Variability in yield traits of TILLING population of bread wheat (Triticum aestivum L.)
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Abstract
Mutagenesis is one of the powerful genetic strategies for crop improvement programmes. A chemically induced mutated genetic resource for detecting novel variations by Targeting Induced Local Lesions IN Genomes (TILLING) has been developed in recently released bread wheat (Triticum aestivum) cultivar DPW621-50. A total of 3,478 M2 plants were evaluated for plant height, number of tillers/plant, thousand grain weight, number of seeds/spike and grain yield/plant. A large variation was observed for all the traits. The highest frequency (52.2%) of lines had similar height between 91-100 cm to the non-mutagenized DPW 621-50 control followed by 28.9% of lines with height between 81-90 cm. A large variation was observed in number of tillers/plant which ranged from 1-35 tillers/plant. The highest frequency (32.09%) lines had 31-40 seeds/spike followed by 29.84% lines with 41-50 seeds/spike. Few lines (0.35%) had more than 70 seeds/spike with normal seed size as their thousand grain weight ranged between 34.82-43.82g. Chlorophyll deficient, grassy type and sterile mutants were also observed. This population may serve as new genetic resource for functional genomics studies and novel variants for different traits in elite germplasm can be made available to the plant breeders for wheat improvement.
Article Details
Article Details
Mutagenesis, TILLING, Triticum aestivum
Chen, A. and Dubcovsky, J. (2012). Wheat TILLING mutants show that the vernalization gene VRN1 down-regulates the flowering repressor VRN2 in leaves but is not essential for flowering. Plos Genetics, 8: e1003134.
Chen, L., Huang, L., Min, D., Phillip, A., Wang, S., Madgwick, P.J., Parry, M.A. and Hu, Y.G. (2012). Development and characterization of a new TILLING population of common bread wheat (Triticum aestivum L). PLos ONE 7(7): e41570.
Dong, C., Dalton-Morgan, J., Vincent, K. and Sharp, P. (2009). A modified TILLING method for wheat breeding. Plant Genome, 2:39-47.
Feiz, L., Beecher, B.S., Martin, JM. and Giroux, M.J. (2009). In planta mutagenesis determines the functional regions of the wheat puroindoline proteins. Genome, 183:853-860.
Gepts, P. and Papa, R. (2002). Evolution during domestication. Encyclopedia of Life Sciences, :1–7.
Guttieri, M., Bowen, D., Dorsch, J.A., Raboy, V. and Souza, E. (2004). Identification and characterization of a low phytic acid wheat. Crop Science, 44:418-424
Laudencia-Chingcuano, D. (2012). Isolation and characterization of EMS-induced Dy10 and Ax1 high molecular weight glutenin subunit deficient mutant line of elite hexaloid wheat (Triticum aestivum L.) cv Summit. Journal of Cereal Science, 56:296-299.
Parry, M.A.J., Madgwick, P.J., Bayon, C., Tearall, K., Hernandez-Lopez, A., Baudo, M., Rakszegi, M., Hamada, W., Al-Yassin, A., Ouabbou, H., Labhilili, M. and Phillips, A.L. (2009). Mutation discovery for crop improvement. Journal of Experimental Botany, 60: 2817-2825.
Rawat, N., Sehgal, S.K., Joshi, A., Rothe, N., Wilson, D.L., McGraw, N., Vadlani, P.V., Li, W. and Gill, B.S. (2012). A diploid wheat TILLING resource for wheat functional genomics. BMC Plant Biology, 12:205.
Schramm, E.C., Nelson, S.K. and Steber, C.M. (2012). Wheat ABA-insenstive mutants result in reduced grain dormancy. Euphytica, 188:35-49.
Slade, A.J., Fuerstenberg, S.I., Loeffler, D., Steine, M.N. and Facciotti, D. (2005). A reverse genetic, nontransgenic approach to whet improvement by TILLING. Nature Biotechnology, 23:75-81.
Slade, A.J. and Knauf V.C. (2005). TILLING moves beyond functional genomics into crop improvement. Transgenic Research, 14:109-115.
Slade, A.J., McGuire, C., Loeffler, D., Mullenberg, J., Skinner, W., Fazio, G., Holm, A., Brandt, K.M., Steine, M.N., Goodstal, J.F. and Knauf, V.C. (2012). Development of high amylose wheat through TILLING. BMC Plant Biology, 12:69.
Uauy, C., Paraiso, F., Colasuonno, P., Tran, R.K., Tsai, H., Berardi, S., Comai, L. and Dubcovsky, J. (2009). A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biology, 9:115.
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