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T. N. Ranjini K. Bhanuprakash M. A. Suryanarayana B. G. Yamuna

Abstract

Terminalia chebula is an important medicinal plant, extensively used in Ayurveda, Unani and Homoeopathic medicines. The present study was aimed to reveal its genetic diversity based on molecular markers from twelve T. chebula accessions. Molecular diversity was studied using RAPD markers. A total of 8 polymorphic primers produced 314 polymorphic bands and 195 monomorphic bands. Unweighted Pair Group Method with Arithmetic Mean (UPMGA) dendrogram divided the accessions into 2 major clusters. Accession IIHRTc2 and IIHRTc10 showed maximum genetic diversity with 55% similarity. This characterization based on molecular markers will help in identification of economically useful accessions for further crop improvement programme.

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Keywords

Dendrogram, Molecular Polymorphism, RAPD, Terminalia chebula

References
Dangi, B., Jain, R., Kachhwaha, S., Kothari, S.L. (2012). Assessment of diversity in Terminalia bellerica Roxb.using morphological, phytochemical and molecular markers. Nat. Acad. Sci. Lett. 35 (1): 135-144.
Deshmukh, V.P., Thakare, P.V., Chaudhari, U.S., Gawande, P.A. and Undal, V.S., (2009). Assessment of genetic diversity among Terminalia species using RAPD markers. Global J. Biotechnol.& Biochem.,4 (2): 70-74.
Kathirvel, A. and Sujatha, V. (2012). In vitro assessment of antioxidant and antibacterial properties of Terminalia chebula Retz. leaves. Asian Pacific J. Tropical Biomedicine. 788-795.
Naik, G.H., Priyadarsini, K.I., Naik, D.B., Gangabhagirathi, R., Mohan, H. (2003). Studies on the aqueous extract of Terminalia chebula as a potent antioxidant and a probable radioprotector. Phytomedicine. 11:530–538.
Powell, W., Morgante, M., Andre, C., Hanafey, M. and Vogel, J., (1996). The comparision of RFLP, RAPD, AFLP, and SSR (microsatellite) markers for analysis, Mol. Breed.,2: 107-111.
Prevost, A. and Wilkinson, M.J., (1999). A new system for compairing PCR primers applied to ISSR fingerprinting of potato cultivars, Theor. Appl. Genet.,98: 107-112.
Roldan-ruiz, I., Dendauw J., Van, B.E., Depicker, A., De, L. M., (2000). AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol. Breed.,6: 125-134.
Sarwat, M., Das, S. and Srivastava, P.S. (2010). Estimation of genetic diversity and evaluation of relatedness through molecular markers among medicinally important trees: Terminalia arjuna, Terminalia chebula and, Terminalia bellerica. Mo.Biol.Rep. 2: 1121-1129.
WEIR, B. S., (1996). Genetic data analysis ??: methods for discreate population genetic data, (Sinauer publishers, Sunderland MA, USA).
Section
Research Articles

How to Cite

Genetic diversity analysis using molecular marker in Terminalia chebula. (2015). Journal of Applied and Natural Science, 7(2), 997-1000. https://doi.org/10.31018/jans.v7i2.720