Aspergillus oryzae isolatedon Potato dextrose agar from soil samples of kottakoduru seashore of Bay of Bengal, Andhra Pradesh, India seashore of Bay of Bengal by spread plate method and was screened for alkaline protease production on Skim milk containing agar plates and identified by clear zones of protein hydrolysis around colonies. Different physical and chemical parameters such as pH, temperature, substrate concentration and incubation time were optimized for the better production of alkaline protease. The maximum protease activity was found at pH of 8 containing 10% wheat bran at 300C, after 72 hours of fermentation.ZnSO4was effective activator for protease activity and sodium dsulphate had shownmore than 50% inhibition of enzyme activity. Among the different oil cakes used for the production of enzyme the Sesame oil cake proved to be suitable substrate after wheat bran for the production of protease by Aspergillus oryzae.
Alkaline protease, Aspergillus oryzae, Temperature, Wheat bran
Anitha T.S., Palanivelu, P. (2013). Purification and characterization of an extracellular keratinolytic protease from a new isolate of Aspergillus parasiticus. Protein Expr. Purif. 88:214-220.
Banerjee, R. and Bhattacharyya, B.C.(1992). Optimization of multiple inducers effect on protease biosynthesis by Rhizopus oryzae, Bioprocess eng 7: 225-28.
Bhosale, S. H., Rao M. B., Deshpande, V.V., Srinivasan, M.C. (1995). Thermostability of high activity alkaline protease from Conidiobolus coronatus (NCl 86.8.20). Enzyme Microb. Technol., 17: 136-39.
Bayoudh, A., Gharsallah, N., Chamkha, M., Dhouib, A., Ammar, S. and Nasir, M. (2000). Purification and characterization of an alkaline protease from Pseudomonas Aeruginosa MN1. Ind. Microbiol. Biotechnol. 24: 291-95
Borriss R. (1987). Biology of enzymes. In: (Rehm H & Reed G. eds) Biotechnology. Weinheim, Verlag chemie. pp. 35-62.
Chellapandi, P. (2010). Production and preliminary characterization of alkaline protease from Aspergillus flavus and Aspergillus terreus. Journal of Chemistry, 7(2): 479-482
Chuenjit Chancharoonponga, Pao-ChuanHsiehb, Shyang-Chwen Sheub.(2012). Enzyme Production and Growth of Aspergillus oryzae on Soybean Koji Fermentation. ICBFS 2012: April 7-8, Bangkok, Thailand. APCBEE Procedia 2 57 – 61.
Chutmanop, J., Chuichlcherm, S., Chisti, Y. and Srinophokum, P.(2008). Protease production by Aspergillus oryzaein solid state fermentation using Agro industrial substrate, Chem. Technol. Biotechnol.,
Gajju, H., Bhalla T.C. and Agarwal, H.O. (1996). Thermostable alkaline protease from thermophilic Bacillus coagulans PB-77.Ind.J. Microbiol. 36:153-55
Godfrey, T. and West, S. (1996). Introduction to Industrial Enzymology. In: Godfrey T, West S (eds) Industrial enzymology, 2 nd (eds.) Macmillan Press, London, , pp 1–8
Gopalkumar G. Rao, Priyanka M. Patel, B.V. Rao and Rakeshkumar R. Pancha.(2016). Alkaline protease production by Aspergillus terreusBAB-346 using poultry litter waste. Int. J. Curr. Microbiol. App. Sci., 5(10):174-184
Gupta. R, Beg Q.K, Khan S and Chauhan B.(2002). An overview on fermentation, downstream processing and properties of microbial proteases. Appl Microbiol Biotechnol, 60: 381-395.
Ikram –Ul-Haq, Hamid Mukhtar and Hina Umber.(2006). Production of protease by Pencillium chrysogenum through optimization of environmental conditions. Journal of Agricultural and Social sciences. 1813-2235/02-1- 23-25
Kalisz, H.M. (1988). Microbial proteinases. Adv. Biochem. Eng. Biotechnol., 36: 1-65.
Karthic, J., Siddalingeshwara K.G, SunilDutt P.L.N.S.N1, Pramod, T, Vishwanatha, T.A.(2001). Approach on isolation, screening and production of protease from Aspergilus oryzae. Journal of Drug Delivery & Therapeutics; 2014, 4(2): 86-89 86 © JDDT. All Rights Reserved ISSN: 2250-1177 CODEN (USA): JDDTAO
Kaur, S., Vohra, R.M., Kapoor, M., Beg, Q.K. and Hoondal, G.S.(2001). Enhanced production and characterization of a highly thermostable alkaline protease from Bacillus sp. P-2. World Journal of Microbiology and Biotechnology, 17(2): 125-129
Kranthi, V.S., Rao, D.M. and Jaganmohan, P., (2012). Production of protease by Aspergillus flavus through solid state fermentation using different oil seed cakes. International Journal of Microbiological Research, 3(1):12-15
Kumar, C.G. and Takagi, H.(1999). Microbial alkaline proteases: from a bio-industrial viewpoint. Biotechnol. Adv. 17: 561-94.
Kumar, D.J.Mukesh, and N.Govindrajan, M.D Balakumaran and P.T.Kalaichelvan Premavathi Venkatchalam. (2002). Production and purification of Alkaline Protease from Bacillus sp. MPTK 712 isolated from Dairy sludge." IDOSI Publications 51: 433-439.
Li-Jung Yin , Ya-Hui Chou , and Shann-Tzong Jiang (2013). Purification and characterization of acidic protease from Aspergillus oryzae BCRC 30118. Journal of Marine Science and Technology, 21 (1): 105-110, DOI: 10.6119/JMST-012-0529-1
Lester, P. and Sidney, (1972). Methods in Enzymology. Academic press Inc., New York.
M. J. García-Gómez, S. Huerta-Ochoa, O. Loera-Corral, and L. A. Prado-Barragán (2009). Advantages of a proteolytic extract by Aspergillus oryzae from fish flour over a commercial proteolytic preparation, Food Chemistry, 112 (3): 604–608
Madan M, Dhillon S, Singh R.(2002)Production of alkaline protease by a UV mutant of Bacillus polymyxa. Ind. J. Microbiol., 42: 155- 159.
Malathi, S., Chakraborty, R.(1991). Production of alkaline protease by a New Aspergillus flavus isolate under solid state fermentation for use as a depilation agent, Appl. Environ. Microbiol., 57: 712- 716.
M.J.Garcia-Gomez,S.Huerta-Ochoa,O.Loera-Corral,and L.A.Prado-Barragan(2009). Advantages of a proteolytic extract by Aspergillus oryzae from fish flour over a commercial proteolytic preparation, Food Chemistry, 112 (3): 604–608
Mohanasrinivasan.V, Vani Shankar Raisha Elizabeth, Soumya A.R and C. Subathra Devi (2012). Isolation, screening and identification of protease producing fungi from rhizosphere soil and optimisation of pH, incubation Time and Inducer Concentration For Enhanced Protease Production. International Journal of Pharma and Bio Sciences, 3: 2
Muthulakshmi C, Duraisamy G, Dugganaboyana GK, Ganesan R, Manokaran K, Chandrasekar U. (2011). Production, purification and characterization of protease by Aspergillus flavus under Solid State Fermentation. Jordan J. Biol. Sci. 4(3): 137-148
Murthy, M., MadhavaNaidu,(2010).protease production by Aspergillus oryzae in solid state fermentation utilizing coffee By-products. World Applied Sciences Journal 8(2),199-205
Naidu, K.S.B. and Devi, K.L. (2005). Optimization of thermostable alkaline protease production from species of Bacillus using rice bran. African Journal of Biotechnology, 4 (7):724-726.
Negi, S. and R. Benerjee,(2006). Optimizationamylase and protease production from Aspergillusawamoriin single bioreactor through EVOP factoriadesign technique. Food Technol. Biotechnol, 44: 257-l 261.
Niyonzima, F.N. and More, S.S. (2015). Purification and characterization of detergent-compatible protease from Aspergillus terreus Gr.3, Biotech.,5(1):61-70
Osman, M.E., Khattab, O.H. and Elsaba, Y.M. (2014). Aspergillus terreus proteases: Characterization and applications. J. Chem. Bio. Phy. Sci. Sec. B, 4 (3): 2333-2346.
Pagare, R.S., Ramdasi, A.M., Khandelwal, S.R., Lokhande, M.O. and Aglave, B.A.,(2009). Production and enzyme activity of an extracellular protease from Aspergillus niger and Bacillus subtilis. International Journal of Biotechnology & Biochemistry, 5(3):3-8
SalihiPanelAhsan,AhmadAsoodeh, MansourAliabadian.(2017).Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93. International Journal of Biological Macromolecules. 94, Part B,: 827-835. https://doi.org/10.1016/j.ijbiomac.2016.06.023.
Pandey, A. (2003). Solid-state fermentation. Biochem. Eng. J., 13: 81-84
Petinate D.G., R.M. Martins, R.R.R. Coelho, M.N.L. Meirelles, M.H., Branquinha and A.B. Vermelho. (1999). Influence of growth medium in protease and pigment production by Streptomyces cyanens. Mem Inst Oswaldo Cruz , Rio de Jenerio, 94 : 173-77.
Potumarthi R., Subhakar C. and Jetty A.(2007). Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042 Effect of aeration and agitation regimes. Biochem.Engg. J., 34: 185-92 Rajkumar, Renganathan, Kothilmozhian, Ranishree, J., Ramasamy, R.. (2011). Production and Characterization of a Novel Protease from Bacillus sp. RRM1 under solid state fermentation, J. Microbiol. Biotechnol., 21: 627–636
Ramchandra, S., Singh, S., Larroche, C., Soccol, C., Pandey, A. (2007).Oil cakes and their Biotechnological applications - A Review, Biores. Technol., 98: 2000-2009.
Ruann, Janser (2014). Protease from Aspergillus oryzae: Biochemical Characterization and Application as a Potential Biocatalyst for Production of Protein Hydrolysates with Antioxidant Activities. Journal of Food Processing. Volume Article ID 372352, 11 pages.
Anwar and Saleemuddin (1998). Alkaline protease: A review. Biores. Technology, 64 (M): 175-83
Sandhya C, Sumantha A, Szakacs G and Pandey A. (2005).Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation. Process Biochem, 40, 2689– 2694.
Sharma, J., Singh, A., Kumar, R. and Mittal, A.,(2006). Partial purification of an alkaline protease from a new strain of spergillus oryzae AWT 20 and its enhanced stabilization in entrapped Ca-Alginate beads. Internet J Microbiol, 2(2): 1-14
Sethi, Sonia and Saksham Gupta (2015). Optimization of protease production from fungi isolated from soil. InJj of Applied Biol. & Pharm, 6 (3): 149-153
Sumantha, A. Sandhya, C. Szakacs, G. Soccol, C.R. Pandey, A.,(2005). Production and partial purification of a neutral metallo protease by fungal mixed substrate fermentation, Food Technol Biotechnol., 43: 313-319.
Uyar, F.; Baysal. Z. (2004). Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid-state fermentation. Process Biochem., 39: 1893-1898
Vishwantha, K.S., AppuRao, A. G. (2010).Acid protease production by solid-state fermentation using Aspergillus oryzae MTCC 5341: optimization of process parameters. J. Ind. Microbiol. Biotechnol., 37: 129-13849.Van Oort, M.(2010). Enzymes in food technology – Introduction. (eds.) Enzymes in Food Technology, second ed. Chichester: Wiley-Blackwell, 1-17
Wang, R., Chau Sing Law R and Webb C,(2005). Protease production and conidiation by Aspergillus oryzaein flour fermentation. Process Biochem, 40:217–227
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Â© Author (s)