Genetic variability in sugarcane (Saccharum spp. hybrid) genotypes through inter simple sequence repeats (ISSR) markers

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Published Sep 1, 2016
DOI: https://doi.org/10.31018/jans.v8i3.973

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Vivekanand P. Rao
Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut- 250 110 (U.P.), INDIA
Sanjay Singh
Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut- 250 110 (U.P.), INDIA
R. Chaudhary
Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut- 250 110 (U.P.), INDIA
M. K. Sharma
Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut- 250 110 (U.P.), INDIA
R.S. Sengar
Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut- 250 110 (U.P.), INDIA
Uma M. Singh
Department of Molecular Biology & Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145 (Uttarakhand), INDIA
Vinay Sharma
Department of Bioscience and Biotechnology, Banasthali University Banasthali- 304 022 (Rajasthan), INDIA

Abstract

In the present study, 14 sugarcane (Saccharum spp. hybrid) genotypes were used for genomic diversity analysis based on nineteen inter simple sequence repeat (ISSR). These nineteen sets of ISSR markers generated a total of 164 discernible and reproducible bands including 109 polymorphic and 55 monomorphic bands. The unweighted pair group method with arithmetic average (UPGMA) analysis revealed three distinct clusters: I, II and III within the 14 genotypes. The polymorphic information content (PIC) value per locus ranged from 0.14 (UBC811) to 0.53 (ISSR1) locus with an average of 0.42 for all loci. The range of genetic distance or coefficient of similarity among sugarcane genotypes varied 0.14 - 0.78. The analysis of these similarities matrix revealed that greater similarity between CoS03234 and CoSe1424 (0.78), and lowest similarity between CoS03234 and Co0118 (0.14). The knowledge gained in this study would be useful to future breeding programs for increasing genetic diversity of sugarcane varieties and cultivars to meet the increasing demand of sugarcane cultivation for sugar and bio energy uses.

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Keywords

Genetic diversity, Inter simple sequence repeat (ISSR) marker, Unweighted pair group method with arithmetic average (UPGMA)

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Issue
Vol 8 No 3 (2016)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Genetic variability in sugarcane (Saccharum spp. hybrid) genotypes through inter simple sequence repeats (ISSR) markers. (2016). Journal of Applied and Natural Science, 8(3), 1404-1409. https://doi.org/10.31018/jans.v8i3.973