Muntaha A. Abdulkareem Kareem Ibrahim Mubarak


Acinetobacter baumannii is considered one of the important pathogenic bacteria and responsible for several nosocomial and community infections in humans, such as the lungs (pneumonia), the bloodstream of ICU patients, burns, surgical wounds and meningitis. During the period from September to the end of December 2022, a total of 210 blood specimens were collected inDiyala governorate  Baqubah Teaching Hospital, Iraq.To investigate the prevalence of bacterial isolates among ICU patients by identifying bacteria depending on suitable media under suitable environmental conditions for growth, morphological characteristics, biochemical tests, and confirmation of identification isolates by molecular detection of blaOXA- 51gene, antibiotic resistance, phenotypic and molecular detection of efflux pumps adeF gene, and activity of ciprofloxacin andresveratrol on gene expression. The percentage positive growth of A. baumannii isolates from blood specimens among ICU patients was 12 %, and the highest resistance of 25 isolates against fourteen types of antibiotics was equal to cefotaxime (100 % ), ticarcillin-clavulanate, and ceftriaxone ( 96 %), and ( 92 % )for each of Cefepime and Tobramycin, the lowest percentage was for doxycycline (64 % ).MIC value for ciprofloxacin and doxycycline between (8- 512 mg/ml) and ( 4 - 128 mg/ml) respectively. Molecular studies indicate the presence of blaOXA- 51gene  and adeF  in all isolates under study ( 100 % ). The treatment of resistance isolates with ciprofloxacin, resveratrol and their combination resulted in reduction of adeF gene. The resveratrol effect on efflux pumps gene adeF gene expression occurred for the first time in Iraq and even at the level of the Arab world.





Acinetobacter baumannii, AdeF gene, Antibiotic, Efflux pump inhibitors

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Research Articles

How to Cite

Detection of antibiotics resistance andefflux pumps production in clinical Acinetobacter baumannii isolates. (2023). Journal of Applied and Natural Science, 15(4), 1347-1353. https://doi.org/10.31018/jans.v15i4.4965