Molecular surveillance of Dengue Virus (DENV) and its co-infection with Chikungunya Virus (CHIKV) among febrile patients: A comparative study from South Delhi, India
Article Main
Abstract
Dengue and Chikungunya are two major arboviral infections transmitted worldwide by the mosquitoes, Aedes aegypti and Ae. albopictus. India suffers enormously with both Dengue and Chikungunya as they pose a great public health challenge. The present study aims to evaluate the prevalence of Dengue Virus (DENV), Chikungunya Virus (CHIKV) and DENV/CHIKV co-infection (by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR)/Enzyme Linked Immunosorbent Assay (ELISA), their clinical features, DENV serotypes and CHIKV specific Immunoglobulin G (IgG) within a 7 years gap in the Delhi population. The study sample included clinically suspected febrile patients (?7 days) sera collected during 2017-2018 (n=87) and during 2008-2010 (n=623) from Delhi. Captured ELISA was performed for CHIKV IgG screening and nested PCR was done for DENV serotyping. The percentage prevalence for DENV was significantly higher than CHIKV with 41.38% (n=87) and 16.1% (n=87), respectively; interestingly, DENV/CHIKV co-infection was detected in 10.34% (n=9/87) cases during 2017-2018. Similarly, a high DENV prevalence was observed during 2008-2010 with the prevalence rate of 38.3% (69/180), 34.65% (35/101) and 47.07% (161/342), respectively. DENV 1 and DENV 3 were dominant serotype during 2008-2010 and 2017-2018 respectively. We have noticed a high prevalence (36.67%, 22/60) of the CHIKV IgG antibody in the 2017-2018 samples. Joint pain was more preferential to CHIKV mono-infection and DENV/CHIKV co-infection compared to DENV mono-infection. The present study highlights the need for active surveillance simultaneously for both DENV and CHIKV and to evaluate the role of CHIKV/DENV co-infections in disease severity in the endemic regions.
Article Details
Article Details
Keywords
CHIKV, DENV, Co-infection, Clinical features, Serotype
References
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Gubler, D.J. (1998). Dengue and Dengue Hemorrhagic Fever. Clin Microbiol Rev 11:480–496
Hammon, W. M., Rundnick, A. & Sather, G. E. (1960). Viruses Associated with Epidemic Hemorrhagic Fevers of the Philippines and Thailand. Science 131:1102–1103. https://doi.org/10.1126/science.131.3407.1102
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Hisamuddin, M., Tazeen, A., Abdullah, M., Islamuddin, M., Parveen, N., Islam, A., Faizan, M. I., Hamza, A., Naqvi, I. H., Verma, H. N., Malik, A., Ahmed, A. & Parveen, S. (2018). Co-circulation of Chikungunya and Dengue viruses in Dengue endemic region of New Delhi, India during 2016. Epidemiol Infect., 146, 1642–1653. https://doi.org/1 0.1017/S0950268818001590
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Kumaria, R. (2010). Correlation of disease spectrum among four Dengue serotypes: a five years hospital based study from India. Braz J Infect Dis 14:141–146
Lanciotti, R.S., Calisher, C. H., Gubler, D. J., Chang, G.J. & Vorndam, A. V. (1992). Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J. Clin. Microbiol., 30, 545–551
Londhey, V., Agrawal, S., Vaidya, N., Kini, S., Shastri, J. S. & Sunil, S. (2016). Dengue and Chikungunya Virus Co-infections: The Inside Story. J Assoc Physicians India 64:36–40
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Mukherjee, S,, Dutta, S, K., Sengupta, S., Tripathi, A. (2017). Evidence of dengue and chikungunya virus co-infection and circulation of multiple dengue serotypes in a recent Indian outbreak. Eur. J. Clin. Microbiol. Infect. Dis., 36, 2273–2279. https://doi.org/10.1007/s10096-017-3061-1
Murhekar, M., Kanagasabai, K., Shete, V., Joshua, V., Ravi, M., Kirubakran, B. K., Ramachandran, R., Sabarinathan. & Gupta, N. (2019). Epidemiology of chikungunya based on laboratory surveillance data—India, 2016–2018. Trans. R. Soc. Trop. Med. Hyg., 113,259–262. https://doi.org/10.1093/trstmh/try141
Nayak, K., Jain, V., Kaur, M., Khan, N., Gottimukkala, K., Aggarwal,C., Sagar, R., Gupta, S., Rai, R. R., Dixit, K., Islamuddin, M., Khan, W. H., Verma, A., Maheshwari, D., Chawla, Y. M., Reddy, E. S., Panda, H., Sharma, P., Bhatnagar, P., Singh, P., Raghavendhar B, S., Patel, A. K., Ratageri, V. H., Chandele, A., Ray, P. & Murali-Krishna, K. (2020). Antibody response patterns in chikungunya febrile phase predicts protection versus progression to chronic arthritis. JCI Insight. https://doi.org/10.1172/jci.insight.130509
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Molecular surveillance of Dengue Virus (DENV) and its co-infection with Chikungunya Virus (CHIKV) among febrile patients: A comparative study from South Delhi, India. (2021). Journal of Applied and Natural Science, 13(2), 433-442. https://doi.org/10.31018/jans.v13i2.2616
