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M. Vindyashree M. R. Govindappa V. N. Ghante D. S. Aswathanarayana I. Shankergoud

Abstract

The present study was conducted to identify the alternate hosts of new leaf curl virus disease of sunflower. In the present study several crops and weed hosts were cross inoculated with leaf curl virus of sunflower under laboratory through insect vector whitefly (Bemisia tabaci), further all inoculated samples were retested (3-4 weeks after inoculation) by molecular based Polymerse chain reaction diagnosis for the presence of virus. The results revealed that the causal virus of the disease was successfully transmitted from sunflower to sunflower (Helianthus annuus), tomato (Solanum lycopersicum) and tobacco (Nicotiana tabacum L) and weed hosts such as Acanthospermum hispidum, Amaranthus viridis and Parthenium hysterophorus in a short incubation period (2-3 weeks after inoculation), while on other hosts Chilli (Capsicum annuum L) and Datura stramonium, infection occurs in delayed incubation period. Further molecular analysis thorough polymerase chain reaction (PCR) diagnostic technique using virus specific primers also confirmed the presence of coat protein (CP) of leaf curl begomovirus in
virus inoculated hosts viz., chilli, sunflower, tomato, and tobacco and weed hosts such as Acanthospermum hispidum, Amaranthus viridis, Datura stramonium and Parthenium hysterophorus. Thus, findings substantiate that the above hosts are major sources of the virus inoculum and served as potential alternate hosts of the disease during the off season.

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Keywords

Begomovirus, Host range, Polymerase chain reaction, Sunflower, Whitefly

References
Anonymous (2013). Sunflower Annual Report, All India Coordinated Research Project on Sunflower, Directorate of Oilseed Research, Rajendranagar, Hyderabad, pp 359.
Briddon, R. W., Bull, S. E., Mansoor, S, Amin, I. and Mark-ham, P. G. (2002). Universal primers for the PCR-mediated amplification of DNA beta: a molecule associ-ated with some monopartite begomoviruses, Mol. Bio-technol., 20(3): 315-318
Brown, J. K., Idris A. M., Torres-Jerez, I., Banks, G. K. and Wyatt, S. D. (2001). The core region of the coat protein gene is highly useful in establishing the provisional identification and classification of begomoviruses. Arch. Virol., 146: 1581-1598.
Chowda Reddy, R. V., Colvin, J., Muniyappa, V. and Seal, S. (2005). Diversity and distribution of begomoviruses infecting tomato in India. Arch. Virol.150: 845-867.
Colvin, J., Omango, C. A., Govindappa., M. R., Stevenson, P. C., Maruthi, M. N., Gibson, G., Seal, S.E., and Muni-yappa, V. (2006). Host plant Viral infection effects on arthropod vector population growth, development and behavior: Management and epidemiological implica-tions, Advances in Virus research, (67) :420-453.
Govindappa, M. R., Shankergoud, I., Shankarappa, K. S., Wickramaarachchi, W. A. R. T., Anjeneya Reddy, B. and Rangaswamy, K. T. (2011). Molecular detection and partial characterization of begomovirus associated with leaf curl disease of sunflower (Helianthus annuus) in Southren India. Plant Pathology Journal, 10(1):29-35.
Lodhi, M. A., Ye, G. N., Weeden, N. F. and Reisch, B. (1994.) A simple and efficient method for DNA extrac-tion from grapevine cultivars and Vitis species. Pl. Mol. Biol. Rep., 12: 6-13.
Mahesh, Y. S., Shankarappa, K. S., Rangaswamy, K. T., Prameela, H. A., Aswathanarayana, D. S., Divya, B. L., Nagesha, N., and Maruthi, M. N. (2010). Detection and characterization of a Begomovirus associated with leaf curl disease of ornamental croton (Codiaeum variega-tum). J. Hort. Sci. Biotech., 85: 101-105.
Maruthi, M. N., Rekha, A. R. and Muniyappa, V. (2007). Pumpkin yellow vein mosaic disease is caused by two distinct begomoviruses: complete viral sequences and comparative transmission by an indigenous Bemisia tabaci and the introduced B- biotype. EPPO Bull., 37 (2): 412-419.
Muhammad, W. A., Muhammad S. A. and Hammad, N. T, (2012). Combining ability analysis for achene yield and related traits in sunflower (Helianthus annuus L.). Chil-ean Journal of Agricultural Research., 72(1): 21-26.
Muniyappa, V., Venkatesh, H. M., Ramappa, H. K., Kul-karni, R. S., Zeidan, M., Tarba, C. Y., Ghanim, M. and Czosnek, H. (2000). Tomato leaf curl virus from Banga-lore (ToLCV-Ban4): sequence comparison with Indian ToLCV isolates, detection in plants and insects, and vector relationships. Arch. Virol., 145: 1583–1598.
Rajeshwari, R., Reddy, R. V. C., Maruthi, M. N., Colvin, J., Seal, S. E. and Muniyappa, V. (2005). Host range, vec-tor relationships and sequence comparison of a bego-movirus infecting hibiscus in India. Annals of Applied Biology, 147: 15-25.
Ramappa, H. K., Muniyappa, V. and Colvin, J. (1998). The contribution of tomato and alternative host plants to tomato leaf curl virus inoculums pressure in different areas of south India. Annals of Applied Biology, 88: 369-387.
Ramaiah, M., Bhat, A. I., Jain, R. K., Pant, R. P., Ahlawat, Y. S., Prabhakar, K. and Varma, A. (2001). Partial char-acterization of an Isometric virus causing Sunflower Necrosis Disease. Indian Phytopathology, 54: 246-250
Saharan, G. S., Naresh Mehta. and Sangwan, M. S. (2005). Diseases of oil seed crops, ISBN-81-7387-176-0, Indus publishing company, New Delhi, pp.38.
Sharma, R. K., Gupta, V. K., Jindal. J. and Dilawari, V. K. (2008). Host associated genetic variations in whitefly Bemisia tabaci (Genn.), Indian J. Biotechnology, 7: 366-370.
Vanitha, L.S., Shankarappa, K.S., Rangaswamy, K.T., Wick-ramaarachchi W.A.R.T. and Govindappa, M.R. (2013). Complete Nucleotide Sequence of Tomato Leaf Curl Karnataka Virus and β Satellite Molecule Associated with Leaf Curl Disease on Sunflower in India. Plant Pathology Journal, 12: 19-25.
Varma, A. and Malathi, V. G. (2003). Emerging geminivirus problems: A serious threat to crop production. Annals of Applied Biology, 142: 145-164.
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How to Cite
Vindyashree, M., Govindappa, M. R., Ghante, V. N., Aswathanarayana, D. S., & Shankergoud, I. (2015). Biological and molecular evidences on host range of leaf curl begomovirus disease of sunflower (Helianthus annuus L.). Journal of Applied and Natural Science, 7(1), 381-387. https://doi.org/10.31018/jans.v7i1.620
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