In-vitro analysis of potential antibacterial activity of three medicinal plants
##plugins.themes.bootstrap3.article.main##
Abstract
The present study was aimed to examine and compare the antibacterial activity of hot methanolic extract of medicinal plants viz. Portulaca oleracea (purslane), Syzygium cumini (L.) (jamun), Psidium guajava (L.) (guava). Antibacterial activity was carried by using agar well diffusion method, against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Results indicated that all the three plant extracts possess antibacterial property against Gram-positive bacteria and no activity was found against Gram-negative bacteria. Moderate zone of inhibition against Staphylococcus aureus and Bacillus subtilis was exhibited by S. cumini (L.) (11mm and 12mm) and P. guajava (L.) (10mm and 11mm) and weak zone of inhibition was exhibited by P. oleracea (5 mm and 6mm). In conclusion, S. cumini (L.) and P. guajava (L.) possess better
capabilities of being a good candidate in search for natural antibacterial agent against infections and diseases causing Gram-positive bacteria as compared to P. oleracea.
##plugins.themes.bootstrap3.article.details##
##plugins.themes.bootstrap3.article.details##
Agar well diffusion method, Antibacterial activity, Gram-negative bacteria, Gram-positive, Medicinal plants
Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., and Yadav, A.(2013). Antimicrobial activities of leaf ex-tracts of guava (Psidium guajava L.) on two Gram-Negative and Two Gram-Positive Bacteria. Interna-tional Journal of Microbiology.2013:1-7
Chaudhary, B. and Mukhopadhyay, K.(2012). Syzygium cumini (L.) Skeels: A Potential Source of Nutraceuti-cals. International Journal of Pharmacy and Biological Sciences. 2(1): 46-53.
Choudhary, S., Sharan, L. and Sinha, M.P.(2012). Phyto-chemical and Antimicrobial Screening of psidium Gua-java L. Leaf Extracts against Clinically Important Gas-trointestinal Pathogens, J.Nat. Prod. Plant Resour. 2 (4):524-529.
Dey, S.K., Banerjee, D., Chattapadhyay, S. and Karmakar, K.B. (2010). Antimicrobial activities of some medicinal plants of West Bengal. International journal of Pharma and Bio Sciences.1(3):1-10.
Dixit, P., Tripathi, S., Verma, K.N. 2013. A Brief Study On Marigold (Targets Species): A Review. International Research Jaurnal Of Pharmacy. 4(1)43-48.
Donehower, R.C. and Rowinsky, E.R. (1993). Cancer Treat Rev 1993;19C:63 Egga E.S., Adeyanju O., Agyens O.E. 2014. Preliminary Phytochemical, Antibacterial and Proximate Analysis of Tender Leaves of Psidium gua-java L. in Jos, Plateau State, Nigeria. Asian Review of Environmental and Earth Sciences. 1(2):35-38
Goncalves, F.A., Andrade, M., Bezerra, J.N.S et al. (2008). Antibacterial activity of guava, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from seabob shrimp, Xiphopenaeus Kroyeri (Heller),” Revista do Instituto de Medicina Tropical de Sao Paulo. 50(1):11-15
Handa, D. and Sharma, K. (2014). A Review: Plant Extracts a New Source of Antimicrobial Potential. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 5(3): 597-628.
Hostettmann, K., Marston, A., Maillard, M., Hamburger, M.(1995). Phytochemistry of plants used in traditional medicine. Claredon Press, Oxford.
Mohamed, A.A., Ali S.L.,EL-Baz, F.K. 2013. Antioxidant and Antibacterial Activities of Crude Extracts and Essential oils of Syzygium cumini Leaves. PLoS One. 8(4):e60269.doi:10.1371/journal.pone.0060269
Mujeeb, F., Bajpai, P., and Pathak, N.(2014). Phytochemcial Evaluation, Antibacterial Activity, and Determination of Bioactive Components from leabes of Aegle Marmelos. BioMed Research International.2014:1-11
Ncube, N.S., Afolayan, A.J., and Okoh, A.I. (2008). Assess-ment of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afri-can Journal of Biotechnology. 7(12): 1797-1806
Sanches, N.R., Cortez, D.A.G., Schiavini, M.S., Nakamura, C.V., and Filho, B.P.D.(2005). An evaluation of anti-bacterial activities of Psidium guajava (L.).” Brazilian Archives of Biology and Technology. 48(3):429-436
Shafi, P.M., Rosamma, M.K., Jamil, K., Reddy, P.S. (2002).Antibacterial activity of Syzygium cumini and Syzygium travancoricum leaf essential oils. Fitoterapia 73 (5): 414–416. doi: 10.1016/s0367-326x(02)00131-4
Shyamala, S.G., Vasantha, K. (2010). Phytochemical screen-ing and antibacterial activity of Syzygium cumini (l.) (Myrtaceae) leaves extracts. Int J Pharm Technol Res. 2(2):1569-1573
Sumitra C. and Kaneria M. 2011. Indian nutraceutical plant leaves as potential source of natural antimicrobial agents. Retrieved from http://www.formatex.info/microbiology3/book/1251-1259.pdf
Vieira, R.H.S.D.F., Rodrigues, D.D.P., Goncaves, F.A., Menezes, F.G.R., Aragao, J.S. and Sousa, O.V. (2001). Microbial effect of medicinal plant extracts to acne-developing organisms. The American Journal of Chi-nese Medicine. 33(2):197-204.
World Health Organization. Antimicrobial resistance: no action today, no cure tomorrow. http://www.who.int/world-health-day/2011/en/index.html (19 April 2011, date last accessed).
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
