Characterization and evaluation of heavy metal tolerance of bacterial species from soil of waste area near Riyan steel rolling mills, Muzaffarnagar, India
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Abstract
The present study observed a variety of microorganisms from the soil of the waste area near Riyan Steel Rolling Mills , Muzaffarnagar. Among the microorganisms, 10 isolates of Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus showed great potential against detrimental heavy metals like Hg, Pb, Zn. Out of these 10 isolates, only 2 isolates of Bacillus subtilis (N1A2, N1P2), one isolates of P. aeruginosa N1A4 and one isolates of S. aureus A1N3 showed tremendous tolerance against various heavy metals. Bacillus subtilis N1A2 showed
15x109 cfu/ml in 100?g/ml of HgCl2 whereas B. subtilis N1P2 showed 15x109 cfu/ml in 400?g/ml of zinc acetatate. Similarily Pseudomonas aeruginosa N1A4 showed 11x109 cfu/ml in 150?g/ml of lead acetate. Staphylococcus aureus A1N3 showed great resistance towards 450?g/ml of zinc sulphate and maintain their growth up to 7x109 cfu/ml. strains were identified as Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus on the basis of their morphological, physiological and biochemical and compared with their standard MTCC strains.
Article Details
Article Details
Heavy metals, CFU, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus
Deeb, B. E. (2009). Plasmid mediated tolerance and removal of heavy metals by Enterobacter sp. American Journal of Biochemistry and Biotechnology, 5 (1): 47-53
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., and Williams, S.T. (1994). In, Bergey’s manual of Determinative Bacteriology. Williams and Wilkins Press, Baltimore, U.S.A.
Hughes, M.N. and Poole, R.K. (1991). Metal speciation and microbial growth, the hard and soft facts. Journal of General Microbiology, 137: 725-734.
Nriagu, J.O. and Pacyna, J.M. (1998). Quantitative assessment of worldwide contamination of air, water and soil by trace metal, Nature 333: 134-139. Nies, D.H. (1999). Microbial heavy metal resistance. Applied Microbiology and Biotechnology, 51: 730-750.
Onalaja, A.O., Claudio L. (2000). Genetic susceptibility to lead poisoning. Environ Health Perspec., 1:23-38.
Pahan, K., Ghosh, D.K., Chaudhuri, J., Gachhui, R., Ray, S. and Mandal, A. (1995). Mercury detoxifying enzymes within endospores of a broad-spectrum mercury resistant bacillus pasteurii strain DR2. Journal of Bioscience, 20: 83–88.
Rajbanshi, A. (2008). Study on heavy hetal resistant bacteria in Guheswori sewage treatment plant. Our Nature, 6: 52-57.
Roane, T. M. and Kellogg, S. T. (1995). Characterization of bacterial communities in heavy metal contaminated soils. Canadian journal of Microbiology, 42: 593- 603.
Silver, S. and Misra, Ô. Ê. (1988). Plasmid mediated heavy metal resistances; Annual Review of Microbiology, 42: 717-743.
Sobolev D. and Begonia M. F. T. (2008). Effects of heavy metal contamination upon soil microbes: Lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers. Int. J. Environ. Res. Public Health, 5 (5): 450-456.
USPHS (1997). Toxicological profile on CD-ROM. Agency for Toxic Substances and Disease Registry.
Wu, J. J., Schuch, R. and Piggot, Ñ. J. (1992). Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DDcarboxy-
peptidase; Journal of Bacteriology, 174: 4885-4892.
Wuertz, S. and Mergeay M. (1997). The impact of heavy metals on soil microbial communities and their activities. In: Van Elsas JD, Trevor’s JT, Wellington EMH. (Eds). Modern Soil Microbiology Marcel Dekker, New York, pp. 607 – 639.
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