purification of ascorbate peroxidase from spinach chloroplasts: its activation in ascorbate - depleted medium and reactivation by monodehydro-ascorbate radical. Plant Cell Physiology, 28: 131-40
Parida, A. K. and Das, A. B. (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60: 324-349
Pooja and Sharma, K. D. (2010) Salicylic acid induced amelioration in growth, biochemical metabolites and yield of mungbean (Vigna radiata L.) under salinity stress. Indian Journal of Plant Physiology, 15 (3): 219-225
Purcarea, C. and Cachita-Cosma, D. (2010) Studies regard-ing the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress. 20(1): 63-68 Seria Stiintele Vietii Vasile Goldis University, Romania.
Sevengor, S., Fikret, Y., Kusvuran, S. and Ellialtioglu, S. (2011). The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. African Journal of Agricultural Research, 6 (21): 4920-4924.
Shakya, S. K. and Singh, J. P. (2010). New drainage technologies for salt-affected waterlogged areas of south-west Punjab, India. Current Science, 99(2): 204-212
Silva, C., Martinez, V. and Carvajal, M. (2008). Osmotic versus toxic effects of NaCl on pepper plants. Biologia Plantarum, 52(1): 72-79.
Simaei,1. M., Nejad, R. A. K., Saadatmand, S., Bernard, F. and Fahimi, H. (2011). Effects of salicylic acid and nitric oxide on antioxidant capacity and proline accumulation in Glycine max L. treated with NaCl salinity. African Journal of Agricultural Research, 6(16): 3775-3782
Vellikova, V., Yordanov, I. and Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain treated bean plants. Plant Science, 151: 59-66.
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Influence of salicylic acid on biochemical parameters and antioxidant system in mashbean plants grown under salt stress conditions. (2016). Journal of Applied and Natural Science, 8(4), 1786-1792. https://doi.org/10.31018/jans.v8i4.1041
