Sugandh Suman Ranju Kumari V. K. Sharma Harsh Kumar


Isozyme studies of micropropagated and mother plants of banana cvs. Matti, Ney Poovan, Kechulepa, Dwarf Cavendish, Malbhog, Champa, B.B. Battisa and FHIA-1 were done to test their genetic fidelity. The banding patterns as revealed by electrophoretic variations were evaluated with respect to isozymes of acid phosphatase, catalase, esterase and peroxidase as markers. The genetic fidelity of micropropagated plants and the relationship of the different cultivars were determined by dendrogram using numerical taxonomy and multivariate analysis system
(NTSYS). A clustered dendrogram was prepared by unweighted pair group method using averages (UPGAMA) method. At 87% similarity, the micropropagated and mother plants were clustered in four groups reflecting their genomic constitution. Cvs. Matti (AA) and Dwarf Cavendish (AAA) with similar ‘A’ genome were categorized in Cluster I. Cluster II comprised of cvs. Ney Poovan (AB), B.B. Battisa (ABB) and FHIA-1(AAAB) with genomic constitution of both ‘A’ and ‘B’ type. Cvs. Champa (AAB) and Malbhog (AAB) with similar genome were grouped in
Cluster III. Cluster IV contained the cv. Kechulepa (BB) having only ‘B’ genome. However, there was no somaclonal variation among the micropropagated plants and they showed 100% genetic similarity. Thus, the isozyme studies could be a reliable marker for testing the genetic fidelity of micropropagated plants and for evaluating the diversity among the banana germplasm.




Banana, Genetic fidelity, Isozyme, Micropropagation

Anderson, D., Prasad, K. and Stewart, R. (1995). Changes in isozyme Profiles of catalase, peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings. Plant Physiology, 109: 1247 – 1257.
Bhat, K.V., Bhat, S.R. and Chandel, K.P.S. (1997). Survey of isozymes polymorphisms for clonal identification in Musa. Hort - Science, 67: 501-507.
Dice, L.R. (1945). Measures of the amount of ecologic association between species. Ecology, 26: 297-302.
El-Dougdoug, Kh. A., El-Harthi, H.M.S., Korkar, M.M. and Taha, R.M. (2007). Detection of somaclonal variations in banana tissue culture using isozyme and DNA fingerprint analysis. Journal of Applied Sciences Research, 3: 622-627.
Espino, R.R.C. and Pascua G.S. (1992). Isozyme of Philippine banana cultivars/species. Acta Horticulturae, 1: 186 -190.
Horry, J.P. and Jay, M. (1988). Distribution of anthocyanins in wild and cultivated banana varieties. Phytochemistry, 27: 2667-2672.
Izquierdo, H., González, Maria. C. and de la C. Nunez., Miriam. (2014). Genetic stability of micropropagated banana plants (Musa spp.) with non-traditional growth regulators. Biotecnología Aplicada, 31: 23-27.
James, A.C., Echeverria, S.P., Echeverria, L.P. and Valenci, V.A.H. (2007). Variation in micropropagated plants. Acta Horticulturae, 748: 55-63.
Jarret, R.L. and Litz, R.E. (1986). Enzyme polymorphism in Musa acuminata Colla. Journal of Heredity, 77: 183 -188.
Karamura, D.A. (1998). Numerical taxonomic studies of the East African highland bananas (Musa AAA-East Africa) in Uganda. INIBAP, France, pp 192.
Larkin, P.J. and Scowcroft, W.R. (1981). Somaclonal variation - a novel source of variability from cell culture for plant improvement. Theoretical and Applied Genetics, 60: 197-214.
Lebot, V., Aradhya, K.M., Manshardt, R. and Meilleur B. (1993). Genetic relationships among cultivated bananas and plantains from Asia and the Pacific. Euphytica, 67: 163–173.
Liyanage, A.S.U., Manawaprema, M.M.C. and Mendis, M.H. (1998). Differentiation of A & B genome of banana and plaintain (Musa spp.) by esterase enzyme. Journal of the National Science Council of Sri Lanka, 26: 125-131.
Mendioro, M.S., Madriñan, S.L., Colcol, F.J. and Dela Cruz, F.S. (2007). Isozyme polymorphism of selected cultivars of table and cooking-type banana (Musa sp.) in the Philippines and genome identification of the table -type cultivars. Philippine Journal of Science, 136: 45-56.
Murashige, T. and Skoog, E. (1962). A revised medium for rapid growth and bioassay with tobacco cultures. Journal of Plant Physiolology, 15: 473-479.
Panara, F., Pasqualini, S. and M. Antonielli (1990). Multiple forms of barley root acid phosphatase: Purification and some characteristics of major cytoplasmic isoenzyme. Biochimica et Biophysia, Acta, 1037: 73-80.
Pancholi, N.C., Wetten, A. and Caligari, P.D.S. (1996). Detection of levels of somaclonal variation in Musa using molecular markers. Meeting on tropical plants. 11-15. March. Montpellier, pp 2.
Rao, Y.S., Manga, V. and Rao, M.V.S. (1972). Developmental variation and tissue specificity of nine isozymes in pearl millet. Crop Improvement, 19: 75-80.
Richardson, D.L., Hamilton, K.S. and Hutchinson, D.J. (1965). Notes on banana.1-Natural edible tetraploids. Tropical Agriculture, 42: 125-137.
Sahijram, L. Soneji, J.R. and Bollamma, K.T. (2003). Analyzing somaclonal variation in micropropagated bananas (Musa spp.). In vitro Cellular and Developmental Biology Plant, 39: 551-556.
Shield, C.R., Orton, T.J. and Stuber, C.W. (1983). An outline of general resource needs and procedures for electrophoretic separation of active enzymes from plant tissues. In: Tanksley SD and Orten TJ (Eds.), Isozymes in Plant Genetic and Breeding. Elsevier, Amsterdam, pp 443-468.
Simmonds, N.W. (1962). Evolution of the Bananas. Longman, Green and Co. Ltd., London, UK, pp 170.
Stover, R.H. and Simmonds, N.W. (1987). Bananas. 3rd edition. Longmans, Scientific and Technical, Essex, England, pp 468.
Suman, S., Rajak, K.K. and Kumar, H. (2013a). Micropropagation of banana cv. B.B. Battisa. Biochemical and Cellular Archieves,13: 249-254.
Suman, S., Rajak, K.K., Kishore, C. and Kumar, H. (2013b). Micropropagation of banana cv. Champa. Biochemical and Cellular Archieves, 13: 291-295.
Venkatachalam, L., Sreedhar, R.V. and Bhagyalakshmi, N. (2008). The use of genetic markers for detecting DNA polymorphism, genotype identification and phylogenetic relationships among banana cultivars. Molecular Phylogenetics and Evolution, 47: 974-985.
Vincent, J.B., Crowder, M.W. and Averill, B.A. (1992). Hydrolysis of phosphate monoester: a biological problem with multiple chemical solutions. Trends in Biochemical Sciences, 17: 105-110.
Research Articles

How to Cite

Isozyme analysis based genetic fidelity assessment of micropropagated banana plants. (2015). Journal of Applied and Natural Science, 7(2), 579-584. https://doi.org/10.31018/jans.v7i2.648