Evaluation of mutagenic potential of acetamiprid by dominant lethal test on Culex quinquefasciatus
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Abstract
Acetamiprid, a member of the neonicotinoid insecticide family, is a new class of insecticide which has recently entered the market place. It is very selective and provides outstanding control of sucking pests such as aphids and whiteflies of major crops. In the present investigation, dominant lethal test (DLT) is adopted for the evaluation of the genotoxic effects of acetamiprid using Culex quinquefasciatus as an experimental model. In this experiment, the males hatched from larvae treated with LD40 were cross mated with normal females and the results were based on the number of hatched and unhatched eggs laid by these females. The statistical analysis of the results for LD40 treated groups gave the values of 37.526±3.886 as against 5.23±0.77 from the nontreated groups which indicated significant dominant lethality of p<0.01. These results indicated that exposure of pesticides even at small dose level proved deleterious to the genome of mosquito and its subsequent generation.
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Keywords
Dominant lethality, Acetamiprid, Culex quinquefasciatus
References
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Anderson, D. and Conning, D. M. (1993). Experimental toxicology: The basic issue. The Royal Society of Chemistry. Cambridage. 405-440.
Bender, M. A., Bedford, J. S. and Mitchell, J. B. (1973). Mechanisms of chromosome aberration production II. Aberrations induced by 5- bromodeoxyuridine and visible light. Mutat. Res., 20: 403- 416.
Bender, M. A., Griggs, H. G. and Bedford, J. S. (1974). Mechanisms of chromosome aberration production III. Chemicals and ionizing radiation. Mutat. Res., 23: 187- 212.
Bhinder, P., Chaudhry, A. and Barna, B. (2009). rDNA ITS 2, polytene chromosomes and dominant lethal test based genotoxicity assessment oftwo pesticides using mosquito genome, Proc. International Conference on Entomology, Punjabi University, Patiala, Punjab.
Brewen, J. G., Payne, H. S., Jones, K. P. and Preston, R. J. (1975). Studies on chemically induced dominant lethality. The cytogenetic basis of MMS-induced dominant lethality in post meiotic-male germ cells. Mutat. Res., 33: 239-250.
Chaudhry, A. and Anand, P. K. (2004). Assessment of dominant lethal effects of chlorpyrifos (CPF) using mosquito genetics. Poll. Res., 23(4): 767-771.
Chaudhry, A., Bhinder, P. and Kaur, R. (2008). r-DNA-ITS 1 & 2 genes, polytene chromosomes and dominant lethal test based genotoxicity assessment of cypermethrin using mosquito genome, Proc. 2rd Chandigarh Science Congress, Panjab University, Chandigarh, p, 84.
Chaudhry, A. and Lovleen (2008). Evaluation of mutagenic potential of Carbaryl by dominant lethal test on Culex quinquefasciatus. J. Cytol. Genet., 9: 37- 44.
Elbert, A., Nauen, R. and Leicht, W. (1998). Imidacloprid, a novel chloronicotinyl insecticide: biological activity and agricultural importance. In: I. Ishaaya and D. Degheele (Eds.), Insecticides with novel modes of action: Mechanism and application (pp 50-74), Springer Verlag, Berlin, Heidelberg.
El Hassani, A. K., Dacher, M., Gary, V., Lambin, M., Gauthier, M., and Armengaud, C. (2008). Effects of sublethal doses of acetamiprid and thiamethoxam on the behavior of the honeybee (Apis mellifera). Arch. Environ. Contam. Toxicol., 54: 653–661.
Finney, D. J. (1971). Probit Analysis. Cambridge Univ Press, Cambridge.
Gaulden, M. E. and Liang, J. C. (1982). Insect cells of testing clastogenic agents. In: T. C. Hsu (Ed.), Cytogenetic assays of Environmental Mutagens (pp 107-135), Allanheld: Osmum.
Jain, A. K. and Sarbhoy, R. K. (1988). Cytogenetical studies on the effects on some chlorinated pesticides III Concluding remarks. Cytologia, 53: 427- 436.
Kocaman, A. Y. and Topaktas, M. (2007). In vitro evaluation of the genotoxicity of acetamiprid in human peripheral blood lymphocytes. Environ. Mol. Mutagen, 48: 483-490.
Kocaman, A.Y. and Topaktas, M. (2009). The in vitro genotoxic effects of a commercial formulation of á-cypermethrin in human peripheral blood lymphocytes. Environ. Mol. Mutagen, 50: 27-36.
Krishnan, K. S. (1964). A note on colonization of Culex. Bull. Wld. Hlth. Org., 31: 455- 456.
Lu, F. C. (1991). Basic toxicology fundamentals: Target organs and risk assessment (2nd ed) Hemisphere Publishing Company, New York.
Manna, G. K. and Sarkar, C. S. (1998). Mutagenic potential of the antifungal antibiotic grisefulvin to orally administered experimental mice and its follow- up in F1 and F2 generations. Perspective in Cytology and Genetics,AICCGpublic, Kalyani University, Kalyani, India. 383-393.
Mo, J., Pan, C., Zhang, S., He, H. and Cheng, J. (2005). Toxicity of acetamiprid to workers of Reticulitermes flaviceps (Isoptera: Rhinotermitidae),Copotermes formosanus (Isoptera: Rhinotermnitidae) and Odontotermes formosanus (Isoptera: Termitidae). J. Pestic. Sci., 30: 187–191.
Mo, J., Yang, T., Chenf, J. and Song, X (2008). Lethal and sublethal effects of acetamiprid on the larvae of Culex pipiens Pallens. Insect Science, 9 (3): 45- 49.
Muro, L. A. and Goyer, R. A. (1969). Chromosome damage in experimental lead poisoning. Arch Pathol., 87: 660-663.
Rust, M. K. and Saran, R. K. (2008). Toxicity, repellency, and effects of acetamiprid on western subterranean termite (Isoptera: Rhinotermitidae). J. Econ. Entomol., 101(4): 1360- 1366.
Schmuck, R. (2001). Ecotoxicological profile of the insecticide thiacloprid. Pflanzenschutz Nachr Bayer, 54:161–184.
Sharma, G. P., Chaudhry, A., Sobti, R. C., Dhar, M. and Ahluwalia, K. K.(1989). Effect of mitomycin- C on the genetics of Culex fatigans (Diptera: Culicidae). La Kromosoma.
Singh, K. R. P., Patterson, R. S., La Breque, G. C. and Razdan, R. K. (1975). Mass rearing of Cules pipiens fatigans. Weid J. Com. Dis., 731-753.
Suter, K. E. (1975). Studies on the dominant lethal and fertility effects of the heavy metal compounds methylemercuric hydroxide mercuric chloride and cadmium chloride in the male and female mice. Mut. Res., 30: 365-374.
Tomizawa, M. and Casida, J. E. (2005). Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annu. Rev. Pharmacol. Toxicol., 45:247–268.
U.S. Environmental Protection Agency (USEPA). (2002). Name of chemical: acetamiprid. Reason for issuance: conditional regis tration.(www.epa.gov/opprd001/factsheets / acetamiprid.pdf).
Yassine, A., Adessalam, K., El Hassini, Vincent, G., Catherine, A., Michel, L. and Monoque, G. (2009). Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environ. Toxicol.Chem., 28(1): 113–122.
Anderson, D. and Conning, D. M. (1993). Experimental toxicology: The basic issue. The Royal Society of Chemistry. Cambridage. 405-440.
Bender, M. A., Bedford, J. S. and Mitchell, J. B. (1973). Mechanisms of chromosome aberration production II. Aberrations induced by 5- bromodeoxyuridine and visible light. Mutat. Res., 20: 403- 416.
Bender, M. A., Griggs, H. G. and Bedford, J. S. (1974). Mechanisms of chromosome aberration production III. Chemicals and ionizing radiation. Mutat. Res., 23: 187- 212.
Bhinder, P., Chaudhry, A. and Barna, B. (2009). rDNA ITS 2, polytene chromosomes and dominant lethal test based genotoxicity assessment oftwo pesticides using mosquito genome, Proc. International Conference on Entomology, Punjabi University, Patiala, Punjab.
Brewen, J. G., Payne, H. S., Jones, K. P. and Preston, R. J. (1975). Studies on chemically induced dominant lethality. The cytogenetic basis of MMS-induced dominant lethality in post meiotic-male germ cells. Mutat. Res., 33: 239-250.
Chaudhry, A. and Anand, P. K. (2004). Assessment of dominant lethal effects of chlorpyrifos (CPF) using mosquito genetics. Poll. Res., 23(4): 767-771.
Chaudhry, A., Bhinder, P. and Kaur, R. (2008). r-DNA-ITS 1 & 2 genes, polytene chromosomes and dominant lethal test based genotoxicity assessment of cypermethrin using mosquito genome, Proc. 2rd Chandigarh Science Congress, Panjab University, Chandigarh, p, 84.
Chaudhry, A. and Lovleen (2008). Evaluation of mutagenic potential of Carbaryl by dominant lethal test on Culex quinquefasciatus. J. Cytol. Genet., 9: 37- 44.
Elbert, A., Nauen, R. and Leicht, W. (1998). Imidacloprid, a novel chloronicotinyl insecticide: biological activity and agricultural importance. In: I. Ishaaya and D. Degheele (Eds.), Insecticides with novel modes of action: Mechanism and application (pp 50-74), Springer Verlag, Berlin, Heidelberg.
El Hassani, A. K., Dacher, M., Gary, V., Lambin, M., Gauthier, M., and Armengaud, C. (2008). Effects of sublethal doses of acetamiprid and thiamethoxam on the behavior of the honeybee (Apis mellifera). Arch. Environ. Contam. Toxicol., 54: 653–661.
Finney, D. J. (1971). Probit Analysis. Cambridge Univ Press, Cambridge.
Gaulden, M. E. and Liang, J. C. (1982). Insect cells of testing clastogenic agents. In: T. C. Hsu (Ed.), Cytogenetic assays of Environmental Mutagens (pp 107-135), Allanheld: Osmum.
Jain, A. K. and Sarbhoy, R. K. (1988). Cytogenetical studies on the effects on some chlorinated pesticides III Concluding remarks. Cytologia, 53: 427- 436.
Kocaman, A. Y. and Topaktas, M. (2007). In vitro evaluation of the genotoxicity of acetamiprid in human peripheral blood lymphocytes. Environ. Mol. Mutagen, 48: 483-490.
Kocaman, A.Y. and Topaktas, M. (2009). The in vitro genotoxic effects of a commercial formulation of á-cypermethrin in human peripheral blood lymphocytes. Environ. Mol. Mutagen, 50: 27-36.
Krishnan, K. S. (1964). A note on colonization of Culex. Bull. Wld. Hlth. Org., 31: 455- 456.
Lu, F. C. (1991). Basic toxicology fundamentals: Target organs and risk assessment (2nd ed) Hemisphere Publishing Company, New York.
Manna, G. K. and Sarkar, C. S. (1998). Mutagenic potential of the antifungal antibiotic grisefulvin to orally administered experimental mice and its follow- up in F1 and F2 generations. Perspective in Cytology and Genetics,AICCGpublic, Kalyani University, Kalyani, India. 383-393.
Mo, J., Pan, C., Zhang, S., He, H. and Cheng, J. (2005). Toxicity of acetamiprid to workers of Reticulitermes flaviceps (Isoptera: Rhinotermitidae),Copotermes formosanus (Isoptera: Rhinotermnitidae) and Odontotermes formosanus (Isoptera: Termitidae). J. Pestic. Sci., 30: 187–191.
Mo, J., Yang, T., Chenf, J. and Song, X (2008). Lethal and sublethal effects of acetamiprid on the larvae of Culex pipiens Pallens. Insect Science, 9 (3): 45- 49.
Muro, L. A. and Goyer, R. A. (1969). Chromosome damage in experimental lead poisoning. Arch Pathol., 87: 660-663.
Rust, M. K. and Saran, R. K. (2008). Toxicity, repellency, and effects of acetamiprid on western subterranean termite (Isoptera: Rhinotermitidae). J. Econ. Entomol., 101(4): 1360- 1366.
Schmuck, R. (2001). Ecotoxicological profile of the insecticide thiacloprid. Pflanzenschutz Nachr Bayer, 54:161–184.
Sharma, G. P., Chaudhry, A., Sobti, R. C., Dhar, M. and Ahluwalia, K. K.(1989). Effect of mitomycin- C on the genetics of Culex fatigans (Diptera: Culicidae). La Kromosoma.
Singh, K. R. P., Patterson, R. S., La Breque, G. C. and Razdan, R. K. (1975). Mass rearing of Cules pipiens fatigans. Weid J. Com. Dis., 731-753.
Suter, K. E. (1975). Studies on the dominant lethal and fertility effects of the heavy metal compounds methylemercuric hydroxide mercuric chloride and cadmium chloride in the male and female mice. Mut. Res., 30: 365-374.
Tomizawa, M. and Casida, J. E. (2005). Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annu. Rev. Pharmacol. Toxicol., 45:247–268.
U.S. Environmental Protection Agency (USEPA). (2002). Name of chemical: acetamiprid. Reason for issuance: conditional regis tration.(www.epa.gov/opprd001/factsheets / acetamiprid.pdf).
Yassine, A., Adessalam, K., El Hassini, Vincent, G., Catherine, A., Michel, L. and Monoque, G. (2009). Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environ. Toxicol.Chem., 28(1): 113–122.
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Evaluation of mutagenic potential of acetamiprid by dominant lethal test on Culex quinquefasciatus. (2011). Journal of Applied and Natural Science, 3(2), 171-175. https://doi.org/10.31018/jans.v3i2.176
