Effect of growth regulators on micropropagation of Rauvolfia serpentina (L.) Benth

Abstract

An efficient protocol for micropropagation through in vitro culture of Rauvolfia serpentina was standardized. Out of different combination of phytohormone tested, MS media supplemented with 0.5 mg L-1Indole Acetic Acid + 0.5 mg L Nephthalene acetic acid was found to be finest for mean callus induction (62.66%) as well as callus mediated shoot regeneration with mean percentage response (56) and number of shoot per culture (5). In direct shoot regeneration, best growth of axillary shoots was obtained on MS media supplemented with 0.5 mg L-1 Indole Acetic Acid + 0.5 mg L-1 Benzyl Amino Purine with maximum mean percentage response(77.33) and number of shoots per culture (9.0) ,however the best shoot elongation of shoot was found on MS media supplemented with 3.0 mg L-1 IAA plus 3.0 mg L-1BAP with 6.50(mean) . Higher induction of root (88%) with mean number of root per culture (12) was observed in MS medium supplemented with Indole Butyric Acid (3.0 mg L-1). The rooted plantlets were successfully established in the field. The protocol was optimized by manipulations of different PGRs for enhanced multiplication. Protocol explained in this research paper provides a rapid plant regeneration system which could be used for production of large number of true to the type, uniform, disease free, elite, plantlets right through the year, which will make things easier for large scale cultivation of this endangered important medicinal plant.