B. C. Greeshma M. Mahesh


Selenium nanoparticles have been widely used in the various areas especially in medical field for its anticancer and immune modulatory properties. To reduce the toxic chemicals released by chemical process, the present work was aimed at synthesis of selenium nanoparticles by microbes. In this study, microbes were isolated from soil samples from different areas of Bangalore and screened for the antioxidant activity by DPPH assay. The organism with highest antioxidant activity (IC50 value = 11.6µg/mL) was identified as Bacillus species. Under experimental conditions, this microbe along with sodium selenite synthesised selenium nanoparticle indicated by the colour change of the medium to reddish orange. The synthesised selenium nanoparticles were further characterised. From UV-Vis spectrophotometry, the maximum peak was obtained at 266.5nm. The FT-IR analysis showed peaks at different wavelengths with the maximum of 3200cm-1 showing the presence of alcoholic group. The shape and size of the selenium nanoparticles was also calibrated by SEM analysis as oval and 209nm-748nm respectively. The nanoparticles were further analysed for antimicrobial assays by well diffusion method against E. coli, S. aureus, P. aeruginosa, St. mutans and antifungal assay against the strains of C. albicans, A. niger and A. flavus. The highest zone of inhibition was observed against E.coli (1.7 cm) at a concentration of 400µg and maximum for C. albicans (2.5cm) at a concentration of 400µg. Microbial synthesized nanoparticles emerges as a promising medicine in curing different disorders because of its potent antioxidant and antimicrobial activity.  


Download data is not yet available.


Metrics Loading ...




Antimicrobial, Antioxidant, Inhibition, Selenium nanoparticles, UV-Vis spectrum

Ali, E. N., El-Sonbaty, S. M. and Salem, F. M. (2013). Evaluation of selenium nanoparticles as a potential chemopreventive agent against lung carcinoma, International Journal of Pharmaceutical, Biological and Chemical Sciences, 2 (4): 38-46
Helga Fernández?Llamosas, Laura Castro, María Luisa Blázquez, Eduardo Díaz and Manuel Carmona (2016). Biosynthesis of selenium nanoparticles by Azoarcus sp. Microb Cell Fact (2016) 15:109 DOI 10.1186/s12934?016?0510?y
Mahesh, M., Somashekhar, R., Preenon, Bagchi, Puttaiah, E. T. (2015) Optimization for the production of extracellular alkaline phosphatase from Proteus mirabilis. J Bioprocess Biotech, 5: 213 doi: 10.4172/2155-9821.1000213 P
Mallmann, E. J., Cunha, F.A., Castro, B.N., Maciel, A.M., Menezes, E.A., Fechine, P.B. (2015). Antifungal activity of silver nanoparticles obtained by green synthesis. Rev Inst Med Trop Sao Paulo. 2015;57(2):165–167.doi:10.1590/S0036-46652015000200011
Nidhi Singh, Prasenjit Saha, Karthik Rajkumar and Jayanthi Abraham (2014). Biosynthesis of silver and selenium nanoparticles by Bacillus sp. JAPSK2 and evaluation of antimicrobial activity, Der Pharmacia Lettre, 6 (1):175-181
Parisa Jafari Fesharaki, Pardis Nazari, Mojtaba Shakibaie, Sassan Rezaie, Maryam Banoee, Mohammad Abdollahi, Ahmad Reza Shahverdi (2010). Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process, Brazilian Journal of Microbiology, 41: 461-466
Ramamurthy. C. H., Sampath, K.S., Arunkumar, P., Suresh Kumar, M., Sujatha, V. (2013). Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst. Eng., 36: 1131-1139.
Shamaila Shahzadi, Nosheen Zafar and Rehana Sharif (2018). Antibacterial activity of metallic nanoparticles, Bacterial Pathogenesis and Antibacterial Control, Sahra, IntechOpen, DOI: 10.5772/intechopen.72526.
Shubharani, R., Mahesh, M. and V. N. Yogananda Murthy (2019). Biosynthesis and characterization, antioxidant and antimicrobial activities of selenium nanoparticles from ethanol extract of Bee Propolis. J Nanomed Nanotechnol.10:1 DOI: 10.4172/2157-7439.1000522
Wenjie Zhang, Zhijuan Chena, Hao Liu, Liang Zhang, Ping Gao, Daping Li (2011). Biosynthesis and structural characteristics of Selenium nanoparticles by Pseudomonas alcaliphila, Colloids And Surfaces B: Biointerfaces 88: 96– 201 doi:10.1016/j.colsurfb.2011.06.031
Xiangqian Li, Huizhong Xu, Zhe-Sheng Chen, and Guofang Chen (2011). Biosynthesis of nanoparticles by microorganisms and their applications. Journal of Nanomaterials, Volume 2011, Article ID 270974, 16 pages doi:10.1155/2011/270974
Citation Format
How to Cite
Greeshma, B. C. ., & Mahesh , M. (2019). Biosynthesis of selenium nanoparticles from Bacillus species and its applications. Journal of Applied and Natural Science, 11(4), 810–815. https://doi.org/10.31018/jans.v11i4.2188
More Citation Formats:
Research Articles