Aegle marmelos (L.) corr. commonly known as ‘Bael’ tree is included in the red- listed medicinal tree in South India. Riped and unripe fruits, root, bark and leaves of the tree are used in ayurvedic preparation for various ailments. This study was conducted to standardize the media for multiple shoot induction from different explants under in vitro condition. Among the various explants viz., meristematic tip, nodal bud, tender leaf, auxillary bud and cotyledon tried in different media, auxiliary bud had shown good response for shoot induction in 36 to 39 days. Among the various treatments viz., WPM supplemented with Benzyl Amino Purine (BAP) (0.5 to 3.0mg/lit), Kinetin (KN) (0.5 to 3mg/lit) and Indole acetic acid (IAA) (0.5 to 2mg/lit) for multiple shoot induction, the treatmental combination of WPM supplemented with BAP, KN and IAA, @ of each 0.5 mg/lit resulted in 7.75 shoots per cotyledon. The same combination, ½ strength of the medium with 1mg/lit BAP+2mg/lit NAA+ 1% AC resulted in root formation. Hence, as a means of in vitro conservation using cotyledon as explants microproagation is possible in bael.
Bael, hormonal combination, multiple shoot, Shoot induction, Root induction
Hazeena, M.S. and G.R. Sulekha. (2008). Callus induction and plantlet regeneration in Aegle marmelos (L.) Corr. using cotyledon explants. Journal of Tropical Agriculture., 46 (1-2): 79–84.
Kalamani, A. and S. Michael Gomez (2002). In vitro propagation studies in Clitoria spp. International Journal Mendel, 19(2):29-30.
Lloyd, G. and B. McCown. (1980). Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Proc. Intl. Plant Prop. Soc., 30: 421-427.
Murashige, T. and Skoog, F. (1962): A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473-495.
Pati. and M. Muthukumar. (2013). Genetic Transformation of Bael (Aegle marmelos. Corr.) Biotechnology of Neglected and Underutilized Crops, DOI: 10.1007/978-94-007-5500-0_14.
Pradeepa Devi, C.B., R.M. Gopal and A. Settu. (2014). Plant regeneration of Aegle marmelos (L.) corr. from cotyledon explants through in vitro studies. J. Nat. Prod. Plant Resour., 4 (2):52-55.
Purohit, S.D., A. Dave, and G. Kukda. (1995) Micropropagation of safed musli (Chlorophytum borivilianum), a rare medicinal herb. Plant Cell, Tissue and Organ Culture, 39: 93-96.
Puhan, Puspashree P. and Rath, S.P. (2012). In vitro propagation of Aegle marmelos (L.) Corr., a medicinal plant through axillary bud multiplication. Advances in Bioscience and Biotechnology, 3: 121-125
Ravikumar, K. and. K. Ved. (2000). Aegle marmelos, 100 Red – Listed medicinal plants of Conservation Concern in Southern India., Foundation for Revitalisation of Local Health Traditions, Bangalore. pp: 41-43.
Rekha Warrier., Jasmine Viji and P. Priyadharshini. (2010). In Vitro Propagation of Aegle Marmelos L. (Corr.) from mature trees through enhanced axilliary brancing. Asian J. Exp. Biol. Sci., 1 (3): 669-676.
Shailajan, S., S. Menon and H. Hande. (2011). Method validation of Marmelosin from fruit pulp of Aegle marmelos (L.) Correa using HPTLC Technique. J. Pharm. Res., 4(5):1353–1355.
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