Bioefficacy of Bacillus subtilis against root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood in tomato
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Abstract
An investigation was conducted for the management of root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood infesting tomato through the application of bio-control agent like Bacillus subtilis, Trichoderma harzianum and Pseudomonas fluorescens. Experiment result revealed that minimum no. of galls/25seedlings (17.50) and maximum seedling height (27.6cm) were observed in Bacillus subtilis @50g/m2 in nursery bed + B. subtilis @ 5kg along with 2.5 tons of FYM/ ha. The highest weight/25seedlings (69.50g) was noticed in the B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. The highest growth of the plant at 45 DAT (49.2cm) and at harvest (81.2cm) and maximum fresh (711.3g) and dry weight (265g) was found in B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha. B. subtilis @50g/m2 in nursery bed + B. subtilis 2.5kg along with 2.5 tons of FYM/ha exhibited lowest gall index (1.2/plant) and highest reduction of nematode population and provided highest yield of tomato fruits (335.75q/ha).
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Bacillus subtilis, Meloidogyne incognita, Tomato
Ashoub, A.H. and Amara, M.T. (2010). Biocontrol activity of some bacterial genera against root-knot nematode, Meloidogyne incognita. Journal of American Sciences. 6: 321-328.
Bina, L., Mohilal, N., Pramodini, M. and Shah, M.M. (2012). Two new species of Boleodorus (Nematoda: Tylenchida) from Manipur, India. The Bioscan., 7 (1): 115-118.
Byrd, D.W., Kirkpatrick, T. and Barker, K.R. (1983). An improved technique for clearing and staining plant tissue for detection of nematodes. Journal of Nematology, 15: 12-143.
Cannayane, I., Rajendran, G. and Ramakrishnan, S. (2001). Biocontrol potential of Bacillus cereus and Bacillus subtilis against Meloidogyne incognita in tomato. Crop Research Hisar, 21 (2): 164-167.
Christie, J.R. and Perry, V.G. (1951). Removing nematode from soil. Proceding of the Helminthological Society of Washington. 18: 106- 108.
Dawar, S., Tariq, M. and Zaki, M.J. (2008). Application of Bacillus species in control of Meloidogyne javanica (Treub) Chitwood on cowpea and mash bean. Pakistan Journal of Nematology, 40: 439-444.
Hussain, M.A., Mukhtar, T. and Kayani, M.Z. (2011). Assessment of the damage caused by Meloidogyne incognita on okra. Journal of Animal and Plant Sciences. 21: 857-861.
Jain, R.K., Mathur, K.N. and Singh, R.V. (2007). Estimation of losses due to plant parasitic nematodes on different crops in India. Indian Journal Nematology, 37: 219-221.
Kayani, M.Z., Mukhtar T. and M.A. Hussain. (2012). Association of root-knot nematodes (Meloidogyne spp.) with cucumber in the Pothowar region of the Punjab province of Pakistan. International Journal of Biology and Biotechnology, 9 (1-2): 23-29.
Khalil, M.S.E.H., Allam, A.F.G.and Barakat, A.S.T. (2012). Nematicidal activity of some biopesticide agents and microorganisms against root-knot nematode on tomato plants under greenhouse conditions. Journal of Plant Protection Research, 52 (1): 47-52.
Mohamedova, M. and Samaliev, H. (2011). Effect of rhizobacterium Bacillus subtilis on the development of the root knot nematode Meloidogyne arenaria at different temperatures. Agricultural Science and Technology, 3(4): 378 - 383
Mokbel, A.A. (2013). Impact of Some Antagonistic Organisms in Controlling Meloidogyne arenaria Infecting Tomato Plants. Journal of Life Sciences and Technologies. 1(1): 69-74.
Mucksood, A.G. and Tabreiz, A.K. (2010). Biological potential of Paecilomyces lilacinus on pathogenesis of Meloidogyne javanica infecting tomato plant. . European Journal of Applied Sciences, 2 (2): 80-84.
Orhan, E., Esitken, A., Ercisli, S., Turan M. and Sahin F. (2006). Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Scientia Horticulturae, 111: 38–43.
Randhawa, N., Sakhuja, P.K. and Singh, I. (2001). Management of root-knot nematode Meloidogyne incognita in tomato with organ-ic amendments. Plant Disease Research. 16: 274-276.
Sakhuja, P.K. and Jain, R.K. (2001). Nematode diseases of vegetable crops and their management. In: “Diseases of Fruits and Vegetables and their Management” (T.S. Thind, ed.). Kaly-ani Pub., Ludhiana, India, pp.474.
Swain, M.R. and Ray, R.C., (2009). Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora. Microbiological Research, 164: 121–130
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