Prevalence, serodiversity and antibiogram of enterotoxigenic Escherichia coli (ETEC) in diarrhoeic calves and lambs of Kashmir valley (J&K), India
##plugins.themes.bootstrap3.article.main##
Abstract
Enterotoxigenic E. coli (ETEC) is the major cause of diarrhoea in neonatal animals. This study determined the prevalence, serological diversity, virulence gene profile and in-vitro antibiogram of ETEC isolates from diarrhoeic faeces of calves and lambs. The prevalence rate of ETEC in lambs was recorded 18.46 % with O8 as predominant serotype. However, in calves the prevalence rate was recorded 8.57 % with O15 and O26 as predominant serotypes. The antibiogram screening showed differential susceptibility pattern among ETEC isolates with highest resistance to ampicillin and highest sensitivity to enrofloxacin. In the present study, for the first time it was reported that the diarrhoea in calves and lambs occur due to virulent gene est not due to elt gene, which was absent in all the isolates.
##plugins.themes.bootstrap3.article.details##
##plugins.themes.bootstrap3.article.details##
Keywords
Calves, Diarrhoea, Lambs, PCR
References
Blanco, J., Cid, D., Blanco, E., Blanco, M., Ruiz, J., Quiteira, S.and Fuente, R. (1996). Serogroups, toxins and antibiotic resistance of Escherichia coli strains isolated from diarrhoeic lambs in Spain. Veterinary Microbiology, 18 :209-217.
Bradford, P.A., Petersen, P.J., Fingerman, I.M. and White, D.G. (1999). Characterization of expanded-spectrum cephalosporin resistance in E. coli isolates associated with bovine calf diarrhoeal disease. J. Antimicrobial Chemotherapy, 44:607-610.
Buchanan, R.E. and Gibbon, N.E. (1994). Bergey’s Manual of Determinative Bacteriology. 9th edition. Williams and Wilkins, Baltimore, pp787.
Croxen, M.A., Law, R.J., Scholz, R., Keeney, K.M., Wlodarska, M., and Finlay, B.B. (2013). Recent advances in understanding enteric pathogenic Escherichia coli. Clin. Microbiol. Rev., 26: 82–880.
Darong, C., Yuan, Z.S., Lang, C.X., Pan, G.X., Wei, D.W. and Chang, S.H. (2010). Rapid diagnosis of ETEC and HPI-harboring Escherichia coli infection in newborn piglets with diarrhoea. African Journal of Microbiology Research, 4(7)-:575-580.
Gyles, C.L. (1992). Escherichia coli cytotoxins and enterotoxins. Can J Microbiol., 38:734-46.
Itoh, F., Ogino, T., Itoh, F. and Watanabe, H. (1992). Differentiation and detection of pathogenic determinants among diarrhoeagenic Escherichia coli by polymerase chain reaction using mixed primers. Jpn. J. Clin. Med., 50: 343-347.
Kolenda, R., Burdukiewicz, M. and Schierack, P (2015). A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front. Cell. Infect. Microbiol.5: 23.
Myers, L.L. (1975). Characterization of Escherichia coli obtained from newborn calves with diarrhoea. Infection and Immunity, 11: 493-496.
Orskov, F. and Orskov, I. (1992). Escherichia coli serotyping and disease in man and animals. Can. J.Microbiol., 38: 699-704.
Philipp, R., Weiglmeier,M., Rösch, P and Berkner, H. (2010). Cure and Curse: E. coli Heat-Stable Enterotoxin and Its Receptor Guanylyl Cyclase C. Toxins, 2: 2213-2229.
Sambrook, J., Russel, D.W. (2001). Molecular Cloning - A Laboratory Manual. Cold Spring Harbour, New York, USA.
Seh, D.K., Soni, S.S., Kashyap, S.K. and Shringi, B.N. (2000). Seroprevalence, antibiotic sensitivity pattern and transfer of plasmid coded characters of E. coli. Indian Vet. J., 81: 6-8.
Sivaswamy, G. and Gyles, C.L. (1976). The prevalence of enterotoxigenic Escherichia coli in the faeces of calves with diarrhoea. Canad. J. Comp. Med., 40: 241-246.
Tripathi, R.D. and Soni, J.L. (1984). Enteropathogenic E. coli in neonatal calf diarrhoea in crossbred calves. Indian Vet. J., 61: 4-8.
Wani, S.A., Bhat, M.A., Munshi, Z.H., Qureshi, S. and Buch, A.S. ( 2003). Isolation and in-vitro sensitivity pattern of pathogenic Escherichia coli from diarrhoeic lambs and calves. Journal of Animal Sciences, 73 (2): 168-170.
Wani, S.A., Hussain, I., Beg, S.A., Rather, M.A., Kabli, Z.A., Mir, M.A. and Nishikawa, Y.(2013). Diarrhoeagenic Escherichia coli and Salmonellae in calves and lambs in Kashmir. absence, prevalence and antibiogram. Rev. sci. tech. Off. Int. Epiz., pp 32(3).
Wray, C. and Thomlinson, J.R. (1991). Factors influencing occurrence of colibacillosis in calves. Veterinary Record., 96(3): 52-56.
Bradford, P.A., Petersen, P.J., Fingerman, I.M. and White, D.G. (1999). Characterization of expanded-spectrum cephalosporin resistance in E. coli isolates associated with bovine calf diarrhoeal disease. J. Antimicrobial Chemotherapy, 44:607-610.
Buchanan, R.E. and Gibbon, N.E. (1994). Bergey’s Manual of Determinative Bacteriology. 9th edition. Williams and Wilkins, Baltimore, pp787.
Croxen, M.A., Law, R.J., Scholz, R., Keeney, K.M., Wlodarska, M., and Finlay, B.B. (2013). Recent advances in understanding enteric pathogenic Escherichia coli. Clin. Microbiol. Rev., 26: 82–880.
Darong, C., Yuan, Z.S., Lang, C.X., Pan, G.X., Wei, D.W. and Chang, S.H. (2010). Rapid diagnosis of ETEC and HPI-harboring Escherichia coli infection in newborn piglets with diarrhoea. African Journal of Microbiology Research, 4(7)-:575-580.
Gyles, C.L. (1992). Escherichia coli cytotoxins and enterotoxins. Can J Microbiol., 38:734-46.
Itoh, F., Ogino, T., Itoh, F. and Watanabe, H. (1992). Differentiation and detection of pathogenic determinants among diarrhoeagenic Escherichia coli by polymerase chain reaction using mixed primers. Jpn. J. Clin. Med., 50: 343-347.
Kolenda, R., Burdukiewicz, M. and Schierack, P (2015). A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front. Cell. Infect. Microbiol.5: 23.
Myers, L.L. (1975). Characterization of Escherichia coli obtained from newborn calves with diarrhoea. Infection and Immunity, 11: 493-496.
Orskov, F. and Orskov, I. (1992). Escherichia coli serotyping and disease in man and animals. Can. J.Microbiol., 38: 699-704.
Philipp, R., Weiglmeier,M., Rösch, P and Berkner, H. (2010). Cure and Curse: E. coli Heat-Stable Enterotoxin and Its Receptor Guanylyl Cyclase C. Toxins, 2: 2213-2229.
Sambrook, J., Russel, D.W. (2001). Molecular Cloning - A Laboratory Manual. Cold Spring Harbour, New York, USA.
Seh, D.K., Soni, S.S., Kashyap, S.K. and Shringi, B.N. (2000). Seroprevalence, antibiotic sensitivity pattern and transfer of plasmid coded characters of E. coli. Indian Vet. J., 81: 6-8.
Sivaswamy, G. and Gyles, C.L. (1976). The prevalence of enterotoxigenic Escherichia coli in the faeces of calves with diarrhoea. Canad. J. Comp. Med., 40: 241-246.
Tripathi, R.D. and Soni, J.L. (1984). Enteropathogenic E. coli in neonatal calf diarrhoea in crossbred calves. Indian Vet. J., 61: 4-8.
Wani, S.A., Bhat, M.A., Munshi, Z.H., Qureshi, S. and Buch, A.S. ( 2003). Isolation and in-vitro sensitivity pattern of pathogenic Escherichia coli from diarrhoeic lambs and calves. Journal of Animal Sciences, 73 (2): 168-170.
Wani, S.A., Hussain, I., Beg, S.A., Rather, M.A., Kabli, Z.A., Mir, M.A. and Nishikawa, Y.(2013). Diarrhoeagenic Escherichia coli and Salmonellae in calves and lambs in Kashmir. absence, prevalence and antibiogram. Rev. sci. tech. Off. Int. Epiz., pp 32(3).
Wray, C. and Thomlinson, J.R. (1991). Factors influencing occurrence of colibacillosis in calves. Veterinary Record., 96(3): 52-56.
Issue
Section
Research Articles
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
How to Cite
Prevalence, serodiversity and antibiogram of enterotoxigenic Escherichia coli (ETEC) in diarrhoeic calves and lambs of Kashmir valley (J&K), India. (2015). Journal of Applied and Natural Science, 7(1), 477-481. https://doi.org/10.31018/jans.v7i1.635
