Mycorrhizal inoculation in the plant causing increase in growth and production of phytochemicals is well reported, however little information is available related to the effect of mycorrhiza on morphological and biochemical properties of the medicinal plants like Ashwagandha. The present study is an attempt on diversity analysis in Withania somnifera with an aim to ascertain the nature and extent of genetic diversity present among different accessions in presence of mycorrhiza. The major biochemical constituents of Ashwaganda roots are with nolides which are well known for its medicinal properties.
Mycorrhizal associations confer benefits like better nutrition acquisition, enhanced growth, defense enhancement and improved abiotic and biotic stress tolerance in plants. The present investigation was undertaken to assess genetic diversity among five different accessions of W. somnifera using morphological and biochemical markers and the effect of mycorrrhizal inoculation on these marker. The present study concluded that presence of mycorrhiza was effective on plant growth and phytochemical constituents more than non-treated plants. Amongst five selected germplasms IC 283662, JA 134, RAS 23, MPAS 6 and MWS 205 of W. somnifera, JA 134 showed best response in pretext of the selected morphological and biochemical features in presence of mycorrhiza.
Arbuscular mycorrhiza, Flavonoid, Protein, Phenol, Sugar, Tannin
Allah, M., Safwat., El-Bassiouny, H., Bakry, B.A. andSadak, M.A. (2015). Effect of arbuscular mycorrhiza and glutamic acid on growth, yield, some chemical composition and nutritional quality of wheat plant grown in newly reclaimed sandy soil. RJPBCS, 6(3):1038-1054.
Al-Karaki, G.N. and Clark, R.B. (1999). Mycorrhizal influence on protein and lipid of durum wheat grown at different soil phosphorus levels. Mycorrhiza, 9:97-101
Al-Hmoud G, Al-Momany A (2017). Effect of Four Mycorrhizal Products on Squash Plant Growth and its Effect on Physiological Plant Elements. Adv Crop Sci Tech 5: 260.
Boham, B. A., Kocipai-Abyazan, R. (1974). Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium vaticulatum and V. calycinium. PacificScience 48: 458-463.
Dos Santos, E. L., Alves da Silva, F., and Barbosa da Silva, F. S. (2017). Arbuscular Mycorrhizal Fungi Increase the Phenolic Compounds Concentration in the Bark of the Stem of Libidibia Ferrea in Field Conditions. The Open Microbiology Journal, 11:283–291
Narwal, E. Annapurna K, Choudhary J and Sangwan S (2018). Effect of Arbuscular mycorrhizal Fungal Colonization on Nutrient Uptake in Rice Aerobic Conditions. Int.J.Curr.Microbiol.App.Sci. 7(04):1072-1093
Hodge, J.E. andHofreiter, B.T. (1962). Methods in Carbohydrate Chemistry, (Eds. Whistler, R.L. and Be Miller, J.N.), Academic Press, New York.
Lenin, M., Selvakumar, G., Thamizhiniyan, P. andRajendiran, R. (2010) Growth and biochemical changes of vegetable seedlings induced by arbuscular mycorrhizal fungus. J. Exp. Sci., 1(4):27-31.
Lowry, O.H., Rosbrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with Folin Phenol reagent. J. Biol. Chem., 193: 265.
Makkar, H.P.S., Blummel, M., Borowy, N.K. and Becker, K. (1993). Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J. Sci. Food Agric.,61:161-165
Manila, S. and Nelson, R. (2014). Biochemical changes induced in tomato as a result of arbuscular mycorrhizal fungal colonization and tomato wilt pathogen infection. Asian J. of Plant Sci. and Res., 4(1):62-68.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant, 15(3): 473-497.
Noori A.S., Maivan H.Z., Alaie E (2012).Changes in total phenol and flavonoid contents in Chrysanthemum leucanthemum under crude oil contamination. Adv. Environ. Biol., 6(12): 3057-3064.
Pusztahelyi, T., Holb, I. J. and Pócsi, I. (2015). Secondary metabolites in fungus-plant interactions. Front. Plant Sci,. 6:573. doi: 10.3389/fpls.2015.00573
Ratti, N. and Upadhyay, A. (2012). Effect of Glomus intraradices on growth and biochemical constituents of W. somnifera (Ashwagandha). Mycorrhiza News 24(1)
RaziaShuab (2016). Arbuscular Mycorrhizal Fungal Symbiosis with saffron (Crocus sativus L.) Plant. JNBR 5(1): 59 – 67.
Ruiz-Lozano, J.M. Mycorrhiza (2003) 13: 309. https://doi.org/10.1007/s00572-003-0237-6.
Saikia, S.K., Tiwari,S. and Pandey R. (2013). Rhizospheric biological weapons for growth enhancement andMeloidogyne incognita management in Withania somnifera cv. Poshita. Biological Control, 65 (2013) 225–234.
Tejavathi, D.H. and Jayashree, D.R. (2013). Phytochemical screening of selected medicinal herbs inoculated with arbuscular mycorrhizal fungi. IJBPAS, 2 (11): 2090-2106.
Zhang, R.Q., H. Zhub, H.Q. Zhao and Q. Yao (2013). Arbuscular mycorrhizal fungal inoculation increases phenolic synthesis in clover roots via hydrogen peroxide, salicylic acid and nitric oxide signaling pathways. J Plant Physiol. 1;170(1):74-9.
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