##plugins.themes.bootstrap3.article.main##

Vivekanand P. Rao Sanjay Singh R. Chaudhary M. K. Sharma R.S. Sengar Uma M. Singh Vinay Sharma

Abstract

In the present study, 14 sugarcane (Saccharum spp. hybrid) genotypes were used for genomic diversity analysis based on nineteen inter simple sequence repeat (ISSR). These nineteen sets of ISSR markers generated a total of 164 discernible and reproducible bands including 109 polymorphic and 55 monomorphic bands. The unweighted pair group method with arithmetic average (UPGMA) analysis revealed three distinct clusters: I, II and III within the 14 genotypes. The polymorphic information content (PIC) value per locus ranged from 0.14 (UBC811) to 0.53 (ISSR1) locus with an average of 0.42 for all loci. The range of genetic distance or coefficient of similarity among sugarcane genotypes varied 0.14 - 0.78. The analysis of these similarities matrix revealed that greater similarity between CoS03234 and CoSe1424 (0.78), and lowest similarity between CoS03234 and Co0118 (0.14). The knowledge gained in this study would be useful to future breeding programs for increasing genetic diversity of sugarcane varieties and cultivars to meet the increasing demand of sugarcane cultivation for sugar and bio energy uses.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Genetic diversity, Inter simple sequence repeat (ISSR) marker, Unweighted pair group method with arithmetic average (UPGMA)

References
Al-Turki,T.A. and Basahi M. A. (2015). Assessment of ISSR based molecular genetic diversity of Hassawi rice in Saudi Arabia. Saudi Journal of Biological Sciences. 22 : 591-592.
Costa, M.L., Amorim, L.L.B., Onofre, A.V.C., Melo, L.J.O.T., Oliveira, M.B.M., Carvalho, R. and Iseppon, A.M.B. (2011). Assessment of Genetic Diversity in Contrasting Sugarcane Varieties Using Inter-Simple Sequence Repeat (ISSR) Markers. American Journal of Plant Sciences. 2 : 425-432.
Devarumath, R.M., Kalwade, S.B., Kawar, P.G. and Sushir, K.V. (2012). Assessment of Genetic Diversity in Sugarcane Germplasm Using ISSR and SSR Markers. Sugar Tech. 14 : 334–344.
FAOSTAT. (2015). Statistical Division: Production domain- Crops. Updated August 24, 2015; Retrieved on December 21, 2015.
He, W.H., Xu, C.Y., He, L.F., Li, C.Z. and Wei, S.Q. (2011). Assessment of genetic diversity amongst sugarcane varieties from different regions using ISSR markers. Proceeding of 4th IAPSIT, Balancing Sugar and Energy Production in Developing Countries: Sustainable technology and Marketing Strategies, New Delhi, India. pp. 695-700.
Hoisington, D., Khairallah, M. and Gonzalez-de-Leon, D. (1994). Laboratory protocols: CIMMYT applied molecular genetics laboratory, 2nd ed. Mexico. CIMMYT.
Jaccard, P. (1908). Nouvelles recherches sur la distribution florale. Bul. Soc. Vaudoise. Sci. Nat. 44 : 223-270.
Kalwade, S. B., Devarumath, R. M., Kawar, P. G., Sushir, K. V. (2012). Genetic profiling of sugarcane genotypes using Inter Simple Sequence Repeat (ISSR) markers. Electronic Journal of Plant Breeding. 3 : 621-628.
Nei, M. and Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. 76 : 5269-5273.
Pandey, R. N., Singh, J., Rastogi, S. P., Sharma, M. L. and Singh, R. K. (2012). Early assessment of genetic fidelity in sugarcane (Saccharum officinarum) plantlets regenerated through direct organogenesis with ISSR and SSR markers. Australian Journal of Crop Science. 6 : 618-624.
Rao, V. P., Chaudhary, R., Singh, S., Sengar, R. S. and Sharma, V. (2014). Assessment of genetic diversity analysis in contrasting sugarcane varieties using Random Amplified Polymorphic DNA (RAPD) markers. African Journal of Biotechnology. 13 : 3736-3741.
Sadigova, S., Sadigov, H., Eshghi, R., Salayeva, S. and Ojaghi, J. (2014). Application of RAPD and ISSR markers to analyses molecular relationships in Azerbaijan wheat accessions (Triticum aestivum L.). Bulg. J. Agric. Sci. 20 : 87-95.
Singh, S., Gautam, R. K., Singh, R. K., Deshmukh, R. and Ojha, A. (2010). Molecular diversity in rice genotypes differing in physiological mechanism of salt tolerance through SSR and ISSR markers. IJABPT. 1 : 550-560.
Tabasum, S., Khan, F. A., Nawaz, S., Iqbal M. Z. and Saeed, A. (2010). DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA. Genetics and Molecular Research. 9 : 471-483
Zhang, H. Y., Li, F. S., Liu, X. Z., He, L. L., Yang, Q. H. and He, S. C. (2008). Analysis of genetic variation in Erianthus arundinacues by Inter simple sequence repeat markers. African Journal of Biotechnology. 7 : 3414-3418.
Citation Format
How to Cite
Rao, V. P., Singh, S., Chaudhary, R., Sharma, M. K., Sengar, R., Singh, U. M., & Sharma, V. (2016). Genetic variability in sugarcane (Saccharum spp. hybrid) genotypes through inter simple sequence repeats (ISSR) markers. Journal of Applied and Natural Science, 8(3), 1404–1409. https://doi.org/10.31018/jans.v8i3.973
More Citation Formats:
Section
Research Articles