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Milind B. Katare N. Basavaraja Harshavardhan D. Joshi Chirom Archana

Abstract

The aim of this study was to evaluate the effect of letrozole (a non-steroidal aromatase inhibitor) on masculinization of Siamese fighting fish (Betta splendens). Different doses of letrozole 50, 100, 150 and 200 ppm (mg/kg feed) were incorporated into diet and fed for periods of 30, 40 or 50 days. Immersion treatment of letrozole at selected doses (250, 500, 750 and 1,000?g/l) for 3 h each on third, fifth and eighth day-post-hatching (dph) (Trial 1) and fourth, sixth and eighth dph (Trial 2) was given.The oral administration of letrozole for 30, 40 or 50 days did not have any significant effect on the sex ratio of B. splendens. The immersion treatment of letrozole induced 100% masculinization at 500 and 1000?g/l in trial 1, while it yielded only 66.66 and 90% male population at 500 and 1000 ?g/l in Trial 2. The dietary administration of letrozole for 30 and 50 days caused tail deformities and/or rudimentary and absence of dorsal fin. The progeny testing of males from letrozole treated (both oral and immersion) groups indicate that the sex ratio of progenies of each of the males tested did not differ significantly from that of control, indicating that all those males carried XY genotype. Letrozole treatments suppressed ovarian development (atretic oocytes were common). However, the testicular development was unaffected. The study revealed that immersion treatment of letrozole was more effective in inducing masculinization of B. splendens than the oral administration of letrozole.

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Keywords

Aromatase inhibitor, Deformity, Fighting fish, Masculinization, Histology

References
Afonso, L.O.B., Wassermann, G.J. and Oloveira, R.T.D.(2001). Sex reversal in Nile tilapia (Oreochromis niloticus) using a nonsteroidal aromatase inhibitor. J. Exp. Zool., 290:177-181.
Balasubramani, A. and Pandian, T.J. (2008). Norethindrone ensures masculinization, normal growth and secondary sexual characteristics in the fighting fish, Betta splendens. Curr.Sci., 95(10): 1446-1453.
Basavaraja, N., Chandrashekhara, B.H. and Rather, M.A. (2012). Production of an all-male population of guppy, Poecilia reticulate (Schneider). Curr. Sci., 11:1151-1152.
Chandrashekhara, B.H. (2011). Production of all-male population of Lebistes reticulates (Robert John Lechmn,1866) through the application of aromatase inhibitor, MFSc thesis, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar.
Das, R., Rather, M.A., Basavaraja, N., Sharma, R. And Udit, U.K. (2012). Effect of nonsteroidal aromatase inhibitor on sex reversal of Oreochromis mossambicus (Peters, 1852). Isr. J. Aquacult. – Bamidgeh, 64: 1-6.
Fenske, M. and Segner, H. (2004). Aromatase modulation alters gonadal differentiation in developing zebra fish (Danio rerio). Aquat. Toxico., 67:105-126.
Finn, R.N. and Kapoor, B.G. (2007). Fish Larval Physiology, Science Publishers, New Hampshire.
Gao, Z.X., Wang, H.P., Wallat, G., Yao, H., Rapp, D., O’bryant, P., MacDonald and Wang, W.M. (2010). Effects of a nonsteroidal aromatase inhibitor on gonadal differentiation of bluegill sunfish, Lepomis macrochirus. Aquacult. Res., 41: 1282.
Gray, P. (1964). Handbook of Basic Microtechniques. McGraw-Hill Book Company. 3rd ed, New York.
Guerrero, R.D. (1975). Use of androgens for the production of all-male Tilapia aurea. Transactions of the American Fisheries society., 104:342-348.
Howell, A., Cuzick, J., Baum, M., Buzdar, A., Dowsett, M., Forbes, J.F., Hoctin-Boes, G., Houghton, J., Locker, G.Y. and Tobias, J.S. (2005). Results of the ATAC (arimidex, tamoxifen, alone or in combination) trial after completion of 5 years' adjuvant treatment for breast cancer. Lancet., 365: 60-62.
Jessy, D. and Varghese, T.J. (1988). Hormonal sex control in Betta splendens (Regan) and Xiphophorus helleri (Heckel), Paper presented to First Indian Fisheries Forum, Asian Fisheries Society, Indian Branch, Mangalore, India, pp 26-31.
Kavumpurath, S. and Pandian, T.J. (1993). Determination of labile period and critical dose for sex reversal by oral administration of estrogens in Betta splendens Regan. Indian. J. Exp. Biol., 31:16-20.
Kavumpurath, S. and Pandian, T.J. (1994). Masculinization of fighting fish, Betta splendens Regan, using synthetic or natural androgens. Aquacutlt Fish Manage., 25: 373-381.
Kipouros, K., Paschos, I., Gouva, E., Ergolavou, A.and Perdikaris, C. (2011). Masculinization of the ornamental Siamese fighting fish with oral hormonal administration. Sci. Asia., 37:277-280.
Kirankumar, S. and Pandian, T.J. (2002). Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens. J. Exp. Zool., 293: 606-616.
Kitano, T., Takamune, K., Nagahama, Y. and Abe, S.I. (2000). Aromatase inhibitor and 17alpha-methyltestosterone cause sex-reversal from genetical females to phenotypic males and suppression of P450 aromatase gene expression in Japanese flounder (Paralichthys olivaceus). Mol. Reprod. Dev., 56: 1-5.
Kwon, J.Y., Haghpanah, V., Kogson-Hurtado, L.M., McAn drew, B.J. and Penman, D.J. (2000). Masculinization of genetic female Nile tilapia (Oreochromi sniloticus) by dietary administration of an aromatase inhibitor during sexual differentiation.J. Exp. Zool., 287:46-53.
Li, G.L., Liu, X.C. and Lin, H.R. (2005). Aromatase inhibitor letrozole induces sex inversion in the protogynous red spotted grouper (Epinephelus akaara). Acta. Physiol. Sin., 57: 473-479.
Pandian, T.J. and Sheela, S.G. (1995). Hormonal induction of sex reversal in fish. Aquaculture, 138 :1-22.
Person, L.R.J., Alexandre, J.C., Thebaud, L.andMugnier, C. (1993).Marine fish larvae feeding: Formulated diets or live prey. J. World. Aquacult. Soc., 24 (2): 211-224.
Piferrer, F., Zanuy, S., Carrillo, M., Solar, I.I., Devkubm, R. H. and Donaldson, E.M. (1994). Brief treatment with an aromatase inhibitor during sex differentiation causes chromosomallyfemale salmon to develop as normal, functional males. J .Exp. Zool., 270:255-262.
Salam, A., Ai-Ablani, Ronald. P. and Phelps (2001). Induction of feminization in large mouth bass (Micropterus salmoides) by oral administration of estradiol-17? or diethylstilbestrol and associated Pathological changes. J. Aquacult. Trop., 16 (2):185-195.
Sales, J. and Janssen, G.P.J. (2003). Nutrient requirements of ornamental fish. Aquat. Liv. Resour., 16: 533-540.
Steele, R.E., Mellor, L.B., Sawyer, W.K., Wasvary, J.M. and Browne, L.J. (1987). In vitro and in vivo studies demonstrating potent and selective estrogen inhibition by the non-steroidal aromatase inhibitor CGS16949. Steroids., 50: 147-161.
Sun, L.W., Zha, J.M., Spear, P.A. and Wang, Z.J.(2007). Toxicity of the aromatase inhibitor letrozole to Japanese medaka (Oryzias latipes) eggs, larvae and breeding adults. Comp. Bioche. Physiol., 145: 533-541.
Vasanthakumar, A., Basavaraja, N., Manjappa, N. and Vinod, B.H. (2011). Manipulation of sex and progeny testing in common carp, Cyprinus carpio(Linn.). J. Aquacult. Trop., 26:63-81.
Vinod, B.H. and Basavaraja, N. (2010). Effect of diethylstilbestrol and norethindrone on sex ratio and body indices of common Carp, Cyprinus carpio (Linn.). Asian Fish Sci., 23: 100-115
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Katare, M. B., Basavaraja, N., Joshi, H. D., & Archana, C. (2015). Effect of letrozole on masculinization of Siamese fighting fish (Betta splendens). Journal of Applied and Natural Science, 7(1), 425–433. https://doi.org/10.31018/jans.v7i1.627
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