Establishing surface sterilization protocol for clonal apple rootstock MM106
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Abstract
Surface sterilization is crucial in preparing viable and uncontaminated explants for tissue culture. Utilizing the right sterilizing agent in this procedure can efficiently eliminate the majority of surface contaminants. The present study aimed to establish the best sterilization procedure for clonal apple rootstock MM106 using shoot tips and nodal segments. Two important sterilizing agents, mercuric chloride (ranging from 0.05% to 0.3%) for various durations (2 to 6 minutes) and sodium hypochlorite (ranging from 3.0% to 12%) for different durations (10 to 30 minutes), were employed. The results indicated that the maximum aseptic conditions (80.00% and 80.67%) and survival rates of the cultures (60.66% and 67.33%) for shoot tips and nodal segments, respectively, were achieved through surface sterilization with 0.1% mercuric chloride for 4 minutes, specifically during April, in comparison to March and May of 2021 due to low phenol content and high meristematic activity. The results yielded valuable insights for efficiently propagating clonal apple rootstocks on a local scale, and they have the potential to offer guidance for establishing commercial facilities dedicated to producing these specific clonal apple rootstocks and varieties.
Article Details
Article Details
Apple, Clonal rootstock, Explants, Surface sterilization, MM106
Ghanbari, A. (2014). Impacts of plant growth regulators and culture media on in vitro propagation of three apple (Malus × domestica Borkh.) rootstocks. Iranian Journal of Genetics and Plant Breeding, 3, 11-20.
Hashim, M., Ahmad, B., Drouet, S., Hano, C., Abbasi, B.H. & Anjum, S. (2021). Comparative effects of different light sources on the production of key secondary metabolites in plants in vitro cultures. Plants, 10, 1521. https://doi.org/10.3390/plants10081521
Jaime, A., Silva, T.D., Gulyas, A., Magyar‑Tabori, K., Wang, M.R., Wang, Q.C. & Dobranszki, J. (2019). In vitro tissue culture of apple and other Malus species: recent advances and applications. Planta, 249, 975-1006. https://doi.org/10.1007/s00425-019-03100-x
Kaushal, N., Modgil, M., Thakur, M. & Sharma D R. (2005). In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds. Indian Journal of Experimental Biology, 43, 561-565.
Lal, M., Jamwal, M., Bakshi, P., Jasrotia, A., Sharma, N., Sharma, M., Singh, P., Sharma, S. & Kumar, S. (2021). Influence of antioxidants on in vitro culture establishment of clonal apple rootstocks. Biological Forum - An International Journal, 13, 381-385.
Lal, M., Jamwal, M., Sood, Y., Bakshi, P., Sharma, N., Sharma, S. & Kumar, S. (2023). Micropropagation of fruit crops: A review. Plant Science Today, 10, 108-117. https://doi.org/10.14719/pst.1891
Modgil, M., Sharma, T. & Thakur, M. (2009). Commercially feasible protocol for rooting and acclimatization of micropropagated apple rootstocks. Acta Horticulture, 839, 209-214. 10.17660/ActaHortic.2009.839.25
Murashige, T. (1974). Plant propagation through tissue culture. Annual Review of Plant Physiology 25, 135-166.
Rattanpal, H.S., Gill, M.I.S. & Sangwan, A.K. (2011). Micropropagation of strawberry through meristem culture. Acta Horticulture 890: 149-153. 10.17660/ActaHortic.2011.890.19
Soni, M., Thakur, M. & Modgil, M. (2010). In vitro multiplication of Merton I. 793- An apple rootstock suitable for replantation. Indian Journal of Biotechnology, 10, 362-368.
Wolella, E.K. (2017). Surface sterilization and in vitro propagation of Prunus domestica L. cv. Stanley using axillary buds as explants. Journal of Biotechnology Research, 8, 18-26.
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This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
