Optimization of culture conditions for high frequency in vitro shoot multiplication in sugarcane (Saccharum officinarum L.)
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Abstract
Present study deals with the optimization of various culture conditions for initiating high frequency in vitro shoot multiplication in two early maturing high yielding sugarcane genotypes namely Co98014 & Co89003. On the behalf of the findings of this study, it was concluded that the temperature, photoperiod and culture media pH affected the frequency of in vitro shoot multiplication in both sugarcane genotypes at a significant level. In both genotypes high frequency shoot multiplication was recorded at growth room temperature 25ºC, 16h/8h light/dark photoperiod and culture media pH 6.0. Genotype Co89003 exhibited highest shoot regeneration and multiplication under various culture conditions. The present study suggests the necessity of investigation of these culture conditions separately upon individual sugarcane genotypes prior to develop efficient in vitro plant regeneration protocol for commercial purposes.
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Keywords
Culture environment, Shoots regeneration, Sugarcane, Tissue culture
References
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Arencibia, A.E., Carmona, P., Tellez, M.T., Chan, S.M., Yu, L., Trujillo, and Oramas, P. (1998). An efficient protocol for sugarcane (Saccharum spp.) transformation mediated by Agrobacterium tumefaciens. Transgenic Research, 7: 213-222.
Commodity Research Bureau. (2015). The 2015 CRB commodity yearbook. Chicago, IL: Commodity Research Bureau.
FAO (2013). www.fao.org
FAOSTAT. (2015). Statistical Division: Production domain- Crops. Updated on November 24, 2015; Retrieved on December 18, 2015
Fonnesbech, A. and Fonnesbech, M. (1980). In Vitro propagation of Monstera deliciosa. Horticultural Science, 15: 740-741.
Geetha, S., Padmanabhan, W., Manuel, W.W. and Ayyamperumal, A. (2000). In Vitro production of sugarcane plants. Sugar Tech, 2(3): 47-48.
Hendre, R.R., Mascarenhas, A.F., Nadgir, A.L., Pathak M. and Jagannathan, V. (1975). Growth of sugarcane mosaic virus-free sugarcane plants from apical meristems. Indian Phytopathology, 28: 175-178.
Jain, A., Inderjeet., Dhawan, A.K. and Dendsay, J.P.S. (1997). An efficient protocol for micropropagation in sugarcane. In: Proc. Natl. Sem. on Sucrose Synthesis and Recovery in Sugarcane: Issuses and Dimensions. Oct. 8-9, (1997), Karnal (Haryana). pp. 179-182.
Jalaja, N.C., Neelamathi, D. and Sreenivasan, T.V. (2008). Micropropagation for quality seed production in sugarcane. In: Manual on micropropagation for quality seed production in sugarcane in Asia and the Pacific. Food and Agriculture Organization of the United Nations (FAO), Rome. pp. 1-30.
Jo, E.A., Tiwari, R.K., Hahn, E.J. and Paek K.Y. (2008). Effect of photoperiod and light intensity on in vitro propogation of Alocasia amazonica. Plant Biotechnology Reports, 2: 207-212.
Kozai, T., Watanabe, K. and Jeong, B.R. (1995). Stem elongation and growth of potato (Solanum tuberosum L.) in vitro in response to photosynthetic photon flux, photoperiod and difference in photoperiod and dark period temperature. Scientia Horticulturae, 64: 1-9.
Lal, M., Singh, R.K., Srivastava, S., Singh, N., Singh, S.P. and Sharma, M.L. (2008). RAPD worker used analysis of micropropagated plantlets of Sugarcane for early evaluation of genetic fidelity. Sugar Tech, 10: 99-103.
Lal, N. (2003). High frequency of plant regeneration from sugarcane callus. Sugar Tech, 5(1&2): 89-91.
Mishra, S. (2011). Effect of Temperature, Photoperiod and pH on in vitro shoot multiplication of sugarcane. Vegetos, 24(1): 50-53.
Morini, S., Sciutti, R., Muleo, R. and Fortuma, P. (1991). Growth patterns of “in vitro” cultured shoot tips as influenced by different light - dark regimes. Acta Horticulturae, 289: 137-138.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays for tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Ramanand and Lal, M. (2004). An efficient protocol for in vitro micropropagation of sugarcane. Sugar Tech, 6 (1&2): 85-87.
Sengar, K., Sengar, R.S. and Garg, S.K. (2011). The effect of in vitro environmental conditions on some sugarcane varieties for micropropagation. African Journal of Biotechnology, 10(75): 17122-17126.
Sharma, M.K., Sengar, R.S., Chand, P., Singh, R., Kumar, P., Gupta, S. and Yadav, M.K. (2015). Effects of temperature, photoperiod and pH of culture media on in vitro shoot multiplication in sugarcane genotypes. Progressive Agriculture, 15(1): 104-108.
Singh, A. (2005). Physiological and biochemical basis of in vitro morphogenesis in sugarcane hybrids. Thesis submitted to CSM University, Kanpur, UP, India.
Tapingkae, T. and Tajii, A. (2000). Light quality and quantity: their effects on in vitro growth and development of two Australian plant species. Acta Horticulturae, 541: 281-288.
Tisserat, B. (1981). Date Palm tissue culture, U.S. Department of Agriculture Research Service, Oakland, California.
Wagih, M.E., Ala, A. and Musa, Y. (2004). Regeneration and evaluation of sugarcane somaclonal variants for drought tolerance. Sugar Tech, 6(1&2): 35-40.
Arencibia, A.E., Carmona, P., Tellez, M.T., Chan, S.M., Yu, L., Trujillo, and Oramas, P. (1998). An efficient protocol for sugarcane (Saccharum spp.) transformation mediated by Agrobacterium tumefaciens. Transgenic Research, 7: 213-222.
Commodity Research Bureau. (2015). The 2015 CRB commodity yearbook. Chicago, IL: Commodity Research Bureau.
FAO (2013). www.fao.org
FAOSTAT. (2015). Statistical Division: Production domain- Crops. Updated on November 24, 2015; Retrieved on December 18, 2015
Fonnesbech, A. and Fonnesbech, M. (1980). In Vitro propagation of Monstera deliciosa. Horticultural Science, 15: 740-741.
Geetha, S., Padmanabhan, W., Manuel, W.W. and Ayyamperumal, A. (2000). In Vitro production of sugarcane plants. Sugar Tech, 2(3): 47-48.
Hendre, R.R., Mascarenhas, A.F., Nadgir, A.L., Pathak M. and Jagannathan, V. (1975). Growth of sugarcane mosaic virus-free sugarcane plants from apical meristems. Indian Phytopathology, 28: 175-178.
Jain, A., Inderjeet., Dhawan, A.K. and Dendsay, J.P.S. (1997). An efficient protocol for micropropagation in sugarcane. In: Proc. Natl. Sem. on Sucrose Synthesis and Recovery in Sugarcane: Issuses and Dimensions. Oct. 8-9, (1997), Karnal (Haryana). pp. 179-182.
Jalaja, N.C., Neelamathi, D. and Sreenivasan, T.V. (2008). Micropropagation for quality seed production in sugarcane. In: Manual on micropropagation for quality seed production in sugarcane in Asia and the Pacific. Food and Agriculture Organization of the United Nations (FAO), Rome. pp. 1-30.
Jo, E.A., Tiwari, R.K., Hahn, E.J. and Paek K.Y. (2008). Effect of photoperiod and light intensity on in vitro propogation of Alocasia amazonica. Plant Biotechnology Reports, 2: 207-212.
Kozai, T., Watanabe, K. and Jeong, B.R. (1995). Stem elongation and growth of potato (Solanum tuberosum L.) in vitro in response to photosynthetic photon flux, photoperiod and difference in photoperiod and dark period temperature. Scientia Horticulturae, 64: 1-9.
Lal, M., Singh, R.K., Srivastava, S., Singh, N., Singh, S.P. and Sharma, M.L. (2008). RAPD worker used analysis of micropropagated plantlets of Sugarcane for early evaluation of genetic fidelity. Sugar Tech, 10: 99-103.
Lal, N. (2003). High frequency of plant regeneration from sugarcane callus. Sugar Tech, 5(1&2): 89-91.
Mishra, S. (2011). Effect of Temperature, Photoperiod and pH on in vitro shoot multiplication of sugarcane. Vegetos, 24(1): 50-53.
Morini, S., Sciutti, R., Muleo, R. and Fortuma, P. (1991). Growth patterns of “in vitro” cultured shoot tips as influenced by different light - dark regimes. Acta Horticulturae, 289: 137-138.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays for tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Ramanand and Lal, M. (2004). An efficient protocol for in vitro micropropagation of sugarcane. Sugar Tech, 6 (1&2): 85-87.
Sengar, K., Sengar, R.S. and Garg, S.K. (2011). The effect of in vitro environmental conditions on some sugarcane varieties for micropropagation. African Journal of Biotechnology, 10(75): 17122-17126.
Sharma, M.K., Sengar, R.S., Chand, P., Singh, R., Kumar, P., Gupta, S. and Yadav, M.K. (2015). Effects of temperature, photoperiod and pH of culture media on in vitro shoot multiplication in sugarcane genotypes. Progressive Agriculture, 15(1): 104-108.
Singh, A. (2005). Physiological and biochemical basis of in vitro morphogenesis in sugarcane hybrids. Thesis submitted to CSM University, Kanpur, UP, India.
Tapingkae, T. and Tajii, A. (2000). Light quality and quantity: their effects on in vitro growth and development of two Australian plant species. Acta Horticulturae, 541: 281-288.
Tisserat, B. (1981). Date Palm tissue culture, U.S. Department of Agriculture Research Service, Oakland, California.
Wagih, M.E., Ala, A. and Musa, Y. (2004). Regeneration and evaluation of sugarcane somaclonal variants for drought tolerance. Sugar Tech, 6(1&2): 35-40.
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How to Cite
Optimization of culture conditions for high frequency in vitro shoot multiplication in sugarcane (Saccharum officinarum L.). (2016). Journal of Applied and Natural Science, 8(3), 1565-1569. https://doi.org/10.31018/jans.v8i3.1001
