Assessment of genetic diversity in Indian Barnyard millet (Echinochloa spp. complex) using morphological and molecular markers
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Abstract
In the present study, morphological and molecular markers (RAPD primers) were used to analyze the genetic diversity and genetic relationships among 21 accessions of Echinochloa spp. complex comprising the wild and cultivated species collected from Melghat and adjoining regions of Vidarbha, Maharashtra. The availability of diverse genetic resources is a prerequisite for genetic improvement of any crop including barnyard millet. A high degree of molecular diversity among the landraces was detected. Among the 21 genotypes, two major groups (A and B) were formed, at 67.28 % similarity, which clearly encompasses 15 accessions of E. frumentacea and 6 accessions of E. colona. Higher similarity was observed in accessions of E. frumentacea. The accessions IC 597322 and IC 597323 also IC 597302 and IC 597304 showed more than 94% similarity among themselves. The classification of genetic diversity has enabled clear-cut grouping of barnyard millet accessions into two morphological races (E. frumentacea and E. colona).
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Barnyard millet, Genetic diversity, Genetic relationship, RAPD marker
Doyle, J. J., and Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin, 19:11-15
Gupta, A., Mahajan, V., Mukesh Kumar and Gupta, H. S. (2009). Biodiversity in the barnyard millet (Echinochloa frumentacea Link, Poaceae) germplasm in India. Genetic Resources Crop Evolution, 56:883–889
Holm L.G., Plucknett D.L., Pancho J.V., and Herberger J.P. (1977). The World's Worst Weeds. Distribution and Biology. (Universiti Press,Honolulu, Hawaii).
Linacero, R.J., Rueda and vazquez A.M. (1998). Quantifying of DNA. Karp, A., P.G. Isaac, and D.S. Ingram (eds.) Molecular tools for screening biodiversity: plants and animals. Chapman and Hall. London, Weinheim, New york, Tokyo, Melbourne, Mmadras. pp. 18-21.
Michael, P.W. (1994). Distribution and taxonomy of Echinochloa – A world view with a key to the species occurring in China. Proceedings of the 5th Weed Science Conference of China, pp. 161-6.
Roy, S., Simon, J. P., and Lapointe, F. J. (2000). Determination of the origin of the cold adapted population of barnyard grass (Echinochloa crus-galli) in eastern North America: A total evidence approach using RAPD DNA and DNA sequences. Canadian Journal of Botany, 78: 1505-1513.
Sambrook, J., Frilsch, E. F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd Edn. Cold spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Tasrif, A., Juraimi, A. S., Kadir, J., Napis, S. and Sastroutomo, S. S. (2003). Morphological and molecular variation among Indonesian ecotypes of barnyardgrass (Echinochloa crus-galli var. Crusgalli L. Beauv). In Proceeding of the 15th Conference of Indonesian Weed Science Society.
Ugare, R., Chinmad, B., Naik, R., Bharati, P. and Itagi, S. (2011). Glycaemic index and significance of barnyard millet (Echinochloa frumentacea ) in type II diabetes. Journal of Food Science and Technology, DOI:10.1007/s13197-011-0516-8.
Watson, L. and Dallwitz, M.J. (1992). ‘The grass genera of the world’. (CAB International, Oxford, UK).
Yabuno, T. (1962). Cytotaxonomic studies on the two cultivated species and the wild relatives in the genus Echinochloa. Cytologia, 27:296-305.
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