Sunil Kumar Virendra Kumar Manoj Kumar Sharma Narendra Kumar Anil Kumar Kushal Pal Singh Tomar Sarjeet Kumar Sharma Mukesh Kumar Singh R. S. Sengar Nidhi Jaiswal


Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical and subtropical parts of the world. The present study was undertaken to investigate the effects of different plant growth regulators on in vitro callus induction in physic nut (J. curcus). In the present study, it was observed that all the explants viz., leaf lamina, petioles, nodal segments and cotyledonary nodes showed good callus induction responses on various culture media thus tried. Leaf lamina and petioles showed 100.0% callus induction responses on different MS media supplemented with auxins and cytokinins alone or in combinations whereas, nodal segments and cotyledonary nodes showed maximum 89.6% and 83.9% callus induction respectively. The presence of 2, 4-D in culture media with auxins or cytokinins was essential for good callus growth. Among different explants tried, leaf lamina was the best responding explants and MS-13 media supplemented with 5×10-6 M NAA and 10-5 M 2, 4-D is the best callusing and growth supporting medium. However, the regenerative competence of the callus tissues can differ depending on the type of explants used because certain types of plant tissues have more favorable regeneration responses than others. Callus induction rate from all explant types was highest than other reports. The results obtained in the present study would facilitate the high callus induction and regeneration responses in J. curcus for its improvement using biotechnological tools.




Auxins, Callus, Jatropha, Plant regeneration, Tissue culture

Abdulla, R., Chan, E.S. and Ravindra, P. (2011). Biodiesel production from Jatropha curcas: a critical review. Critical Reviews in Biotechnology, 31:53-64.
Deore A.C. and Johnson T.S. (2008). High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotech. Reporter, 2:10-15.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp. Cells Research, 50:151-204.
Jose, J., Nimisha, K., Anu M.A. and Nambisan, P. (2012). Evaluation of somaclonal variation in callus cultures of Jatropha curcas maintained on different hormonal combinations using RAPD markers. World Journal of Agricultural Sciences, 8(6): 616-623.
Kaushik, N. and Kumar, S. (2004). Jatropha curcas L. Silviculture and Uses. Agrobios (India), Jodhpur.
Kumar, A. and Sharma, S. (2008). An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.): a review. Ind. Crops Prod., 28:1-10.
Kumar, N. and Reddy, M.P. (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Ann. Appl. Biol., 156:367-375.
Kumari, N. and Jaiwal, V.S. (1998). Induction of somatic embryogenesis and plant regeneration from leaf callus of Terminalia arjuna Bedd. Current Science, 75:1052-1055.
Liu, C., Xia, X., Yin, W., Hung, L. and Zhou, J. (2006). Shoot regeneration and somatic embryogenesis from needles of redwood (Sequoia sempervirens). Plant Cell Rep., 25:621-628.
Li, M., Li, H., Jiang, H., Pan, X. and Wu, G. (2007). Establishment of an Agrobacterium-mediated cotyledon disc transformation method for Jatropha curcas. Plant Cell Tiss. Org. Cult., 92:173-181.
Menezes, R.G., Rao, N.G., Karanth, S.S., Kamath, A., Manipady, S. and Pillay, V.V. (2006). J. curcas poisoning. Ind. J. Pediatr., 73:634.
Mujib, A., Bandyopadhyay, S., Jana, B.K. and Ghosh, P.D. (1998). Direct somatic embryogenesis and in vitro plant regeneration in Hippeastrum hybridum. Plant Tissue Cult., 8(1):19-25.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plantarum, 15:473-479.
Rajore S. and Batra, A. (2007). Efficient plant regeneration via shoot tip explants in Jatropha curcas. J. Plant Biochem. Biotech., 14, 73-75.
Ramasamy, N., Ugandhar, T., Praveen, M., Venkataiah, P., Rambabu, M., Upender, M. and Subhash, K. (2005). Somatic embryogenesis and plantlet regeneration from cotyledons and leaf explants of Solanum surattense. Indian J. Biotechnology, 4:414-418.
Sahrawat, A.K. and Chand, S. (2001). Continuous somatic embryogenesis and regeneration from hypostyle segments of Psoralea corylifolia Linn: An endangered and medicinally important Fabaceae plant. Current Science, 81:1328-1331.
Shamsiah, A., Awal, A., Nurathrah, S., Khairul-Azmir, M. and Norrizah, J.S. (2010). Effects of BAP concentration on in vitro shoot multiplication and callus induction in Jatropha curcas. In: Proceedings of XXVII International Horticultural Congress on Science and Horticulture for people (IHC-2010)- International symposium on micro and macro technologies for plant propagation and breeding in horticulture, pp. 27-37.
Sardana, J., Batra, A. and Ali, D.J. (2000). An expeditious
Schekar, R.U. and Hilderbrandt, A.C. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Botany, 50:199-204.
Sridhar, T.M. and Naidu, C.V. (2011). An efficient callus induc-tion and plant regeneration of Solanum nigrum L. - An important antiulcer medicinal plant. Journal of Phytology, 3(5):23-28.
Shrivastava, S. and Banerjee, M. (2008). In vitro clonal propa-gation of physic nut (Jatropha curcas L.): Influence of additives. Int. J. Integr. Biol., 3:73-79.
Sujatha, M. and Mukta, N. (1996). Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tiss. Org. Cult., 44:135-141.
Thepsamran, N., Thepsithar, C. and Thongpukdee, A. (2008). In vitro induction of shoots and roots from J. curcas L. explants. J. of Hort. Science and Biotechnol-ogy, 83: 106-112.
Thomas, R., Sah, N.K. and Sharma, P.B. (2008). Therapeutic biology of Jatropha curcas: a mini review. Current Pharm. Biotechnol., 9:315-24.
Research Articles

How to Cite

Effects of different plant growth regulators on in vitro callus induction in physic nut (Jatropha curcus L.). (2015). Journal of Applied and Natural Science, 7(1), 30-37. https://doi.org/10.31018/jans.v7i1.559