Present study envisaged evaluating the effect of Basic violet-1 (BV-1, CI No. 42535), a widely used azo dye in dyeing and textile industries, on antioxidant enzymes of Labeo rohita fingerlings (7.6 – 11.3 cm length and 16.1 – 26.7 g weight). Antioxidant enzymes such as acetylcholinesterase (AChE), succinate dehydrogenase (SDH), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated in liver, kidney, gill, muscle and brain of the fish as markers for the stress of BV-1. After 96h, the fish were kept for a recovery period of 30 days and activity of enzymes was determined at 15 day intervals. Significant dose dependent increase over control in the activity of AST (79.69%) and ALT (50.07%) was observed in all the tissues while a significant decrease over control was observed in AChE (717.43%) and SDH (173.07%) activity in all the tissues. Alterations in the activity of enzymes could probably be due to a defense against oxidative damage caused by the dye and prolonged effect till the end of the recovery period could be a metabolic adaptation of the fish to the stress of the present dye. The results indicate that the dye is very toxic to L. rohita as there was a marked change in the activity of selected enzymes in the exposed fish and the effect prolonged till the end of recovery period. So these enzymes in the selected tissues can be considered as best biomarker to determine toxicity of even very low doses of the azo dye BV-1 in fish.
Antioxidant enzymes, Azo dye, Basic violet-1, Detoxification, Liver, L. rohita
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