Estimation of biochemical factors responsible for resistance to root and collar rot (Macrophomina phaseolina (Tassi) Goid.) in okra
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Abstract
Twenty okra genotypes were screened for their resistance to root and collar rot incited by Macrophomina phaseolina in the experimental fields of Department of Plant Pathology, B. A. College of Agriculture, Anand Agricultural University. Based on their disease reaction, they were grouped into four categories i.e., resistant, moderately resistant, susceptible and highly susceptible. The biochemical analysis of the resistant and susceptible genotypes revealed that the total soluble sugar, reducing sugar and non reducing sugar decreased following inoculation by the pathogen. The decrease in total soluble sugar, reducing sugar and non reducing sugar were 21.54, 21.73 and 21.42 per cent in resistant variety, whereas in highly susceptible variety it was 6.06, 6.10 and 3.03 per cent, respectively. The total phenol content, soluble protein and peroxidase activity increased on inoculation with the pathogen. In the resistant variety, the increase in phenol content, soluble protein and peroxidase activity was 23.4, 32.56 and 63.86 per cent whereas in moderately resistant variety this was 19.85, 21.63 and 55.29 per cent, respectively. Susceptible and highly susceptible genotypes had low to moderate increase in the three parameters. The experimental results confirmed the importance of these biochemical factors in imparting the host resistance to root and collar rot.
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Okra, Macrophomina phaseolina, Phenol, Sugar, Protein, Peroxidase
Anonymous. 2017. Horticultural statistics at a glance (2017) Retreived from http://nhb.gov.in
Ashraf, M. S., Khan, T. A. and Hasan, S. (2005). Reaction of chickpea varieties to Macrophomina phaseolina and their effect on peroxidase activity. Pak. J. Bot., 37 (3):761-767.
Bhatnagar, R., Shukla, Y. M. and Talati, J. G. (2005). Biochemical methods for agricultural sciences. Anand Agricultural University, Department of Biochemistry. Anand.
Costa, H., Gallego, S. M. and Tomaro, M. L. (2002). Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Sci., 162: 939 – 945. PII: S 0 1 6 8 - 9 4 5 2 ( 0 2 ) 0 0 0 5 1 - 1
Doley, K. and Jite, P. K. (2013). Disease management and biochemical changes in groundnut inoculated with Glomus facsiculatum and pathogenic Macrophomina phaseolina (Tassi.) Goidanch. Plant Sci. Feed, 3 (2): 21-26. URI: http://eprints.icrisat.ac.in/id/eprint/11585
El- Fikri, A. I. I., El-Deeb, A. A., Mohammed, F. G. and Khalifa, M. M. A. (2004). Controlling sesame charcoal rot incited by Macrophomina phaseolina under field conditions by using resistant cultivars and some seed and soil treatments. Egypt. J. Phytopathol., 32 (1):103-118
Kaur, H., Sharma, S. and Saxena, A. K. (2008). Biochemical indicators for resistance to charcoal and Fusarium stalk rot in maize hybrids. J. Res. Punjab Agricultural University, 45 (3): 117-120.
Kumari, N., Sharma, I., Alam, A. and Sharma, V. (2015). Screening of sorghum genotype and biochemical changes for resistance to damage by Macrophomina phaseolina. Arch. Phytopathol. Plant Protec., 9 (12): 760-775.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein measurement with Folin phenol reagent. J. Biol. Chem., 2: 265-275.
Malik, E. P. and Singh, M. B. (1980). Plant Enzymology and Histoenzymology (1stEdn.). Kalyani Publishers, New Delhi.
Nelson, N. (1944). A photometric adoption of Somogyis’ method for determination of glucose. J. Biol. Chem., 153: 375-380.
Patil, S. H., Hedge, R. K. and Anahosur, K. H. (1985). Role of sugars and phenols in charcoal rot resistance of sorghum. J. Phytopathol., 113 (1): 30-35. DOI: 10.1111/j.1439-0434.1985.tb00821.x.
Raj, S. A and Prasad, N. N. (1975). Reaction of groundnut to Rhizoctonia bataticola (Macrophomina phaseolina). Indian Phytopathol. 28: 440-441.
Sandhu, A., Singh, R. D., Kant, U. (1998). Changes in carbohydrate contents and hydrolyzing enzymes in cowpea infected by Macrophmina phaseolina. Ann. Arid Zone, 37 (4): 423-425.
Shahabuddhin, M. and Anwar, M. M. (1992). Metabolic changes in the jute plant due to fungal infection. Bangladesh J. Bot., 21: 206-212.
Sharma, A. (2011). Effect of Macrophomina phaseolina infection in Sesamum. Paper presented at the International Conference on Issues for Climate Change, Land Use Diversification and Biotechnological Tools for Livelihood Security.
Singh SK, Nene YL, Reddy MV. (1990). Influence of cropping systems on Macrophomina phaseolina population in soil. J. Plant Dis. 74: 812-814. DOI: 10.1094/PD-74-0812
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